Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28540 and RR42_RS28545 are separated by 60 nucleotides RR42_RS28545 and RR42_RS28550 are separated by 10 nucleotides RR42_RS28550 and RR42_RS28555 overlap by 4 nucleotides
RR42_RS28540: RR42_RS28540 - glutamate--pyruvate aminotransferase, at 1,757,844 to 1,759,100
_RS28540
RR42_RS28545: RR42_RS28545 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 1,759,161 to 1,760,315
_RS28545
RR42_RS28550: RR42_RS28550 - enoyl-CoA hydratase, at 1,760,326 to 1,761,096
_RS28550
RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992
_RS28555
Position (kb)
1759
1760
1761 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1758.238 kb on - strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.248 kb on + strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.249 kb on - strand, within RR42_RS28540 at 1758.252 kb on + strand, within RR42_RS28540 at 1758.253 kb on - strand, within RR42_RS28540 at 1758.348 kb on + strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.349 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.456 kb on - strand, within RR42_RS28540 at 1758.657 kb on + strand, within RR42_RS28540 at 1758.666 kb on + strand, within RR42_RS28540 at 1758.667 kb on - strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.750 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.828 kb on + strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.829 kb on - strand, within RR42_RS28540 at 1758.831 kb on - strand, within RR42_RS28540 at 1758.942 kb on + strand, within RR42_RS28540 at 1758.943 kb on - strand, within RR42_RS28540 at 1758.954 kb on + strand, within RR42_RS28540 at 1759.112 kb on + strand at 1759.112 kb on + strand at 1759.113 kb on - strand at 1759.460 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.780 kb on + strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.781 kb on - strand, within RR42_RS28545 at 1759.970 kb on - strand, within RR42_RS28545 at 1760.050 kb on + strand, within RR42_RS28545 at 1760.051 kb on - strand, within RR42_RS28545 at 1760.327 kb on + strand at 1760.328 kb on - strand at 1760.487 kb on - strand, within RR42_RS28550 at 1760.793 kb on - strand, within RR42_RS28550 at 1760.992 kb on + strand, within RR42_RS28550 at 1761.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,758,238 - RR42_RS28540 0.31 -0.1 1,758,248 + RR42_RS28540 0.32 -1.7 1,758,248 + RR42_RS28540 0.32 -1.1 1,758,248 + RR42_RS28540 0.32 -0.3 1,758,248 + RR42_RS28540 0.32 -0.0 1,758,248 + RR42_RS28540 0.32 -0.1 1,758,249 - RR42_RS28540 0.32 +0.0 1,758,249 - RR42_RS28540 0.32 +2.7 1,758,249 - RR42_RS28540 0.32 +0.5 1,758,252 + RR42_RS28540 0.32 +3.7 1,758,253 - RR42_RS28540 0.33 -1.4 1,758,348 + RR42_RS28540 0.40 +2.4 1,758,349 - RR42_RS28540 0.40 -1.3 1,758,349 - RR42_RS28540 0.40 +0.8 1,758,456 - RR42_RS28540 0.49 +0.6 1,758,456 - RR42_RS28540 0.49 -2.6 1,758,657 + RR42_RS28540 0.65 +0.2 1,758,666 + RR42_RS28540 0.65 +1.2 1,758,667 - RR42_RS28540 0.65 +1.1 1,758,750 + RR42_RS28540 0.72 -2.7 1,758,750 + RR42_RS28540 0.72 +0.4 1,758,828 + RR42_RS28540 0.78 -3.5 1,758,828 + RR42_RS28540 0.78 -3.3 1,758,828 + RR42_RS28540 0.78 -2.6 1,758,828 + RR42_RS28540 0.78 -0.4 1,758,829 - RR42_RS28540 0.78 +1.9 1,758,829 - RR42_RS28540 0.78 +1.4 1,758,831 - RR42_RS28540 0.79 -2.9 1,758,942 + RR42_RS28540 0.87 +0.9 1,758,943 - RR42_RS28540 0.87 +1.2 1,758,954 + RR42_RS28540 0.88 +0.1 1,759,112 + -0.7 1,759,112 + +1.0 1,759,113 - -0.4 1,759,460 + RR42_RS28545 0.26 -0.6 1,759,780 + RR42_RS28545 0.54 -1.3 1,759,780 + RR42_RS28545 0.54 +0.0 1,759,781 - RR42_RS28545 0.54 -4.1 1,759,781 - RR42_RS28545 0.54 -2.7 1,759,781 - RR42_RS28545 0.54 -1.6 1,759,970 - RR42_RS28545 0.70 -2.1 1,760,050 + RR42_RS28545 0.77 -3.4 1,760,051 - RR42_RS28545 0.77 -0.2 1,760,327 + +1.2 1,760,328 - +0.9 1,760,487 - RR42_RS28550 0.21 -1.9 1,760,793 - RR42_RS28550 0.61 -2.1 1,760,992 + RR42_RS28550 0.86 +0.5 1,761,121 + +0.8
Or see this region's nucleotide sequence