Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28290 and RR42_RS28295 are separated by 88 nucleotides RR42_RS28295 and RR42_RS28300 overlap by 4 nucleotides
RR42_RS28290: RR42_RS28290 - DNA-binding protein, at 1,707,565 to 1,708,161
_RS28290
RR42_RS28295: RR42_RS28295 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 1,708,250 to 1,709,452
_RS28295
RR42_RS28300: RR42_RS28300 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data) , at 1,709,449 to 1,710,423
_RS28300
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1707.353 kb on + strand at 1707.354 kb on - strand at 1707.360 kb on - strand at 1707.360 kb on - strand at 1707.374 kb on - strand at 1707.479 kb on - strand at 1707.575 kb on + strand at 1707.794 kb on + strand, within RR42_RS28290 at 1707.794 kb on + strand, within RR42_RS28290 at 1707.794 kb on + strand, within RR42_RS28290 at 1707.795 kb on - strand, within RR42_RS28290 at 1708.040 kb on - strand, within RR42_RS28290 at 1708.271 kb on + strand at 1708.272 kb on - strand at 1708.272 kb on - strand at 1708.360 kb on + strand at 1708.360 kb on + strand at 1708.409 kb on + strand, within RR42_RS28295 at 1708.409 kb on + strand, within RR42_RS28295 at 1708.410 kb on - strand, within RR42_RS28295 at 1708.498 kb on + strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.499 kb on - strand, within RR42_RS28295 at 1708.508 kb on - strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1708.693 kb on + strand, within RR42_RS28295 at 1709.014 kb on + strand, within RR42_RS28295 at 1709.140 kb on + strand, within RR42_RS28295 at 1709.177 kb on + strand, within RR42_RS28295 at 1709.252 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.306 kb on + strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.307 kb on - strand, within RR42_RS28295 at 1709.324 kb on + strand, within RR42_RS28295 at 1709.469 kb on - strand at 1709.530 kb on + strand at 1709.665 kb on + strand, within RR42_RS28300 at 1709.666 kb on - strand, within RR42_RS28300 at 1709.786 kb on + strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1709.787 kb on - strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.049 kb on + strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.050 kb on - strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.091 kb on + strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.092 kb on - strand, within RR42_RS28300 at 1710.167 kb on - strand, within RR42_RS28300 at 1710.248 kb on - strand, within RR42_RS28300
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,707,353 + +0.6 1,707,354 - -1.5 1,707,360 - +0.2 1,707,360 - +0.7 1,707,374 - -3.3 1,707,479 - -0.4 1,707,575 + -4.5 1,707,794 + RR42_RS28290 0.38 -3.5 1,707,794 + RR42_RS28290 0.38 -3.3 1,707,794 + RR42_RS28290 0.38 -3.8 1,707,795 - RR42_RS28290 0.39 -5.2 1,708,040 - RR42_RS28290 0.80 -3.4 1,708,271 + -1.7 1,708,272 - +1.0 1,708,272 - -2.4 1,708,360 + +1.4 1,708,360 + -1.6 1,708,409 + RR42_RS28295 0.13 +0.9 1,708,409 + RR42_RS28295 0.13 -2.5 1,708,410 - RR42_RS28295 0.13 -2.3 1,708,498 + RR42_RS28295 0.21 -1.0 1,708,499 - RR42_RS28295 0.21 -0.0 1,708,499 - RR42_RS28295 0.21 -1.4 1,708,508 - RR42_RS28295 0.21 -3.1 1,708,693 + RR42_RS28295 0.37 +1.4 1,708,693 + RR42_RS28295 0.37 -3.5 1,709,014 + RR42_RS28295 0.64 +0.9 1,709,140 + RR42_RS28295 0.74 -3.5 1,709,177 + RR42_RS28295 0.77 +0.7 1,709,252 + RR42_RS28295 0.83 -2.0 1,709,306 + RR42_RS28295 0.88 -1.7 1,709,306 + RR42_RS28295 0.88 +0.8 1,709,306 + RR42_RS28295 0.88 -3.5 1,709,307 - RR42_RS28295 0.88 -1.1 1,709,307 - RR42_RS28295 0.88 +0.6 1,709,307 - RR42_RS28295 0.88 -1.7 1,709,324 + RR42_RS28295 0.89 +0.2 1,709,469 - -0.1 1,709,530 + -2.2 1,709,665 + RR42_RS28300 0.22 -2.0 1,709,666 - RR42_RS28300 0.22 -2.0 1,709,786 + RR42_RS28300 0.35 -4.0 1,709,787 - RR42_RS28300 0.35 +2.8 1,709,787 - RR42_RS28300 0.35 +0.4 1,709,787 - RR42_RS28300 0.35 -0.1 1,709,787 - RR42_RS28300 0.35 -2.4 1,710,049 + RR42_RS28300 0.62 -3.6 1,710,049 + RR42_RS28300 0.62 -2.4 1,710,050 - RR42_RS28300 0.62 -1.3 1,710,050 - RR42_RS28300 0.62 +1.3 1,710,050 - RR42_RS28300 0.62 +0.0 1,710,091 + RR42_RS28300 0.66 -0.5 1,710,091 + RR42_RS28300 0.66 -0.4 1,710,091 + RR42_RS28300 0.66 -0.6 1,710,091 + RR42_RS28300 0.66 +0.4 1,710,092 - RR42_RS28300 0.66 +0.1 1,710,092 - RR42_RS28300 0.66 -4.9 1,710,167 - RR42_RS28300 0.74 -3.0 1,710,248 - RR42_RS28300 0.82 +0.3
Or see this region's nucleotide sequence