Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16365 and RR42_RS16370 are separated by 29 nucleotides RR42_RS16370 and RR42_RS16375 are separated by 19 nucleotides RR42_RS16375 and RR42_RS16380 are separated by 40 nucleotides
RR42_RS16365: RR42_RS16365 - 3-oxoacyl-ACP reductase, at 3,544,573 to 3,545,358
_RS16365
RR42_RS16370: RR42_RS16370 - glutamine ABC transporter ATP-binding protein, at 3,545,388 to 3,546,170
_RS16370
RR42_RS16375: RR42_RS16375 - short-chain dehydrogenase, at 3,546,190 to 3,546,963
_RS16375
RR42_RS16380: RR42_RS16380 - hypothetical protein, at 3,547,004 to 3,547,963
_RS16380
Position (kb)
3546
3547 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.242 kb on + strand, within RR42_RS16365 at 3545.243 kb on - strand, within RR42_RS16365 at 3545.292 kb on + strand at 3545.400 kb on + strand at 3545.877 kb on - strand, within RR42_RS16370 at 3545.977 kb on - strand, within RR42_RS16370 at 3546.021 kb on + strand, within RR42_RS16370 at 3546.021 kb on + strand, within RR42_RS16370 at 3546.112 kb on - strand at 3546.112 kb on - strand at 3546.329 kb on + strand, within RR42_RS16375 at 3546.476 kb on + strand, within RR42_RS16375 at 3546.553 kb on + strand, within RR42_RS16375 at 3546.554 kb on - strand, within RR42_RS16375 at 3546.624 kb on + strand, within RR42_RS16375 at 3546.714 kb on - strand, within RR42_RS16375 at 3547.017 kb on + strand at 3547.018 kb on - strand at 3547.056 kb on + strand at 3547.056 kb on + strand at 3547.056 kb on + strand at 3547.057 kb on - strand at 3547.057 kb on - strand at 3547.116 kb on + strand, within RR42_RS16380 at 3547.202 kb on + strand, within RR42_RS16380 at 3547.202 kb on + strand, within RR42_RS16380 at 3547.202 kb on + strand, within RR42_RS16380 at 3547.233 kb on + strand, within RR42_RS16380 at 3547.234 kb on - strand, within RR42_RS16380 at 3547.300 kb on - strand, within RR42_RS16380 at 3547.604 kb on - strand, within RR42_RS16380 at 3547.649 kb on + strand, within RR42_RS16380 at 3547.650 kb on - strand, within RR42_RS16380 at 3547.650 kb on - strand, within RR42_RS16380 at 3547.688 kb on - strand, within RR42_RS16380 at 3547.839 kb on - strand, within RR42_RS16380 at 3547.839 kb on - strand, within RR42_RS16380 at 3547.901 kb on + strand at 3547.949 kb on + strand at 3547.949 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,545,242 + RR42_RS16365 0.85 -3.7 3,545,242 + RR42_RS16365 0.85 +2.0 3,545,242 + RR42_RS16365 0.85 -2.1 3,545,242 + RR42_RS16365 0.85 +3.4 3,545,243 - RR42_RS16365 0.85 -0.1 3,545,292 + -3.7 3,545,400 + +0.3 3,545,877 - RR42_RS16370 0.62 -0.3 3,545,977 - RR42_RS16370 0.75 +2.2 3,546,021 + RR42_RS16370 0.81 +0.4 3,546,021 + RR42_RS16370 0.81 +0.5 3,546,112 - -2.6 3,546,112 - -3.0 3,546,329 + RR42_RS16375 0.18 -1.3 3,546,476 + RR42_RS16375 0.37 -3.5 3,546,553 + RR42_RS16375 0.47 +0.9 3,546,554 - RR42_RS16375 0.47 +0.9 3,546,624 + RR42_RS16375 0.56 +0.9 3,546,714 - RR42_RS16375 0.68 -0.1 3,547,017 + +2.0 3,547,018 - +0.7 3,547,056 + -3.3 3,547,056 + +1.1 3,547,056 + +0.2 3,547,057 - -4.4 3,547,057 - -2.9 3,547,116 + RR42_RS16380 0.12 +0.2 3,547,202 + RR42_RS16380 0.21 +0.9 3,547,202 + RR42_RS16380 0.21 -2.9 3,547,202 + RR42_RS16380 0.21 +0.5 3,547,233 + RR42_RS16380 0.24 +0.3 3,547,234 - RR42_RS16380 0.24 -0.8 3,547,300 - RR42_RS16380 0.31 -2.6 3,547,604 - RR42_RS16380 0.62 -1.2 3,547,649 + RR42_RS16380 0.67 +0.4 3,547,650 - RR42_RS16380 0.67 +0.6 3,547,650 - RR42_RS16380 0.67 -0.7 3,547,688 - RR42_RS16380 0.71 -3.7 3,547,839 - RR42_RS16380 0.87 -3.1 3,547,839 - RR42_RS16380 0.87 -1.5 3,547,901 + -0.7 3,547,949 + -0.5 3,547,949 + -2.6
Or see this region's nucleotide sequence