Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1532 and DvMF_1533 are separated by 87 nucleotides DvMF_1533 and DvMF_1534 overlap by 17 nucleotides DvMF_1534 and DvMF_1535 are separated by 330 nucleotides
DvMF_1532: DvMF_1532 - carboxynorspermidine decarboxylase (RefSeq), at 1,889,088 to 1,890,305
_1532
DvMF_1533: DvMF_1533 - hypothetical protein (RefSeq), at 1,890,393 to 1,891,082
_1533
DvMF_1534: DvMF_1534 - hypothetical protein (RefSeq), at 1,891,066 to 1,891,599
_1534
DvMF_1535: DvMF_1535 - Saccharopine dehydrogenase (RefSeq), at 1,891,930 to 1,893,120
_1535
Position (kb)
1890
1891
1892 Strain fitness (log2 ratio)
-2
-1
0
1 at 1889.482 kb on + strand, within DvMF_1532 at 1889.482 kb on + strand, within DvMF_1532 at 1889.507 kb on + strand, within DvMF_1532 at 1889.533 kb on - strand, within DvMF_1532 at 1889.558 kb on - strand, within DvMF_1532 at 1889.723 kb on - strand, within DvMF_1532 at 1889.863 kb on + strand, within DvMF_1532 at 1890.074 kb on + strand, within DvMF_1532 at 1890.197 kb on - strand at 1890.309 kb on + strand at 1890.390 kb on + strand at 1890.570 kb on - strand, within DvMF_1533 at 1890.664 kb on + strand, within DvMF_1533 at 1890.720 kb on + strand, within DvMF_1533 at 1890.815 kb on + strand, within DvMF_1533 at 1891.063 kb on + strand at 1891.691 kb on + strand at 1891.771 kb on - strand at 1891.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 1,889,482 + DvMF_1532 0.32 -2.4 1,889,482 + DvMF_1532 0.32 -1.4 1,889,507 + DvMF_1532 0.34 -1.6 1,889,533 - DvMF_1532 0.37 -1.5 1,889,558 - DvMF_1532 0.39 -2.6 1,889,723 - DvMF_1532 0.52 -1.2 1,889,863 + DvMF_1532 0.64 -2.4 1,890,074 + DvMF_1532 0.81 -0.7 1,890,197 - -1.2 1,890,309 + -0.7 1,890,390 + -0.8 1,890,570 - DvMF_1533 0.26 -0.6 1,890,664 + DvMF_1533 0.39 -0.6 1,890,720 + DvMF_1533 0.47 -0.6 1,890,815 + DvMF_1533 0.61 +0.8 1,891,063 + -0.3 1,891,691 + -0.8 1,891,771 - -0.6 1,891,927 - -0.6
Or see this region's nucleotide sequence