Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27835 and RR42_RS27840 are separated by 37 nucleotides RR42_RS27840 and RR42_RS27845 are separated by 65 nucleotides RR42_RS27845 and RR42_RS27850 are separated by 63 nucleotides
RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168
_RS27835
RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054
_RS27840
RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509
_RS27845
RR42_RS27850: RR42_RS27850 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 1,611,573 to 1,612,376
_RS27850
Position (kb)
1610
1611
1612 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.695 kb on + strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.696 kb on - strand, within RR42_RS27835 at 1609.923 kb on + strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.924 kb on - strand, within RR42_RS27835 at 1609.938 kb on + strand, within RR42_RS27835 at 1609.939 kb on - strand, within RR42_RS27835 at 1610.147 kb on + strand at 1610.148 kb on - strand at 1610.216 kb on + strand at 1610.216 kb on + strand at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.474 kb on - strand, within RR42_RS27840 at 1610.603 kb on - strand, within RR42_RS27840 at 1610.897 kb on - strand, within RR42_RS27840 at 1610.972 kb on - strand at 1610.973 kb on - strand at 1611.142 kb on - strand at 1611.298 kb on - strand, within RR42_RS27845 at 1611.298 kb on - strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.407 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.468 kb on + strand, within RR42_RS27845 at 1611.690 kb on + strand, within RR42_RS27850 at 1611.811 kb on - strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.955 kb on - strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,609,695 + RR42_RS27835 0.68 +0.4 1,609,695 + RR42_RS27835 0.68 -0.5 1,609,696 - RR42_RS27835 0.68 -2.4 1,609,696 - RR42_RS27835 0.68 +0.1 1,609,923 + RR42_RS27835 0.83 -4.2 1,609,924 - RR42_RS27835 0.83 +0.3 1,609,924 - RR42_RS27835 0.83 -1.3 1,609,924 - RR42_RS27835 0.83 -2.9 1,609,938 + RR42_RS27835 0.84 -1.8 1,609,939 - RR42_RS27835 0.84 +0.9 1,610,147 + +1.0 1,610,148 - +1.9 1,610,216 + -0.9 1,610,216 + -0.5 1,610,431 + RR42_RS27840 0.27 +1.8 1,610,431 + RR42_RS27840 0.27 +0.5 1,610,431 + RR42_RS27840 0.27 -0.0 1,610,431 + RR42_RS27840 0.27 +0.7 1,610,474 - RR42_RS27840 0.32 -3.6 1,610,603 - RR42_RS27840 0.47 -0.8 1,610,897 - RR42_RS27840 0.81 +0.6 1,610,972 - -3.8 1,610,973 - +3.1 1,611,142 - -1.7 1,611,298 - RR42_RS27845 0.46 +0.1 1,611,298 - RR42_RS27845 0.46 -3.8 1,611,406 + RR42_RS27845 0.73 +0.3 1,611,406 + RR42_RS27845 0.73 -3.0 1,611,406 + RR42_RS27845 0.73 -1.3 1,611,407 - RR42_RS27845 0.74 +1.1 1,611,409 - RR42_RS27845 0.74 -2.0 1,611,409 - RR42_RS27845 0.74 -1.4 1,611,468 + RR42_RS27845 0.89 -2.4 1,611,690 + RR42_RS27850 0.15 -2.7 1,611,811 - RR42_RS27850 0.30 +2.2 1,611,954 + RR42_RS27850 0.47 -0.0 1,611,954 + RR42_RS27850 0.47 +1.4 1,611,955 - RR42_RS27850 0.48 +1.7 1,611,969 + RR42_RS27850 0.49 -3.6 1,611,969 + RR42_RS27850 0.49 -0.6
Or see this region's nucleotide sequence