Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05810 and RR42_RS05815 overlap by 4 nucleotides RR42_RS05815 and RR42_RS05820 overlap by 4 nucleotides RR42_RS05820 and RR42_RS05825 are separated by 82 nucleotides
RR42_RS05810: RR42_RS05810 - RNA polymerase sigma factor, at 1,278,101 to 1,278,661
_RS05810
RR42_RS05815: RR42_RS05815 - hypothetical protein, at 1,278,658 to 1,279,056
_RS05815
RR42_RS05820: RR42_RS05820 - hypothetical protein, at 1,279,053 to 1,279,538
_RS05820
RR42_RS05825: RR42_RS05825 - hypothetical protein, at 1,279,621 to 1,281,006
_RS05825
Position (kb)
1278
1279
1280 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1277.897 kb on - strand at 1277.897 kb on - strand at 1277.989 kb on + strand at 1277.990 kb on - strand at 1277.992 kb on + strand at 1277.992 kb on + strand at 1277.993 kb on - strand at 1277.993 kb on - strand at 1277.993 kb on - strand at 1277.993 kb on - strand at 1277.999 kb on - strand at 1278.096 kb on - strand at 1278.211 kb on + strand, within RR42_RS05810 at 1278.354 kb on - strand, within RR42_RS05810 at 1278.522 kb on - strand, within RR42_RS05810 at 1278.694 kb on + strand at 1278.694 kb on + strand at 1278.694 kb on + strand at 1278.695 kb on - strand at 1278.695 kb on - strand at 1278.695 kb on - strand at 1278.695 kb on - strand at 1279.064 kb on + strand at 1279.065 kb on - strand at 1279.571 kb on - strand at 1279.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,277,897 - +0.7 1,277,897 - -0.2 1,277,989 + -0.0 1,277,990 - +1.7 1,277,992 + -1.2 1,277,992 + +0.4 1,277,993 - +2.9 1,277,993 - +0.8 1,277,993 - +0.4 1,277,993 - -2.5 1,277,999 - +0.8 1,278,096 - +0.8 1,278,211 + RR42_RS05810 0.20 -2.5 1,278,354 - RR42_RS05810 0.45 -1.5 1,278,522 - RR42_RS05810 0.75 -0.8 1,278,694 + -1.1 1,278,694 + -0.9 1,278,694 + +2.2 1,278,695 - +0.9 1,278,695 - -3.0 1,278,695 - -1.6 1,278,695 - +1.1 1,279,064 + -2.1 1,279,065 - +2.0 1,279,571 - +1.5 1,279,742 - +1.5
Or see this region's nucleotide sequence