Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00895 and RR42_RS00900 are separated by 119 nucleotides RR42_RS00900 and RR42_RS00905 overlap by 4 nucleotides RR42_RS00905 and RR42_RS00910 overlap by 4 nucleotides RR42_RS00910 and RR42_RS00915 are separated by 8 nucleotides
RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947
_RS00895
RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768
_RS00900
RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454
_RS00905
RR42_RS00910: RR42_RS00910 - hypothetical protein, at 218,451 to 218,876
_RS00910
RR42_RS00915: RR42_RS00915 - glutathione S-transferase, at 218,885 to 219,559
_RS00915
Position (kb)
217
218
219 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 216.774 kb on + strand, within RR42_RS00895 at 216.774 kb on + strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.775 kb on - strand, within RR42_RS00895 at 216.895 kb on - strand at 216.922 kb on + strand at 216.923 kb on - strand at 216.923 kb on - strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.088 kb on + strand at 217.089 kb on - strand at 217.154 kb on + strand, within RR42_RS00900 at 217.250 kb on + strand, within RR42_RS00900 at 217.292 kb on - strand, within RR42_RS00900 at 217.299 kb on - strand, within RR42_RS00900 at 217.730 kb on - strand at 217.899 kb on + strand, within RR42_RS00905 at 217.900 kb on - strand, within RR42_RS00905 at 218.202 kb on + strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.203 kb on - strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.251 kb on + strand, within RR42_RS00905 at 218.252 kb on - strand, within RR42_RS00905 at 218.310 kb on + strand, within RR42_RS00905 at 218.425 kb on - strand at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.592 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.639 kb on + strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.640 kb on - strand, within RR42_RS00910 at 218.752 kb on - strand, within RR42_RS00910 at 218.800 kb on + strand, within RR42_RS00910 at 218.801 kb on - strand, within RR42_RS00910 at 218.841 kb on + strand at 218.912 kb on + strand at 218.912 kb on + strand at 218.921 kb on + strand at 218.921 kb on + strand at 218.922 kb on - strand at 218.922 kb on - strand at 218.922 kb on - strand at 218.948 kb on + strand at 218.948 kb on + strand at 218.949 kb on - strand at 218.949 kb on - strand at 218.949 kb on - strand at 219.161 kb on + strand, within RR42_RS00915 at 219.162 kb on - strand, within RR42_RS00915 at 219.162 kb on - strand, within RR42_RS00915 at 219.162 kb on - strand, within RR42_RS00915 at 219.162 kb on - strand, within RR42_RS00915 at 219.236 kb on + strand, within RR42_RS00915 at 219.236 kb on + strand, within RR42_RS00915 at 219.248 kb on + strand, within RR42_RS00915 at 219.249 kb on - strand, within RR42_RS00915 at 219.419 kb on + strand, within RR42_RS00915
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 216,774 + RR42_RS00895 0.85 +0.4 216,774 + RR42_RS00895 0.85 -1.8 216,775 - RR42_RS00895 0.85 +2.7 216,775 - RR42_RS00895 0.85 +0.2 216,895 - -1.4 216,922 + +2.1 216,923 - +1.9 216,923 - +0.6 217,088 + +1.0 217,088 + +0.7 217,088 + +1.7 217,089 - -2.5 217,154 + RR42_RS00900 0.12 -1.6 217,250 + RR42_RS00900 0.26 -4.0 217,292 - RR42_RS00900 0.32 +0.6 217,299 - RR42_RS00900 0.33 -2.8 217,730 - +0.2 217,899 + RR42_RS00905 0.19 +1.2 217,900 - RR42_RS00905 0.20 +0.2 218,202 + RR42_RS00905 0.63 +0.1 218,203 - RR42_RS00905 0.63 +0.6 218,203 - RR42_RS00905 0.63 +0.6 218,251 + RR42_RS00905 0.70 +1.0 218,251 + RR42_RS00905 0.70 -0.5 218,251 + RR42_RS00905 0.70 +1.6 218,252 - RR42_RS00905 0.71 +4.9 218,310 + RR42_RS00905 0.79 -1.3 218,425 - -0.8 218,592 + RR42_RS00910 0.33 +0.7 218,592 + RR42_RS00910 0.33 -3.0 218,592 + RR42_RS00910 0.33 +2.4 218,639 + RR42_RS00910 0.44 +0.3 218,639 + RR42_RS00910 0.44 +1.9 218,640 - RR42_RS00910 0.44 -2.9 218,640 - RR42_RS00910 0.44 -0.7 218,752 - RR42_RS00910 0.71 -4.2 218,800 + RR42_RS00910 0.82 -3.5 218,801 - RR42_RS00910 0.82 +2.4 218,841 + -2.1 218,912 + +2.0 218,912 + +0.9 218,921 + +2.0 218,921 + -0.4 218,922 - -0.8 218,922 - +1.3 218,922 - +1.3 218,948 + -0.1 218,948 + +0.0 218,949 - -2.7 218,949 - -1.3 218,949 - +3.4 219,161 + RR42_RS00915 0.41 -1.1 219,162 - RR42_RS00915 0.41 +0.2 219,162 - RR42_RS00915 0.41 -2.8 219,162 - RR42_RS00915 0.41 -1.5 219,162 - RR42_RS00915 0.41 -2.1 219,236 + RR42_RS00915 0.52 +1.1 219,236 + RR42_RS00915 0.52 -0.5 219,248 + RR42_RS00915 0.54 +0.2 219,249 - RR42_RS00915 0.54 +1.2 219,419 + RR42_RS00915 0.79 +1.9
Or see this region's nucleotide sequence