Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04715 and RR42_RS04720 are separated by 15 nucleotides RR42_RS04720 and RR42_RS04725 are separated by 10 nucleotides RR42_RS04725 and RR42_RS04730 are separated by 222 nucleotides
RR42_RS04715: RR42_RS04715 - ribosome silencing factor RsfS, at 1,049,937 to 1,050,695
_RS04715
RR42_RS04720: RR42_RS04720 - nicotinate-nucleotide adenylyltransferase, at 1,050,711 to 1,051,481
_RS04720
RR42_RS04725: RR42_RS04725 - coproporphyrinogen III oxidase, at 1,051,492 to 1,052,403
_RS04725
RR42_RS04730: RR42_RS04730 - phosphoribosylamine--glycine ligase, at 1,052,626 to 1,053,894
_RS04730
Position (kb)
1051
1052
1053 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1050.622 kb on + strand at 1050.623 kb on - strand at 1050.623 kb on - strand at 1050.689 kb on + strand at 1050.690 kb on - strand at 1052.407 kb on - strand at 1052.407 kb on - strand at 1052.490 kb on + strand at 1052.490 kb on + strand at 1052.490 kb on + strand at 1052.490 kb on + strand at 1052.491 kb on - strand at 1052.491 kb on - strand at 1052.524 kb on - strand at 1052.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,050,622 + -3.0 1,050,623 - +0.8 1,050,623 - -2.1 1,050,689 + -3.8 1,050,690 - -2.1 1,052,407 - +1.2 1,052,407 - -3.0 1,052,490 + -2.7 1,052,490 + -0.3 1,052,490 + +2.1 1,052,490 + -0.5 1,052,491 - -1.5 1,052,491 - +0.3 1,052,524 - -2.0 1,052,628 - +0.3
Or see this region's nucleotide sequence