Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07605 and RR42_RS07610 are separated by 76 nucleotides RR42_RS07610 and RR42_RS07615 are separated by 115 nucleotides
RR42_RS07605: RR42_RS07605 - poly-beta-hydroxybutyrate polymerase, at 1,628,303 to 1,630,096
_RS07605
RR42_RS07610: RR42_RS07610 - acetyl-CoA acetyltransferase, at 1,630,173 to 1,631,354
_RS07610
RR42_RS07615: RR42_RS07615 - 3-ketoacyl-ACP reductase, at 1,631,470 to 1,632,210
_RS07615
Position (kb)
1630
1631
1632 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1629.543 kb on - strand, within RR42_RS07605 at 1629.656 kb on - strand, within RR42_RS07605 at 1629.744 kb on - strand, within RR42_RS07605 at 1629.749 kb on + strand, within RR42_RS07605 at 1629.750 kb on - strand, within RR42_RS07605 at 1630.046 kb on + strand at 1630.095 kb on - strand at 1630.144 kb on - strand at 1630.192 kb on + strand at 1630.626 kb on + strand, within RR42_RS07610 at 1630.626 kb on + strand, within RR42_RS07610 at 1630.627 kb on - strand, within RR42_RS07610 at 1630.636 kb on - strand, within RR42_RS07610 at 1630.674 kb on + strand, within RR42_RS07610 at 1630.675 kb on - strand, within RR42_RS07610 at 1631.174 kb on + strand, within RR42_RS07610 at 1631.175 kb on - strand, within RR42_RS07610 at 1631.444 kb on + strand at 1631.444 kb on + strand at 1631.451 kb on + strand at 1631.466 kb on - strand at 1631.466 kb on - strand at 1631.469 kb on + strand at 1631.469 kb on + strand at 1631.469 kb on + strand at 1631.470 kb on - strand at 1631.488 kb on + strand at 1631.488 kb on + strand at 1631.492 kb on + strand at 1631.492 kb on + strand at 1631.926 kb on + strand, within RR42_RS07615 at 1631.926 kb on + strand, within RR42_RS07615 at 1631.927 kb on - strand, within RR42_RS07615 at 1632.324 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,629,543 - RR42_RS07605 0.69 -0.3 1,629,656 - RR42_RS07605 0.75 -1.8 1,629,744 - RR42_RS07605 0.80 -1.3 1,629,749 + RR42_RS07605 0.81 -0.4 1,629,750 - RR42_RS07605 0.81 -2.2 1,630,046 + +4.2 1,630,095 - +0.6 1,630,144 - -4.6 1,630,192 + -3.7 1,630,626 + RR42_RS07610 0.38 -2.0 1,630,626 + RR42_RS07610 0.38 -3.2 1,630,627 - RR42_RS07610 0.38 -4.6 1,630,636 - RR42_RS07610 0.39 -0.1 1,630,674 + RR42_RS07610 0.42 -3.0 1,630,675 - RR42_RS07610 0.42 -3.4 1,631,174 + RR42_RS07610 0.85 -4.2 1,631,175 - RR42_RS07610 0.85 -1.6 1,631,444 + -1.7 1,631,444 + -3.2 1,631,451 + -4.3 1,631,466 - -3.0 1,631,466 - -2.5 1,631,469 + -4.5 1,631,469 + -2.8 1,631,469 + +0.6 1,631,470 - -4.5 1,631,488 + +0.2 1,631,488 + -2.9 1,631,492 + -2.9 1,631,492 + -3.0 1,631,926 + RR42_RS07615 0.62 -2.5 1,631,926 + RR42_RS07615 0.62 -3.3 1,631,927 - RR42_RS07615 0.62 -0.7 1,632,324 + -2.8
Or see this region's nucleotide sequence