Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21130 and RR42_RS21135 are separated by 43 nucleotides RR42_RS21135 and RR42_RS21140 are separated by 307 nucleotides
RR42_RS21130: RR42_RS21130 - ABC transporter permease, at 65,756 to 66,892
_RS21130
RR42_RS21135: RR42_RS21135 - gluconate 5-dehydrogenase, at 66,936 to 67,703
_RS21135
RR42_RS21140: RR42_RS21140 - LysR family transcriptional regulator, at 68,011 to 68,904
_RS21140
Position (kb) 66
67
68 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 65.962 kb on - strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 66.076 kb on - strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.199 kb on - strand, within RR42_RS21130 at 66.209 kb on + strand, within RR42_RS21130 at 66.279 kb on - strand, within RR42_RS21130 at 66.545 kb on - strand, within RR42_RS21130 at 66.635 kb on + strand, within RR42_RS21130 at 66.788 kb on + strand at 66.890 kb on + strand at 66.925 kb on - strand at 66.926 kb on + strand at 66.941 kb on + strand at 66.941 kb on + strand at 66.942 kb on - strand at 66.942 kb on - strand at 66.942 kb on - strand at 67.241 kb on + strand, within RR42_RS21135 at 67.242 kb on - strand, within RR42_RS21135 at 67.242 kb on - strand, within RR42_RS21135 at 67.411 kb on - strand, within RR42_RS21135 at 67.418 kb on + strand, within RR42_RS21135 at 67.506 kb on + strand, within RR42_RS21135 at 67.507 kb on - strand, within RR42_RS21135 at 67.650 kb on + strand at 67.650 kb on + strand at 67.655 kb on + strand at 67.656 kb on - strand at 67.656 kb on - strand at 67.748 kb on - strand at 67.819 kb on + strand at 67.868 kb on - strand at 67.878 kb on - strand at 68.325 kb on + strand, within RR42_RS21140 at 68.326 kb on - strand, within RR42_RS21140 at 68.417 kb on - strand, within RR42_RS21140
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 65,962 - RR42_RS21130 0.18 -2.1 65,990 + RR42_RS21130 0.21 -1.3 65,990 + RR42_RS21130 0.21 -3.0 65,991 - RR42_RS21130 0.21 -1.1 65,991 - RR42_RS21130 0.21 -1.8 66,076 - RR42_RS21130 0.28 +2.2 66,198 + RR42_RS21130 0.39 -2.6 66,198 + RR42_RS21130 0.39 -0.1 66,198 + RR42_RS21130 0.39 -4.3 66,199 - RR42_RS21130 0.39 -0.1 66,209 + RR42_RS21130 0.40 -2.0 66,279 - RR42_RS21130 0.46 -2.3 66,545 - RR42_RS21130 0.69 -1.3 66,635 + RR42_RS21130 0.77 -1.7 66,788 + +0.2 66,890 + +0.9 66,925 - +0.5 66,926 + +0.5 66,941 + -2.2 66,941 + +0.3 66,942 - +1.2 66,942 - +0.1 66,942 - +0.7 67,241 + RR42_RS21135 0.40 +1.1 67,242 - RR42_RS21135 0.40 +0.9 67,242 - RR42_RS21135 0.40 +2.0 67,411 - RR42_RS21135 0.62 -2.3 67,418 + RR42_RS21135 0.63 +0.6 67,506 + RR42_RS21135 0.74 +1.1 67,507 - RR42_RS21135 0.74 +0.1 67,650 + -2.8 67,650 + +0.5 67,655 + +1.0 67,656 - +0.6 67,656 - +0.9 67,748 - -0.3 67,819 + -1.7 67,868 - +0.7 67,878 - +3.2 68,325 + RR42_RS21140 0.35 -0.4 68,326 - RR42_RS21140 0.35 -0.7 68,417 - RR42_RS21140 0.45 -0.8
Or see this region's nucleotide sequence