Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06425 and RR42_RS06430 are separated by 85 nucleotides RR42_RS06430 and RR42_RS06435 are separated by 21 nucleotides RR42_RS06435 and RR42_RS06440 are separated by 86 nucleotides RR42_RS06440 and RR42_RS06445 are separated by 71 nucleotides
RR42_RS06425: RR42_RS06425 - anhydrase, at 1,407,671 to 1,408,213
_RS06425
RR42_RS06430: RR42_RS06430 - hypothetical protein, at 1,408,299 to 1,408,529
_RS06430
RR42_RS06435: RR42_RS06435 - 2-hydroxyacid dehydrogenase, at 1,408,551 to 1,409,972
_RS06435
RR42_RS06440: RR42_RS06440 - membrane protein, at 1,410,059 to 1,410,433
_RS06440
RR42_RS06445: RR42_RS06445 - NAD(P)H-quinone oxidoreductase, at 1,410,505 to 1,411,110
_RS06445
Position (kb)
1408
1409
1410 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1407.668 kb on - strand at 1407.756 kb on + strand, within RR42_RS06425 at 1407.963 kb on + strand, within RR42_RS06425 at 1407.964 kb on - strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.153 kb on + strand, within RR42_RS06425 at 1408.154 kb on - strand, within RR42_RS06425 at 1408.167 kb on - strand at 1408.239 kb on + strand at 1408.355 kb on + strand, within RR42_RS06430 at 1408.356 kb on - strand, within RR42_RS06430 at 1408.795 kb on + strand, within RR42_RS06435 at 1408.795 kb on + strand, within RR42_RS06435 at 1408.795 kb on + strand, within RR42_RS06435 at 1409.389 kb on + strand, within RR42_RS06435 at 1409.389 kb on + strand, within RR42_RS06435 at 1409.390 kb on - strand, within RR42_RS06435 at 1409.390 kb on - strand, within RR42_RS06435 at 1409.448 kb on + strand, within RR42_RS06435 at 1409.480 kb on + strand, within RR42_RS06435 at 1409.736 kb on + strand, within RR42_RS06435 at 1409.736 kb on + strand, within RR42_RS06435 at 1409.854 kb on + strand at 1409.879 kb on - strand at 1410.105 kb on + strand, within RR42_RS06440 at 1410.105 kb on + strand, within RR42_RS06440 at 1410.106 kb on - strand, within RR42_RS06440 at 1410.117 kb on + strand, within RR42_RS06440 at 1410.261 kb on + strand, within RR42_RS06440 at 1410.681 kb on - strand, within RR42_RS06445 at 1410.845 kb on + strand, within RR42_RS06445 at 1410.846 kb on - strand, within RR42_RS06445 at 1410.846 kb on - strand, within RR42_RS06445
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,407,668 - -2.3 1,407,756 + RR42_RS06425 0.16 -3.1 1,407,963 + RR42_RS06425 0.54 -3.2 1,407,964 - RR42_RS06425 0.54 +0.5 1,408,088 + RR42_RS06425 0.77 -3.1 1,408,088 + RR42_RS06425 0.77 -1.4 1,408,089 - RR42_RS06425 0.77 -0.1 1,408,089 - RR42_RS06425 0.77 +1.1 1,408,089 - RR42_RS06425 0.77 +1.0 1,408,089 - RR42_RS06425 0.77 +0.2 1,408,153 + RR42_RS06425 0.89 -0.2 1,408,154 - RR42_RS06425 0.89 +0.5 1,408,167 - -3.5 1,408,239 + -0.1 1,408,355 + RR42_RS06430 0.24 +1.5 1,408,356 - RR42_RS06430 0.25 +0.0 1,408,795 + RR42_RS06435 0.17 +0.6 1,408,795 + RR42_RS06435 0.17 +0.7 1,408,795 + RR42_RS06435 0.17 +2.0 1,409,389 + RR42_RS06435 0.59 +0.9 1,409,389 + RR42_RS06435 0.59 +1.2 1,409,390 - RR42_RS06435 0.59 -2.5 1,409,390 - RR42_RS06435 0.59 -2.5 1,409,448 + RR42_RS06435 0.63 -0.0 1,409,480 + RR42_RS06435 0.65 +1.3 1,409,736 + RR42_RS06435 0.83 +0.8 1,409,736 + RR42_RS06435 0.83 -1.6 1,409,854 + +0.4 1,409,879 - +0.9 1,410,105 + RR42_RS06440 0.12 +0.3 1,410,105 + RR42_RS06440 0.12 -2.5 1,410,106 - RR42_RS06440 0.13 +0.2 1,410,117 + RR42_RS06440 0.15 +0.5 1,410,261 + RR42_RS06440 0.54 +0.5 1,410,681 - RR42_RS06445 0.29 +2.9 1,410,845 + RR42_RS06445 0.56 +1.1 1,410,846 - RR42_RS06445 0.56 +0.4 1,410,846 - RR42_RS06445 0.56 -3.8
Or see this region's nucleotide sequence