Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23105 and RR42_RS23110 are separated by 193 nucleotides RR42_RS23110 and RR42_RS23115 are separated by 149 nucleotides RR42_RS23115 and RR42_RS23120 are separated by 498 nucleotides RR42_RS23120 and RR42_RS23125 are separated by 32 nucleotides
RR42_RS23105: RR42_RS23105 - hypothetical protein, at 518,360 to 518,818
_RS23105
RR42_RS23110: RR42_RS23110 - isochorismatase, at 519,012 to 519,542
_RS23110
RR42_RS23115: RR42_RS23115 - serine hydrolase, at 519,692 to 521,401
_RS23115
RR42_RS23120: RR42_RS23120 - hypothetical protein, at 521,900 to 522,226
_RS23120
RR42_RS23125: RR42_RS23125 - hypothetical protein, at 522,259 to 522,546
_RS23125
Position (kb) 519
520
521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 518.704 kb on - strand, within RR42_RS23105 at 518.918 kb on + strand at 518.918 kb on + strand at 518.918 kb on + strand at 518.982 kb on - strand at 519.317 kb on - strand, within RR42_RS23110 at 519.363 kb on - strand, within RR42_RS23110 at 519.513 kb on + strand at 519.513 kb on + strand at 519.514 kb on - strand at 519.514 kb on - strand at 519.693 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.741 kb on + strand at 519.742 kb on - strand at 519.879 kb on - strand, within RR42_RS23115 at 519.879 kb on - strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 519.995 kb on + strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.001 kb on - strand, within RR42_RS23115 at 520.059 kb on + strand, within RR42_RS23115 at 520.128 kb on + strand, within RR42_RS23115 at 520.213 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.240 kb on - strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.398 kb on + strand, within RR42_RS23115 at 520.399 kb on - strand, within RR42_RS23115 at 520.536 kb on + strand, within RR42_RS23115 at 520.838 kb on + strand, within RR42_RS23115 at 520.839 kb on - strand, within RR42_RS23115 at 520.956 kb on + strand, within RR42_RS23115 at 520.962 kb on + strand, within RR42_RS23115 at 520.963 kb on - strand, within RR42_RS23115 at 521.145 kb on + strand, within RR42_RS23115 at 521.146 kb on - strand, within RR42_RS23115 at 521.292 kb on + strand at 521.292 kb on + strand at 521.437 kb on - strand at 521.505 kb on + strand at 521.505 kb on + strand at 521.793 kb on - strand at 521.916 kb on + strand at 521.917 kb on - strand at 521.917 kb on - strand at 522.040 kb on + strand, within RR42_RS23120 at 522.040 kb on + strand, within RR42_RS23120 at 522.040 kb on + strand, within RR42_RS23120 at 522.041 kb on - strand, within RR42_RS23120 at 522.130 kb on - strand, within RR42_RS23120 at 522.236 kb on + strand at 522.237 kb on - strand at 522.237 kb on - strand at 522.237 kb on - strand at 522.391 kb on - strand, within RR42_RS23125
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 518,704 - RR42_RS23105 0.75 -0.2 518,918 + +2.5 518,918 + -0.2 518,918 + -0.1 518,982 - -2.4 519,317 - RR42_RS23110 0.57 -0.6 519,363 - RR42_RS23110 0.66 -0.2 519,513 + -1.0 519,513 + +0.1 519,514 - -2.5 519,514 - +1.2 519,693 + +1.1 519,741 + -0.7 519,741 + +0.5 519,741 + -0.1 519,742 - -1.8 519,879 - RR42_RS23115 0.11 -1.5 519,879 - RR42_RS23115 0.11 -1.0 519,995 + RR42_RS23115 0.18 +0.7 519,995 + RR42_RS23115 0.18 -0.0 520,001 - RR42_RS23115 0.18 -0.2 520,001 - RR42_RS23115 0.18 -1.0 520,001 - RR42_RS23115 0.18 -2.6 520,059 + RR42_RS23115 0.21 -1.6 520,128 + RR42_RS23115 0.25 -2.7 520,213 - RR42_RS23115 0.30 -2.2 520,240 - RR42_RS23115 0.32 -1.2 520,240 - RR42_RS23115 0.32 +0.0 520,240 - RR42_RS23115 0.32 -0.1 520,398 + RR42_RS23115 0.41 +1.0 520,398 + RR42_RS23115 0.41 +0.4 520,399 - RR42_RS23115 0.41 +1.3 520,536 + RR42_RS23115 0.49 +0.9 520,838 + RR42_RS23115 0.67 +1.1 520,839 - RR42_RS23115 0.67 -0.7 520,956 + RR42_RS23115 0.74 -3.0 520,962 + RR42_RS23115 0.74 +0.7 520,963 - RR42_RS23115 0.74 -0.2 521,145 + RR42_RS23115 0.85 +0.1 521,146 - RR42_RS23115 0.85 -2.6 521,292 + -0.7 521,292 + +1.0 521,437 - -2.6 521,505 + +0.4 521,505 + -1.6 521,793 - -1.0 521,916 + +0.9 521,917 - -0.8 521,917 - +0.6 522,040 + RR42_RS23120 0.43 -2.1 522,040 + RR42_RS23120 0.43 -3.0 522,040 + RR42_RS23120 0.43 -1.2 522,041 - RR42_RS23120 0.43 -0.6 522,130 - RR42_RS23120 0.70 -0.8 522,236 + +0.7 522,237 - -0.9 522,237 - -3.1 522,237 - -1.2 522,391 - RR42_RS23125 0.46 +0.1
Or see this region's nucleotide sequence