Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21555 and RR42_RS21560 are separated by 11 nucleotides RR42_RS21560 and RR42_RS21565 are separated by 120 nucleotides
RR42_RS21555: RR42_RS21555 - membrane protein, at 161,530 to 162,519
_RS21555
RR42_RS21560: RR42_RS21560 - hypothetical protein, at 162,531 to 163,229
_RS21560
RR42_RS21565: RR42_RS21565 - hypothetical protein, at 163,350 to 164,309
_RS21565
Position (kb) 162
163
164 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 161.734 kb on + strand, within RR42_RS21555 at 161.735 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.831 kb on - strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 161.944 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.043 kb on + strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.044 kb on - strand, within RR42_RS21555 at 162.278 kb on + strand, within RR42_RS21555 at 162.316 kb on + strand, within RR42_RS21555 at 162.338 kb on + strand, within RR42_RS21555 at 162.387 kb on + strand, within RR42_RS21555 at 162.548 kb on - strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.580 kb on + strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.581 kb on - strand at 162.616 kb on + strand, within RR42_RS21560 at 162.617 kb on - strand, within RR42_RS21560 at 162.678 kb on + strand, within RR42_RS21560 at 162.761 kb on + strand, within RR42_RS21560 at 162.871 kb on + strand, within RR42_RS21560 at 162.872 kb on - strand, within RR42_RS21560 at 163.240 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.279 kb on - strand at 163.393 kb on + strand at 163.729 kb on + strand, within RR42_RS21565 at 163.729 kb on + strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.730 kb on - strand, within RR42_RS21565 at 163.837 kb on + strand, within RR42_RS21565 at 163.838 kb on - strand, within RR42_RS21565 at 163.838 kb on - strand, within RR42_RS21565 at 164.034 kb on + strand, within RR42_RS21565 at 164.221 kb on + strand at 164.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 161,734 + RR42_RS21555 0.21 +0.7 161,735 - RR42_RS21555 0.21 -0.3 161,831 - RR42_RS21555 0.30 -0.4 161,831 - RR42_RS21555 0.30 -0.8 161,944 + RR42_RS21555 0.42 -3.3 161,944 + RR42_RS21555 0.42 -2.7 162,043 + RR42_RS21555 0.52 +0.1 162,043 + RR42_RS21555 0.52 -1.7 162,043 + RR42_RS21555 0.52 +1.0 162,044 - RR42_RS21555 0.52 -0.1 162,044 - RR42_RS21555 0.52 -1.3 162,044 - RR42_RS21555 0.52 -0.5 162,044 - RR42_RS21555 0.52 +1.9 162,044 - RR42_RS21555 0.52 +0.5 162,278 + RR42_RS21555 0.76 -0.8 162,316 + RR42_RS21555 0.79 +1.3 162,338 + RR42_RS21555 0.82 +2.2 162,387 + RR42_RS21555 0.87 -0.3 162,548 - -0.4 162,580 + -0.7 162,580 + -3.8 162,580 + -0.4 162,580 + -1.3 162,580 + +1.7 162,581 - -0.5 162,581 - -1.7 162,581 - -3.1 162,581 - -0.4 162,581 - +2.1 162,616 + RR42_RS21560 0.12 -0.3 162,617 - RR42_RS21560 0.12 -0.6 162,678 + RR42_RS21560 0.21 +1.4 162,761 + RR42_RS21560 0.33 +1.2 162,871 + RR42_RS21560 0.49 -0.8 162,872 - RR42_RS21560 0.49 +0.8 163,240 + +1.0 163,278 + +0.2 163,278 + -3.1 163,278 + -0.4 163,279 - +1.2 163,279 - +1.3 163,279 - +0.2 163,279 - -3.7 163,393 + +0.3 163,729 + RR42_RS21565 0.39 +1.3 163,729 + RR42_RS21565 0.39 -0.5 163,730 - RR42_RS21565 0.40 +0.2 163,730 - RR42_RS21565 0.40 -0.5 163,730 - RR42_RS21565 0.40 -1.5 163,730 - RR42_RS21565 0.40 +0.1 163,837 + RR42_RS21565 0.51 +1.3 163,838 - RR42_RS21565 0.51 -0.8 163,838 - RR42_RS21565 0.51 +1.4 164,034 + RR42_RS21565 0.71 -0.3 164,221 + +0.5 164,222 - +1.8
Or see this region's nucleotide sequence