Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05605 and RR42_RS05610 are separated by 83 nucleotides RR42_RS05610 and RR42_RS05615 are separated by 59 nucleotides RR42_RS05615 and RR42_RS05620 are separated by 9 nucleotides RR42_RS05620 and RR42_RS05625 are separated by 181 nucleotides
RR42_RS05605: RR42_RS05605 - dehydratase, at 1,234,386 to 1,234,835
_RS05605
RR42_RS05610: RR42_RS05610 - dehydratase, at 1,234,919 to 1,235,398
_RS05610
RR42_RS05615: RR42_RS05615 - MFS transporter, at 1,235,458 to 1,236,759
_RS05615
RR42_RS05620: RR42_RS05620 - esterase, at 1,236,769 to 1,237,527
_RS05620
RR42_RS05625: RR42_RS05625 - 4-hydroxyacetophenone monooxygenase, at 1,237,709 to 1,239,196
_RS05625
Position (kb) 1235
1236
1237 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 1234.485 kb on + strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.486 kb on - strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.536 kb on + strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.537 kb on - strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.668 kb on + strand, within RR42_RS05605 at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.815 kb on + strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.816 kb on - strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.884 kb on + strand at 1234.885 kb on - strand at 1234.885 kb on - strand at 1234.893 kb on + strand at 1234.893 kb on + strand at 1234.894 kb on - strand at 1234.894 kb on - strand at 1234.902 kb on + strand at 1234.902 kb on + strand at 1234.925 kb on - strand at 1235.187 kb on - strand, within RR42_RS05610 at 1235.209 kb on + strand, within RR42_RS05610 at 1235.397 kb on - strand at 1235.397 kb on - strand at 1235.623 kb on + strand, within RR42_RS05615 at 1235.835 kb on - strand, within RR42_RS05615 at 1235.872 kb on + strand, within RR42_RS05615 at 1235.872 kb on + strand, within RR42_RS05615 at 1235.872 kb on + strand, within RR42_RS05615 at 1236.063 kb on - strand, within RR42_RS05615 at 1236.123 kb on - strand, within RR42_RS05615 at 1236.271 kb on + strand, within RR42_RS05615 at 1236.272 kb on - strand, within RR42_RS05615 at 1236.292 kb on + strand, within RR42_RS05615 at 1236.313 kb on + strand, within RR42_RS05615 at 1236.314 kb on - strand, within RR42_RS05615 at 1236.740 kb on + strand at 1236.740 kb on + strand at 1236.740 kb on + strand at 1236.741 kb on - strand at 1236.753 kb on + strand at 1236.902 kb on + strand, within RR42_RS05620 at 1236.902 kb on + strand, within RR42_RS05620 at 1237.323 kb on - strand, within RR42_RS05620 at 1237.711 kb on - strand at 1237.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,234,485 + RR42_RS05605 0.22 -0.5 1,234,486 - RR42_RS05605 0.22 -0.8 1,234,486 - RR42_RS05605 0.22 -1.7 1,234,486 - RR42_RS05605 0.22 -1.1 1,234,486 - RR42_RS05605 0.22 +1.1 1,234,536 + RR42_RS05605 0.33 +0.3 1,234,536 + RR42_RS05605 0.33 +0.1 1,234,537 - RR42_RS05605 0.34 -3.1 1,234,537 - RR42_RS05605 0.34 -0.2 1,234,668 + RR42_RS05605 0.63 +1.8 1,234,668 + RR42_RS05605 0.63 +0.4 1,234,815 + +1.1 1,234,815 + -3.9 1,234,815 + -0.5 1,234,816 - -2.2 1,234,816 - -1.1 1,234,816 - +0.8 1,234,816 - +1.1 1,234,816 - -1.6 1,234,884 + +2.9 1,234,884 + +1.5 1,234,884 + -1.1 1,234,885 - +0.6 1,234,885 - -0.6 1,234,893 + +0.5 1,234,893 + -0.1 1,234,894 - -3.8 1,234,894 - -1.9 1,234,902 + -0.2 1,234,902 + +2.5 1,234,925 - -2.0 1,235,187 - RR42_RS05610 0.56 -4.8 1,235,209 + RR42_RS05610 0.60 +1.0 1,235,397 - +1.1 1,235,397 - -1.7 1,235,623 + RR42_RS05615 0.13 -3.4 1,235,835 - RR42_RS05615 0.29 -0.9 1,235,872 + RR42_RS05615 0.32 -0.2 1,235,872 + RR42_RS05615 0.32 -3.5 1,235,872 + RR42_RS05615 0.32 -0.1 1,236,063 - RR42_RS05615 0.46 +0.5 1,236,123 - RR42_RS05615 0.51 +0.1 1,236,271 + RR42_RS05615 0.62 +0.1 1,236,272 - RR42_RS05615 0.63 +0.9 1,236,292 + RR42_RS05615 0.64 -1.1 1,236,313 + RR42_RS05615 0.66 +2.6 1,236,314 - RR42_RS05615 0.66 -4.2 1,236,740 + +0.1 1,236,740 + -0.6 1,236,740 + +2.2 1,236,741 - -0.3 1,236,753 + -0.9 1,236,902 + RR42_RS05620 0.18 -1.1 1,236,902 + RR42_RS05620 0.18 -3.9 1,237,323 - RR42_RS05620 0.73 +0.3 1,237,711 - -2.6 1,237,711 - -2.3
Or see this region's nucleotide sequence