Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22660 and RR42_RS22665 are separated by 176 nucleotides RR42_RS22665 and RR42_RS22670 are separated by 375 nucleotides
RR42_RS22660: RR42_RS22660 - hypothetical protein, at 417,870 to 418,847
_RS22660
RR42_RS22665: RR42_RS22665 - hypothetical protein, at 419,024 to 419,311
_RS22665
RR42_RS22670: RR42_RS22670 - penicillin acylase, at 419,687 to 422,188
_RS22670
Position (kb)
419
420 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.173 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.252 kb on - strand, within RR42_RS22660 at 418.308 kb on + strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.417 kb on - strand, within RR42_RS22660 at 418.425 kb on + strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.583 kb on + strand, within RR42_RS22660 at 418.907 kb on + strand at 418.908 kb on - strand at 418.911 kb on - strand at 418.921 kb on + strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 419.036 kb on + strand at 419.036 kb on + strand at 419.037 kb on - strand at 419.037 kb on - strand at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.357 kb on - strand at 419.415 kb on - strand at 419.536 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.790 kb on - strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.802 kb on - strand at 419.802 kb on - strand at 419.807 kb on + strand at 419.807 kb on + strand at 419.896 kb on + strand at 419.896 kb on + strand at 419.933 kb on + strand at 419.933 kb on + strand at 419.934 kb on - strand at 420.002 kb on + strand, within RR42_RS22670 at 420.003 kb on - strand, within RR42_RS22670
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 418,137 + RR42_RS22660 0.27 +1.3 418,137 + RR42_RS22660 0.27 +1.6 418,137 + RR42_RS22660 0.27 -3.8 418,173 + RR42_RS22660 0.31 -0.1 418,185 + RR42_RS22660 0.32 -3.5 418,185 + RR42_RS22660 0.32 -1.2 418,186 - RR42_RS22660 0.32 -0.0 418,186 - RR42_RS22660 0.32 -2.3 418,251 + RR42_RS22660 0.39 +3.5 418,251 + RR42_RS22660 0.39 +0.7 418,252 - RR42_RS22660 0.39 +0.2 418,308 + RR42_RS22660 0.45 +1.2 418,309 - RR42_RS22660 0.45 -0.4 418,309 - RR42_RS22660 0.45 +0.5 418,339 - RR42_RS22660 0.48 +1.3 418,339 - RR42_RS22660 0.48 -3.7 418,416 + RR42_RS22660 0.56 -0.3 418,416 + RR42_RS22660 0.56 +1.6 418,416 + RR42_RS22660 0.56 +0.6 418,416 + RR42_RS22660 0.56 +1.7 418,417 - RR42_RS22660 0.56 -0.4 418,425 + RR42_RS22660 0.57 +1.7 418,426 - RR42_RS22660 0.57 +0.4 418,426 - RR42_RS22660 0.57 -0.7 418,510 - RR42_RS22660 0.65 +0.9 418,510 - RR42_RS22660 0.65 +0.8 418,510 - RR42_RS22660 0.65 +0.5 418,510 - RR42_RS22660 0.65 +1.3 418,583 + RR42_RS22660 0.73 -2.6 418,907 + -0.7 418,908 - -2.8 418,911 - +0.6 418,921 + -0.2 418,922 - -3.4 418,922 - -3.6 418,922 - +0.4 418,947 - -0.6 418,947 - +0.7 418,947 - -0.5 419,036 + -1.5 419,036 + -5.2 419,037 - -0.8 419,037 - -1.0 419,162 + RR42_RS22665 0.48 -4.2 419,162 + RR42_RS22665 0.48 -2.7 419,162 + RR42_RS22665 0.48 -0.1 419,163 - RR42_RS22665 0.48 -0.0 419,163 - RR42_RS22665 0.48 +0.3 419,163 - RR42_RS22665 0.48 +0.8 419,163 - RR42_RS22665 0.48 +0.4 419,163 - RR42_RS22665 0.48 +0.2 419,357 - -3.6 419,415 - +0.7 419,536 - -0.4 419,775 - -1.6 419,775 - -3.6 419,775 - -0.4 419,775 - +1.9 419,775 - +1.7 419,790 - -0.9 419,801 + -2.2 419,801 + -1.3 419,801 + -3.0 419,801 + +1.1 419,801 + -0.4 419,801 + -3.5 419,802 - +0.0 419,802 - +1.1 419,807 + -0.9 419,807 + -0.7 419,896 + +0.6 419,896 + -2.9 419,933 + +1.7 419,933 + +0.6 419,934 - -2.0 420,002 + RR42_RS22670 0.13 -0.4 420,003 - RR42_RS22670 0.13 +0.9
Or see this region's nucleotide sequence