Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01385 and RR42_RS01390 are separated by 22 nucleotides RR42_RS01390 and RR42_RS01395 are separated by 31 nucleotides RR42_RS01395 and RR42_RS01400 are separated by 22 nucleotides RR42_RS01400 and RR42_RS01405 are separated by 153 nucleotides
RR42_RS01385: RR42_RS01385 - 5,10-methylenetetrahydrofolate reductase, at 313,461 to 314,291
_RS01385
RR42_RS01390: RR42_RS01390 - competence protein, at 314,314 to 314,676
_RS01390
RR42_RS01395: RR42_RS01395 - twitching motility protein PilT, at 314,708 to 315,475
_RS01395
RR42_RS01400: RR42_RS01400 - 5-formyltetrahydrofolate cyclo-ligase, at 315,498 to 316,049
_RS01400
RR42_RS01405: RR42_RS01405 - lytic transglycosylase, at 316,203 to 318,155
_RS01405
Position (kb) 314
315
316 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 313.717 kb on - strand, within RR42_RS01385 at 313.875 kb on + strand, within RR42_RS01385 at 313.962 kb on + strand, within RR42_RS01385 at 314.235 kb on + strand at 314.237 kb on + strand at 314.237 kb on + strand at 314.289 kb on + strand at 314.290 kb on - strand at 314.428 kb on + strand, within RR42_RS01390 at 314.428 kb on + strand, within RR42_RS01390 at 314.431 kb on + strand, within RR42_RS01390 at 314.431 kb on + strand, within RR42_RS01390 at 314.432 kb on - strand, within RR42_RS01390 at 314.432 kb on - strand, within RR42_RS01390 at 314.474 kb on - strand, within RR42_RS01390 at 314.572 kb on + strand, within RR42_RS01390 at 314.573 kb on - strand, within RR42_RS01390 at 314.697 kb on + strand at 314.697 kb on + strand at 314.751 kb on - strand at 314.945 kb on + strand, within RR42_RS01395 at 315.083 kb on + strand, within RR42_RS01395 at 315.084 kb on - strand, within RR42_RS01395 at 315.095 kb on + strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.096 kb on - strand, within RR42_RS01395 at 315.197 kb on + strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395 at 315.198 kb on - strand, within RR42_RS01395 at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.430 kb on + strand at 315.431 kb on - strand at 315.431 kb on - strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.473 kb on + strand at 315.474 kb on - strand at 315.644 kb on - strand, within RR42_RS01400 at 315.674 kb on + strand, within RR42_RS01400 at 315.675 kb on - strand, within RR42_RS01400 at 315.708 kb on + strand, within RR42_RS01400 at 315.709 kb on - strand, within RR42_RS01400 at 315.754 kb on + strand, within RR42_RS01400 at 315.755 kb on - strand, within RR42_RS01400 at 315.913 kb on + strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 315.914 kb on - strand, within RR42_RS01400 at 316.119 kb on + strand at 316.120 kb on - strand at 316.216 kb on + strand at 316.216 kb on + strand at 316.217 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.219 kb on - strand at 316.282 kb on + strand at 316.283 kb on - strand at 316.431 kb on + strand, within RR42_RS01405 at 316.446 kb on + strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.447 kb on - strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405 at 316.455 kb on + strand, within RR42_RS01405
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 313,717 - RR42_RS01385 0.31 -3.7 313,875 + RR42_RS01385 0.50 -2.2 313,962 + RR42_RS01385 0.60 -4.5 314,235 + -2.2 314,237 + -3.6 314,237 + -4.6 314,289 + +1.1 314,290 - +0.0 314,428 + RR42_RS01390 0.31 -0.6 314,428 + RR42_RS01390 0.31 -3.9 314,431 + RR42_RS01390 0.32 -1.3 314,431 + RR42_RS01390 0.32 +0.9 314,432 - RR42_RS01390 0.33 -0.4 314,432 - RR42_RS01390 0.33 -2.9 314,474 - RR42_RS01390 0.44 -3.2 314,572 + RR42_RS01390 0.71 -3.7 314,573 - RR42_RS01390 0.71 -3.6 314,697 + +0.3 314,697 + +0.2 314,751 - -1.4 314,945 + RR42_RS01395 0.31 -3.5 315,083 + RR42_RS01395 0.49 +0.6 315,084 - RR42_RS01395 0.49 -3.1 315,095 + RR42_RS01395 0.50 -0.2 315,096 - RR42_RS01395 0.51 +1.5 315,096 - RR42_RS01395 0.51 +1.6 315,197 + RR42_RS01395 0.64 +0.8 315,198 - RR42_RS01395 0.64 +1.4 315,198 - RR42_RS01395 0.64 -3.4 315,430 + -2.1 315,430 + +1.4 315,430 + -1.5 315,430 + -0.6 315,431 - +1.0 315,431 - +1.0 315,473 + -0.7 315,473 + -1.4 315,473 + -0.7 315,473 + -2.9 315,474 - -2.7 315,644 - RR42_RS01400 0.26 +0.7 315,674 + RR42_RS01400 0.32 +0.3 315,675 - RR42_RS01400 0.32 +0.7 315,708 + RR42_RS01400 0.38 +1.8 315,709 - RR42_RS01400 0.38 +0.0 315,754 + RR42_RS01400 0.46 -0.7 315,755 - RR42_RS01400 0.47 -0.2 315,913 + RR42_RS01400 0.75 +1.3 315,914 - RR42_RS01400 0.75 -0.7 315,914 - RR42_RS01400 0.75 +0.6 316,119 + +0.4 316,120 - +2.1 316,216 + +0.1 316,216 + +1.2 316,217 - +2.3 316,219 - +1.6 316,219 - +0.3 316,219 - +0.6 316,282 + +2.0 316,283 - +0.3 316,431 + RR42_RS01405 0.12 +1.1 316,446 + RR42_RS01405 0.12 -0.9 316,447 - RR42_RS01405 0.12 +1.3 316,447 - RR42_RS01405 0.12 -1.4 316,447 - RR42_RS01405 0.12 +1.2 316,447 - RR42_RS01405 0.12 +2.6 316,455 + RR42_RS01405 0.13 -0.4 316,455 + RR42_RS01405 0.13 -3.9
Or see this region's nucleotide sequence