Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04710 and RR42_RS04715 are separated by 23 nucleotides RR42_RS04715 and RR42_RS04720 are separated by 15 nucleotides RR42_RS04720 and RR42_RS04725 are separated by 10 nucleotides
RR42_RS04710: RR42_RS04710 - 50S rRNA methyltransferase, at 1,049,443 to 1,049,913
_RS04710
RR42_RS04715: RR42_RS04715 - ribosome silencing factor RsfS, at 1,049,937 to 1,050,695
_RS04715
RR42_RS04720: RR42_RS04720 - nicotinate-nucleotide adenylyltransferase, at 1,050,711 to 1,051,481
_RS04720
RR42_RS04725: RR42_RS04725 - coproporphyrinogen III oxidase, at 1,051,492 to 1,052,403
_RS04725
Position (kb) 1050
1051
1052 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1049.772 kb on + strand, within RR42_RS04710 at 1049.772 kb on + strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1049.773 kb on - strand, within RR42_RS04710 at 1050.173 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.403 kb on + strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.404 kb on - strand, within RR42_RS04715 at 1050.406 kb on + strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.407 kb on - strand, within RR42_RS04715 at 1050.622 kb on + strand at 1050.623 kb on - strand at 1050.623 kb on - strand at 1050.689 kb on + strand at 1050.690 kb on - strand at 1052.407 kb on - strand at 1052.407 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,049,772 + RR42_RS04710 0.70 -0.1 1,049,772 + RR42_RS04710 0.70 -0.4 1,049,773 - RR42_RS04710 0.70 +2.0 1,049,773 - RR42_RS04710 0.70 -2.6 1,050,173 + RR42_RS04715 0.31 -2.3 1,050,403 + RR42_RS04715 0.61 +0.0 1,050,403 + RR42_RS04715 0.61 -0.1 1,050,404 - RR42_RS04715 0.62 -0.4 1,050,404 - RR42_RS04715 0.62 -1.4 1,050,406 + RR42_RS04715 0.62 -2.6 1,050,407 - RR42_RS04715 0.62 -0.9 1,050,407 - RR42_RS04715 0.62 -0.6 1,050,622 + -3.0 1,050,623 - +0.8 1,050,623 - -2.1 1,050,689 + -3.8 1,050,690 - -2.1 1,052,407 - +1.2 1,052,407 - -3.0
Or see this region's nucleotide sequence