Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02015 and RR42_RS02020 are separated by 97 nucleotides RR42_RS02020 and RR42_RS02025 are separated by 59 nucleotides RR42_RS02025 and RR42_RS02030 overlap by 4 nucleotides RR42_RS02030 and RR42_RS02035 are separated by 119 nucleotides
RR42_RS02015: RR42_RS02015 - ribose-phosphate pyrophosphokinase, at 449,464 to 450,417
_RS02015
RR42_RS02020: RR42_RS02020 - tRNA-Gln, at 450,515 to 450,591
_RS02020
RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529
_RS02025
RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146
_RS02030
RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149
_RS02035
Position (kb) 450
451
452 Strain fitness (log2 ratio) -2
-1
0
1
2 at 450.513 kb on - strand at 450.513 kb on - strand at 450.513 kb on - strand at 451.820 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.267 kb on + strand at 452.267 kb on + strand at 452.296 kb on - strand at 452.296 kb on - strand at 452.298 kb on - strand at 452.339 kb on - strand at 452.339 kb on - strand at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 450,513 - +0.3 450,513 - -1.3 450,513 - -1.5 451,820 - RR42_RS02030 0.47 -2.0 452,002 - RR42_RS02030 0.77 +1.1 452,002 - RR42_RS02030 0.77 +1.4 452,267 + +1.8 452,267 + +2.4 452,296 - -1.8 452,296 - -1.9 452,298 - +0.2 452,339 - +1.7 452,339 - -2.0 452,523 - RR42_RS02035 0.14 +0.7 452,523 - RR42_RS02035 0.14 +0.5 452,523 - RR42_RS02035 0.14 +1.0 452,523 - RR42_RS02035 0.14 -0.3
Or see this region's nucleotide sequence