Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13540 and RR42_RS13545 are separated by 15 nucleotides RR42_RS13545 and RR42_RS13550 are separated by 52 nucleotides RR42_RS13550 and RR42_RS13555 are separated by 2 nucleotides
RR42_RS13540: RR42_RS13540 - acyl-CoA dehydrogenase, at 2,922,637 to 2,923,800
_RS13540
RR42_RS13545: RR42_RS13545 - enoyl-CoA hydratase, at 2,923,816 to 2,924,667
_RS13545
RR42_RS13550: RR42_RS13550 - 3-hydroxyacyl-CoA dehydrogenase, at 2,924,720 to 2,925,520
_RS13550
RR42_RS13555: RR42_RS13555 - anthraniloyl-CoA monooxygenase (EC 1.14.13.40) (from data) , at 2,925,523 to 2,927,913
_RS13555
Position (kb)
2923
2924
2925 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2922.893 kb on + strand, within RR42_RS13540 at 2923.289 kb on + strand, within RR42_RS13540 at 2923.290 kb on - strand, within RR42_RS13540 at 2923.808 kb on + strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.809 kb on - strand at 2923.863 kb on - strand at 2924.656 kb on + strand at 2924.767 kb on + strand at 2924.942 kb on - strand, within RR42_RS13550 at 2925.051 kb on + strand, within RR42_RS13550 at 2925.051 kb on + strand, within RR42_RS13550 at 2925.052 kb on - strand, within RR42_RS13550 at 2925.058 kb on - strand, within RR42_RS13550 at 2925.091 kb on + strand, within RR42_RS13550 at 2925.092 kb on - strand, within RR42_RS13550 at 2925.608 kb on + strand at 2925.609 kb on - strand at 2925.609 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,922,893 + RR42_RS13540 0.22 +0.3 2,923,289 + RR42_RS13540 0.56 +0.2 2,923,290 - RR42_RS13540 0.56 +0.4 2,923,808 + -0.4 2,923,809 - +0.3 2,923,809 - -3.3 2,923,809 - +0.8 2,923,809 - +0.2 2,923,863 - +0.8 2,924,656 + +1.5 2,924,767 + -1.1 2,924,942 - RR42_RS13550 0.28 -1.3 2,925,051 + RR42_RS13550 0.41 +2.3 2,925,051 + RR42_RS13550 0.41 -1.2 2,925,052 - RR42_RS13550 0.41 -2.4 2,925,058 - RR42_RS13550 0.42 -0.0 2,925,091 + RR42_RS13550 0.46 +0.1 2,925,092 - RR42_RS13550 0.46 -0.2 2,925,608 + -1.4 2,925,609 - +0.1 2,925,609 - +0.1
Or see this region's nucleotide sequence