Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27115 and RR42_RS27120 overlap by 20 nucleotides RR42_RS27120 and RR42_RS27125 overlap by 4 nucleotides RR42_RS27125 and RR42_RS27130 are separated by 161 nucleotides
RR42_RS27115: RR42_RS27115 - amino acid ABC transporter ATP-binding protein, at 1,435,985 to 1,436,713
_RS27115
RR42_RS27120: RR42_RS27120 - amino acid ABC transporter permease, at 1,436,694 to 1,437,350
_RS27120
RR42_RS27125: RR42_RS27125 - polar amino acid ABC transporter permease, at 1,437,347 to 1,438,012
_RS27125
RR42_RS27130: RR42_RS27130 - amino acid ABC transporter substrate-binding protein, at 1,438,174 to 1,438,956
_RS27130
Position (kb)
1437
1438
1439 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1436.536 kb on + strand, within RR42_RS27115 at 1436.569 kb on + strand, within RR42_RS27115 at 1436.954 kb on - strand, within RR42_RS27120 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.420 kb on + strand, within RR42_RS27125 at 1437.421 kb on - strand, within RR42_RS27125 at 1437.834 kb on + strand, within RR42_RS27125 at 1437.835 kb on - strand, within RR42_RS27125 at 1438.005 kb on + strand at 1438.005 kb on + strand at 1438.073 kb on - strand at 1438.073 kb on - strand at 1438.172 kb on + strand at 1438.454 kb on + strand, within RR42_RS27130 at 1438.454 kb on + strand, within RR42_RS27130 at 1438.593 kb on + strand, within RR42_RS27130 at 1438.595 kb on + strand, within RR42_RS27130 at 1438.608 kb on - strand, within RR42_RS27130 at 1438.608 kb on - strand, within RR42_RS27130 at 1438.694 kb on + strand, within RR42_RS27130 at 1438.957 kb on - strand at 1438.957 kb on - strand at 1438.969 kb on + strand at 1438.969 kb on + strand at 1438.970 kb on - strand at 1438.970 kb on - strand at 1439.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,436,536 + RR42_RS27115 0.76 +0.3 1,436,569 + RR42_RS27115 0.80 -1.7 1,436,954 - RR42_RS27120 0.40 -1.1 1,437,420 + RR42_RS27125 0.11 -2.3 1,437,420 + RR42_RS27125 0.11 +0.5 1,437,420 + RR42_RS27125 0.11 -0.6 1,437,421 - RR42_RS27125 0.11 -2.5 1,437,834 + RR42_RS27125 0.73 +0.0 1,437,835 - RR42_RS27125 0.73 +1.5 1,438,005 + +0.2 1,438,005 + +0.4 1,438,073 - -1.8 1,438,073 - +0.9 1,438,172 + -0.1 1,438,454 + RR42_RS27130 0.36 +0.5 1,438,454 + RR42_RS27130 0.36 +1.0 1,438,593 + RR42_RS27130 0.54 +1.2 1,438,595 + RR42_RS27130 0.54 +0.7 1,438,608 - RR42_RS27130 0.55 -0.6 1,438,608 - RR42_RS27130 0.55 -0.4 1,438,694 + RR42_RS27130 0.66 -3.5 1,438,957 - +1.3 1,438,957 - +0.5 1,438,969 + +1.5 1,438,969 + +1.6 1,438,970 - -2.9 1,438,970 - +1.5 1,439,007 - -1.9
Or see this region's nucleotide sequence