Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14020 and RR42_RS14025 overlap by 4 nucleotides RR42_RS14025 and RR42_RS14030 are separated by 30 nucleotides RR42_RS14030 and RR42_RS14035 overlap by 8 nucleotides RR42_RS14035 and RR42_RS14040 overlap by 4 nucleotides
RR42_RS14020: RR42_RS14020 - 4'-phosphopantetheinyl transferase, at 3,019,207 to 3,019,650
_RS14020
RR42_RS14025: RR42_RS14025 - pyridoxine 5'-phosphate synthase, at 3,019,647 to 3,020,429
_RS14025
RR42_RS14030: RR42_RS14030 - DNA recombination protein RecO, at 3,020,460 to 3,021,338
_RS14030
RR42_RS14035: RR42_RS14035 - GTPase Era, at 3,021,331 to 3,022,266
_RS14035
RR42_RS14040: RR42_RS14040 - ribonuclease III, at 3,022,263 to 3,023,033
_RS14040
Position (kb)
3020
3021
3022 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3019.643 kb on + strand at 3019.644 kb on - strand at 3019.672 kb on + strand at 3020.443 kb on - strand at 3020.457 kb on - strand at 3020.519 kb on - strand at 3020.577 kb on - strand, within RR42_RS14030 at 3020.578 kb on + strand, within RR42_RS14030 at 3020.579 kb on - strand, within RR42_RS14030 at 3020.677 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.683 kb on + strand, within RR42_RS14030 at 3020.834 kb on - strand, within RR42_RS14030 at 3020.891 kb on - strand, within RR42_RS14030 at 3021.024 kb on + strand, within RR42_RS14030
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,019,643 + -0.5 3,019,644 - -1.6 3,019,672 + -3.6 3,020,443 - +2.4 3,020,457 - +0.6 3,020,519 - +0.7 3,020,577 - RR42_RS14030 0.13 -0.7 3,020,578 + RR42_RS14030 0.13 +0.3 3,020,579 - RR42_RS14030 0.14 -2.3 3,020,677 + RR42_RS14030 0.25 -1.0 3,020,683 + RR42_RS14030 0.25 -0.9 3,020,683 + RR42_RS14030 0.25 -1.9 3,020,834 - RR42_RS14030 0.43 -1.7 3,020,891 - RR42_RS14030 0.49 -0.5 3,021,024 + RR42_RS14030 0.64 -3.4
Or see this region's nucleotide sequence