Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_899 and Pf1N1B4_900 are separated by 8 nucleotides Pf1N1B4_900 and Pf1N1B4_901 are separated by 129 nucleotides Pf1N1B4_901 and Pf1N1B4_902 overlap by 24 nucleotides
Pf1N1B4_899 - DNA polymerase III chi subunit (EC 2.7.7.7), at 1,026,586 to 1,027,014
_899
Pf1N1B4_900 - FIG00957284: hypothetical protein, at 1,027,023 to 1,027,412
_900
Pf1N1B4_901 - hypothetical protein, at 1,027,542 to 1,027,658
_901
Pf1N1B4_902 - Valyl-tRNA synthetase (EC 6.1.1.9), at 1,027,635 to 1,030,481
_902
Position (kb)
1027
1028 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.604 kb on + strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.605 kb on - strand at 1026.607 kb on - strand at 1026.691 kb on + strand, within Pf1N1B4_899 at 1026.691 kb on + strand, within Pf1N1B4_899 at 1026.702 kb on + strand at 1026.879 kb on - strand, within Pf1N1B4_899 at 1027.001 kb on + strand at 1027.405 kb on - strand at 1027.410 kb on + strand at 1027.410 kb on + strand at 1027.411 kb on - strand at 1027.411 kb on - strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.425 kb on + strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.426 kb on - strand at 1027.459 kb on + strand at 1027.459 kb on + strand at 1027.472 kb on + strand at 1027.586 kb on + strand, within Pf1N1B4_901 at 1027.588 kb on + strand, within Pf1N1B4_901 at 1027.588 kb on + strand, within Pf1N1B4_901 at 1027.588 kb on + strand, within Pf1N1B4_901
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,026,604 + +0.8 1,026,604 + -0.3 1,026,604 + +0.1 1,026,604 + -0.5 1,026,604 + +0.1 1,026,604 + +0.1 1,026,605 - +1.4 1,026,605 - +0.1 1,026,605 - +2.1 1,026,605 - -1.5 1,026,605 - -1.9 1,026,605 - -0.3 1,026,605 - -1.5 1,026,605 - -1.9 1,026,605 - +0.7 1,026,605 - -0.7 1,026,605 - -0.5 1,026,605 - -0.7 1,026,607 - -0.8 1,026,691 + Pf1N1B4_899 0.24 -0.7 1,026,691 + Pf1N1B4_899 0.24 +1.4 1,026,702 + +0.1 1,026,879 - Pf1N1B4_899 0.68 -2.7 1,027,001 + -2.5 1,027,405 - +0.3 1,027,410 + -1.3 1,027,410 + +1.2 1,027,411 - -0.9 1,027,411 - -0.3 1,027,425 + -0.9 1,027,425 + +0.7 1,027,425 + +0.1 1,027,425 + -0.9 1,027,425 + +0.3 1,027,425 + -1.5 1,027,425 + +1.1 1,027,425 + +0.3 1,027,426 - -0.9 1,027,426 - -2.9 1,027,426 - -0.5 1,027,426 - +1.1 1,027,426 - +0.1 1,027,426 - +1.1 1,027,426 - +1.1 1,027,426 - +0.1 1,027,426 - +0.1 1,027,426 - +0.3 1,027,459 + -0.4 1,027,459 + +1.1 1,027,472 + +0.8 1,027,586 + Pf1N1B4_901 0.38 -0.4 1,027,588 + Pf1N1B4_901 0.39 -1.7 1,027,588 + Pf1N1B4_901 0.39 +0.1 1,027,588 + Pf1N1B4_901 0.39 -1.9
Or see this region's nucleotide sequence