Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05100 and RR42_RS05105 are separated by 2 nucleotides RR42_RS05105 and RR42_RS05110 are separated by 45 nucleotides RR42_RS05110 and RR42_RS05115 are separated by 153 nucleotides
RR42_RS05100: RR42_RS05100 - acetaldehyde dehydrogenase, at 1,125,769 to 1,126,734
_RS05100
RR42_RS05105: RR42_RS05105 - 2-keto-4-pentenoate hydratase, at 1,126,737 to 1,127,528
_RS05105
RR42_RS05110: RR42_RS05110 - betaine-aldehyde dehydrogenase, at 1,127,574 to 1,129,043
_RS05110
RR42_RS05115: RR42_RS05115 - LysR family transcriptional regulator, at 1,129,197 to 1,130,165
_RS05115
Position (kb) 1127
1128
1129
1130 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1127.608 kb on + strand at 1127.608 kb on + strand at 1127.788 kb on + strand, within RR42_RS05110 at 1127.924 kb on - strand, within RR42_RS05110 at 1128.057 kb on - strand, within RR42_RS05110 at 1128.619 kb on + strand, within RR42_RS05110 at 1128.620 kb on - strand, within RR42_RS05110 at 1128.658 kb on + strand, within RR42_RS05110 at 1128.660 kb on + strand, within RR42_RS05110 at 1128.660 kb on + strand, within RR42_RS05110 at 1128.661 kb on - strand, within RR42_RS05110 at 1128.847 kb on - strand, within RR42_RS05110 at 1129.097 kb on + strand at 1129.098 kb on - strand at 1129.098 kb on - strand at 1129.098 kb on - strand at 1129.100 kb on - strand at 1129.100 kb on - strand at 1129.123 kb on + strand at 1129.629 kb on - strand, within RR42_RS05115 at 1129.888 kb on + strand, within RR42_RS05115 at 1129.889 kb on - strand, within RR42_RS05115
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,127,608 + -0.2 1,127,608 + -1.8 1,127,788 + RR42_RS05110 0.15 -0.3 1,127,924 - RR42_RS05110 0.24 -0.8 1,128,057 - RR42_RS05110 0.33 -0.9 1,128,619 + RR42_RS05110 0.71 +1.1 1,128,620 - RR42_RS05110 0.71 -2.5 1,128,658 + RR42_RS05110 0.74 +0.4 1,128,660 + RR42_RS05110 0.74 -3.3 1,128,660 + RR42_RS05110 0.74 -0.3 1,128,661 - RR42_RS05110 0.74 +1.7 1,128,847 - RR42_RS05110 0.87 -1.6 1,129,097 + -1.4 1,129,098 - +0.1 1,129,098 - +0.3 1,129,098 - +0.5 1,129,100 - -2.4 1,129,100 - -0.6 1,129,123 + -3.1 1,129,629 - RR42_RS05115 0.45 -3.5 1,129,888 + RR42_RS05115 0.71 -0.5 1,129,889 - RR42_RS05115 0.71 +0.6
Or see this region's nucleotide sequence