Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15605 and RR42_RS15610 are separated by 45 nucleotides RR42_RS15610 and RR42_RS15615 are separated by 333 nucleotides RR42_RS15615 and RR42_RS15620 are separated by 236 nucleotides RR42_RS15620 and RR42_RS15625 are separated by 75 nucleotides
RR42_RS15605: RR42_RS15605 - poly(3-hydroxybutyrate) depolymerase, at 3,371,827 to 3,373,041
_RS15605
RR42_RS15610: RR42_RS15610 - cupin, at 3,373,087 to 3,373,494
_RS15610
RR42_RS15615: RR42_RS15615 - aldose epimerase, at 3,373,828 to 3,374,763
_RS15615
RR42_RS15620: RR42_RS15620 - tRNA-Gly, at 3,375,000 to 3,375,074
_RS15620
RR42_RS15625: RR42_RS15625 - tRNA (guanine-N7)-methyltransferase, at 3,375,150 to 3,375,917
_RS15625
Position (kb)
3373
3374
3375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3372.867 kb on + strand, within RR42_RS15605 at 3372.925 kb on + strand at 3372.925 kb on + strand at 3372.926 kb on - strand at 3372.927 kb on + strand at 3372.927 kb on + strand at 3372.928 kb on - strand at 3372.928 kb on - strand at 3372.928 kb on - strand at 3372.965 kb on - strand at 3373.029 kb on + strand at 3373.030 kb on - strand at 3373.190 kb on + strand, within RR42_RS15610 at 3373.286 kb on + strand, within RR42_RS15610 at 3373.287 kb on - strand, within RR42_RS15610 at 3373.287 kb on - strand, within RR42_RS15610 at 3373.287 kb on - strand, within RR42_RS15610 at 3373.439 kb on + strand, within RR42_RS15610 at 3373.792 kb on + strand at 3373.845 kb on + strand at 3373.963 kb on + strand, within RR42_RS15615 at 3373.963 kb on + strand, within RR42_RS15615 at 3373.963 kb on + strand, within RR42_RS15615 at 3373.964 kb on - strand, within RR42_RS15615 at 3373.964 kb on - strand, within RR42_RS15615 at 3374.129 kb on + strand, within RR42_RS15615 at 3374.129 kb on + strand, within RR42_RS15615 at 3374.130 kb on - strand, within RR42_RS15615 at 3374.130 kb on - strand, within RR42_RS15615 at 3374.308 kb on + strand, within RR42_RS15615 at 3374.309 kb on - strand, within RR42_RS15615 at 3374.309 kb on - strand, within RR42_RS15615 at 3374.311 kb on + strand, within RR42_RS15615 at 3374.312 kb on - strand, within RR42_RS15615 at 3374.312 kb on - strand, within RR42_RS15615 at 3374.329 kb on + strand, within RR42_RS15615 at 3374.329 kb on + strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.330 kb on - strand, within RR42_RS15615 at 3374.447 kb on - strand, within RR42_RS15615 at 3374.648 kb on - strand, within RR42_RS15615 at 3374.762 kb on - strand at 3374.762 kb on - strand at 3374.771 kb on + strand at 3374.811 kb on - strand at 3374.924 kb on + strand at 3374.924 kb on + strand at 3374.925 kb on - strand at 3375.010 kb on + strand, within RR42_RS15620 at 3375.010 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.012 kb on + strand, within RR42_RS15620 at 3375.022 kb on + strand, within RR42_RS15620 at 3375.040 kb on + strand, within RR42_RS15620 at 3375.041 kb on - strand, within RR42_RS15620 at 3375.339 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.744 kb on + strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625 at 3375.745 kb on - strand, within RR42_RS15625
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,372,867 + RR42_RS15605 0.86 -1.0 3,372,925 + -2.0 3,372,925 + +1.7 3,372,926 - +1.0 3,372,927 + -2.0 3,372,927 + -0.1 3,372,928 - -2.1 3,372,928 - -1.7 3,372,928 - -0.4 3,372,965 - +0.0 3,373,029 + -1.4 3,373,030 - -1.7 3,373,190 + RR42_RS15610 0.25 +1.1 3,373,286 + RR42_RS15610 0.49 +1.3 3,373,287 - RR42_RS15610 0.49 -0.3 3,373,287 - RR42_RS15610 0.49 +0.6 3,373,287 - RR42_RS15610 0.49 +0.8 3,373,439 + RR42_RS15610 0.86 +1.1 3,373,792 + -1.8 3,373,845 + -1.8 3,373,963 + RR42_RS15615 0.14 -1.4 3,373,963 + RR42_RS15615 0.14 +2.0 3,373,963 + RR42_RS15615 0.14 +1.4 3,373,964 - RR42_RS15615 0.15 -2.4 3,373,964 - RR42_RS15615 0.15 -1.4 3,374,129 + RR42_RS15615 0.32 +1.2 3,374,129 + RR42_RS15615 0.32 +0.6 3,374,130 - RR42_RS15615 0.32 -1.0 3,374,130 - RR42_RS15615 0.32 -2.9 3,374,308 + RR42_RS15615 0.51 +1.4 3,374,309 - RR42_RS15615 0.51 +0.7 3,374,309 - RR42_RS15615 0.51 +0.4 3,374,311 + RR42_RS15615 0.52 +0.4 3,374,312 - RR42_RS15615 0.52 -1.8 3,374,312 - RR42_RS15615 0.52 -0.1 3,374,329 + RR42_RS15615 0.54 -0.6 3,374,329 + RR42_RS15615 0.54 +0.0 3,374,330 - RR42_RS15615 0.54 -0.6 3,374,330 - RR42_RS15615 0.54 +0.2 3,374,330 - RR42_RS15615 0.54 +0.7 3,374,447 - RR42_RS15615 0.66 -0.2 3,374,648 - RR42_RS15615 0.88 -1.8 3,374,762 - -1.3 3,374,762 - -0.1 3,374,771 + -0.8 3,374,811 - -0.0 3,374,924 + +1.0 3,374,924 + +1.0 3,374,925 - -1.3 3,375,010 + RR42_RS15620 0.13 +0.0 3,375,010 + RR42_RS15620 0.13 +0.7 3,375,012 + RR42_RS15620 0.16 -0.9 3,375,012 + RR42_RS15620 0.16 +0.9 3,375,012 + RR42_RS15620 0.16 +1.0 3,375,022 + RR42_RS15620 0.29 +0.1 3,375,040 + RR42_RS15620 0.53 +0.8 3,375,041 - RR42_RS15620 0.55 -1.4 3,375,339 + RR42_RS15625 0.25 -3.3 3,375,744 + RR42_RS15625 0.77 +0.4 3,375,744 + RR42_RS15625 0.77 -3.5 3,375,744 + RR42_RS15625 0.77 -1.6 3,375,744 + RR42_RS15625 0.77 +1.1 3,375,745 - RR42_RS15625 0.77 +2.0 3,375,745 - RR42_RS15625 0.77 -0.1 3,375,745 - RR42_RS15625 0.77 -1.0
Or see this region's nucleotide sequence