Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS23490

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS23480 and RR42_RS23485 are separated by 152 nucleotidesRR42_RS23485 and RR42_RS23490 are separated by 28 nucleotidesRR42_RS23490 and RR42_RS23495 are separated by 25 nucleotidesRR42_RS23495 and RR42_RS23500 are separated by 59 nucleotides RR42_RS23480: RR42_RS23480 - 3-keto-5-aminohexanoate cleavage protein, at 594,357 to 595,190 _RS23480 RR42_RS23485: RR42_RS23485 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 595,343 to 596,128 _RS23485 RR42_RS23490: RR42_RS23490 - NADH oxidase, at 596,157 to 597,176 _RS23490 RR42_RS23495: RR42_RS23495 - benzene 1,2-dioxygenase, at 597,202 to 597,684 _RS23495 RR42_RS23500: RR42_RS23500 - benzene 1,2-dioxygenase, at 597,744 to 599,132 _RS23500 Position (kb) 596 597 598Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 595.173 kb on - strandat 595.195 kb on - strandat 595.214 kb on + strandat 595.214 kb on + strandat 595.215 kb on - strandat 595.217 kb on - strandat 595.389 kb on + strandat 595.389 kb on + strandat 595.389 kb on + strandat 595.389 kb on + strandat 595.389 kb on + strandat 595.389 kb on + strandat 595.390 kb on - strandat 595.390 kb on - strandat 595.497 kb on + strand, within RR42_RS23485at 595.674 kb on + strand, within RR42_RS23485at 595.675 kb on - strand, within RR42_RS23485at 595.675 kb on - strand, within RR42_RS23485at 596.128 kb on + strandat 596.129 kb on - strandat 596.269 kb on + strand, within RR42_RS23490at 596.269 kb on + strand, within RR42_RS23490at 596.270 kb on - strand, within RR42_RS23490at 596.270 kb on - strand, within RR42_RS23490at 596.270 kb on - strand, within RR42_RS23490at 596.324 kb on - strand, within RR42_RS23490at 596.449 kb on + strand, within RR42_RS23490at 596.449 kb on + strand, within RR42_RS23490at 596.450 kb on - strand, within RR42_RS23490at 596.450 kb on - strand, within RR42_RS23490at 596.664 kb on + strand, within RR42_RS23490at 596.665 kb on - strand, within RR42_RS23490at 596.752 kb on + strand, within RR42_RS23490at 596.752 kb on + strand, within RR42_RS23490at 596.752 kb on + strand, within RR42_RS23490at 596.752 kb on + strand, within RR42_RS23490at 596.752 kb on + strand, within RR42_RS23490at 596.753 kb on - strand, within RR42_RS23490at 596.984 kb on + strand, within RR42_RS23490at 596.985 kb on - strand, within RR42_RS23490at 596.986 kb on + strand, within RR42_RS23490at 596.987 kb on - strand, within RR42_RS23490at 597.169 kb on + strandat 597.169 kb on + strandat 597.169 kb on + strandat 597.169 kb on + strandat 597.212 kb on + strandat 597.212 kb on + strandat 597.212 kb on + strandat 597.212 kb on + strandat 597.213 kb on - strandat 597.213 kb on - strandat 597.213 kb on - strandat 597.236 kb on + strandat 597.257 kb on + strand, within RR42_RS23495at 597.303 kb on - strand, within RR42_RS23495at 597.326 kb on + strand, within RR42_RS23495at 597.327 kb on - strand, within RR42_RS23495at 597.327 kb on - strand, within RR42_RS23495at 597.599 kb on + strand, within RR42_RS23495at 597.818 kb on - strandat 598.045 kb on + strand, within RR42_RS23500at 598.102 kb on + strand, within RR42_RS23500at 598.102 kb on + strand, within RR42_RS23500

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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595,173 - -3.7
595,195 - +2.3
595,214 + -0.1
595,214 + -0.6
595,215 - -0.2
595,217 - +1.7
595,389 + -1.4
595,389 + +1.6
595,389 + +0.3
595,389 + -1.6
595,389 + -0.2
595,389 + +2.4
595,390 - -3.3
595,390 - +1.3
595,497 + RR42_RS23485 0.20 +1.4
595,674 + RR42_RS23485 0.42 +1.7
595,675 - RR42_RS23485 0.42 +1.2
595,675 - RR42_RS23485 0.42 -2.2
596,128 + -3.2
596,129 - +0.4
596,269 + RR42_RS23490 0.11 +0.3
596,269 + RR42_RS23490 0.11 +0.4
596,270 - RR42_RS23490 0.11 -0.0
596,270 - RR42_RS23490 0.11 +3.6
596,270 - RR42_RS23490 0.11 -1.0
596,324 - RR42_RS23490 0.16 +1.9
596,449 + RR42_RS23490 0.29 -0.1
596,449 + RR42_RS23490 0.29 -2.1
596,450 - RR42_RS23490 0.29 +1.0
596,450 - RR42_RS23490 0.29 -0.2
596,664 + RR42_RS23490 0.50 +1.3
596,665 - RR42_RS23490 0.50 -0.6
596,752 + RR42_RS23490 0.58 +1.1
596,752 + RR42_RS23490 0.58 -2.5
596,752 + RR42_RS23490 0.58 +3.0
596,752 + RR42_RS23490 0.58 -0.6
596,752 + RR42_RS23490 0.58 -0.2
596,753 - RR42_RS23490 0.58 -1.6
596,984 + RR42_RS23490 0.81 -2.4
596,985 - RR42_RS23490 0.81 -1.5
596,986 + RR42_RS23490 0.81 -1.7
596,987 - RR42_RS23490 0.81 +0.2
597,169 + -3.4
597,169 + -4.0
597,169 + -3.6
597,169 + -1.8
597,212 + +0.6
597,212 + -3.2
597,212 + -2.4
597,212 + -0.5
597,213 - -0.9
597,213 - +1.8
597,213 - -3.4
597,236 + +0.6
597,257 + RR42_RS23495 0.11 +0.2
597,303 - RR42_RS23495 0.21 +1.3
597,326 + RR42_RS23495 0.26 +1.2
597,327 - RR42_RS23495 0.26 +0.5
597,327 - RR42_RS23495 0.26 -0.6
597,599 + RR42_RS23495 0.82 +0.1
597,818 - -1.0
598,045 + RR42_RS23500 0.22 -2.4
598,102 + RR42_RS23500 0.26 +0.8
598,102 + RR42_RS23500 0.26 +0.8

Or see this region's nucleotide sequence