Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22485 and RR42_RS22490 are separated by 304 nucleotides RR42_RS22490 and RR42_RS22495 overlap by 4 nucleotides RR42_RS22495 and RR42_RS22500 are separated by 46 nucleotides
RR42_RS22485: RR42_RS22485 - MBL fold metallo-hydrolase, at 381,634 to 382,593
_RS22485
RR42_RS22490: RR42_RS22490 - IclR family transcriptional regulator, at 382,898 to 383,725
_RS22490
RR42_RS22495: RR42_RS22495 - hypothetical protein, at 383,722 to 384,561
_RS22495
RR42_RS22500: RR42_RS22500 - ABC transporter substrate-binding protein, at 384,608 to 385,597
_RS22500
Position (kb) 382
383
384 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 382.028 kb on + strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.029 kb on - strand, within RR42_RS22485 at 382.277 kb on + strand, within RR42_RS22485 at 382.278 kb on - strand, within RR42_RS22485 at 382.322 kb on + strand, within RR42_RS22485 at 382.323 kb on - strand, within RR42_RS22485 at 382.356 kb on - strand, within RR42_RS22485 at 382.373 kb on + strand, within RR42_RS22485 at 382.374 kb on - strand, within RR42_RS22485 at 382.512 kb on - strand at 382.517 kb on + strand at 382.517 kb on + strand at 382.518 kb on - strand at 382.695 kb on + strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.696 kb on - strand at 382.697 kb on + strand at 382.697 kb on + strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.698 kb on - strand at 382.728 kb on + strand at 382.798 kb on - strand at 382.869 kb on + strand at 382.869 kb on + strand at 382.869 kb on + strand at 382.870 kb on - strand at 382.870 kb on - strand at 382.870 kb on - strand at 382.872 kb on + strand at 382.872 kb on + strand at 382.872 kb on + strand at 382.873 kb on - strand at 382.873 kb on - strand at 382.873 kb on - strand at 382.873 kb on - strand at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.093 kb on + strand, within RR42_RS22490 at 383.094 kb on - strand, within RR42_RS22490 at 383.189 kb on + strand, within RR42_RS22490 at 383.189 kb on + strand, within RR42_RS22490 at 383.190 kb on - strand, within RR42_RS22490 at 383.190 kb on - strand, within RR42_RS22490 at 383.310 kb on - strand, within RR42_RS22490 at 383.710 kb on + strand at 384.180 kb on + strand, within RR42_RS22495 at 384.180 kb on + strand, within RR42_RS22495 at 384.328 kb on + strand, within RR42_RS22495 at 384.489 kb on + strand at 384.609 kb on + strand at 384.610 kb on - strand at 384.681 kb on + strand at 384.681 kb on + strand at 384.682 kb on - strand at 384.693 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 382,028 + RR42_RS22485 0.41 -0.9 382,029 - RR42_RS22485 0.41 +1.7 382,029 - RR42_RS22485 0.41 +1.9 382,277 + RR42_RS22485 0.67 -1.5 382,278 - RR42_RS22485 0.67 +0.4 382,322 + RR42_RS22485 0.72 +2.0 382,323 - RR42_RS22485 0.72 +0.1 382,356 - RR42_RS22485 0.75 +0.2 382,373 + RR42_RS22485 0.77 +2.3 382,374 - RR42_RS22485 0.77 -1.9 382,512 - -0.7 382,517 + +0.9 382,517 + -1.5 382,518 - +0.5 382,695 + -1.5 382,696 - +0.3 382,696 - -0.2 382,696 - +0.5 382,696 - +0.8 382,696 - -0.2 382,697 + +1.1 382,697 + -0.1 382,698 - -0.3 382,698 - +0.9 382,698 - -3.0 382,698 - -0.2 382,698 - +1.4 382,698 - +0.6 382,728 + -2.3 382,798 - -1.1 382,869 + -0.7 382,869 + -2.1 382,869 + +0.9 382,870 - +1.5 382,870 - +0.5 382,870 - -3.9 382,872 + -0.1 382,872 + +1.4 382,872 + +0.1 382,873 - -2.2 382,873 - +2.6 382,873 - -0.6 382,873 - -1.6 383,093 + RR42_RS22490 0.24 +1.2 383,093 + RR42_RS22490 0.24 -1.4 383,093 + RR42_RS22490 0.24 -2.3 383,093 + RR42_RS22490 0.24 +0.3 383,093 + RR42_RS22490 0.24 -0.6 383,094 - RR42_RS22490 0.24 -0.7 383,189 + RR42_RS22490 0.35 -0.5 383,189 + RR42_RS22490 0.35 +1.6 383,190 - RR42_RS22490 0.35 +0.5 383,190 - RR42_RS22490 0.35 +0.2 383,310 - RR42_RS22490 0.50 -3.8 383,710 + +1.4 384,180 + RR42_RS22495 0.55 -3.3 384,180 + RR42_RS22495 0.55 -3.1 384,328 + RR42_RS22495 0.72 +0.1 384,489 + -0.7 384,609 + +0.1 384,610 - +1.4 384,681 + -2.9 384,681 + +2.1 384,682 - -0.4 384,693 + +0.5
Or see this region's nucleotide sequence