Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4871 and Pf1N1B4_4872 are separated by 80 nucleotides Pf1N1B4_4872 and Pf1N1B4_4873 are separated by 37 nucleotides Pf1N1B4_4873 and Pf1N1B4_4874 are separated by 258 nucleotides Pf1N1B4_4874 and Pf1N1B4_4875 are separated by 274 nucleotides
Pf1N1B4_4871 - Aldo-keto reductase, at 5,391,641 to 5,391,895
_4871
Pf1N1B4_4872 - Short-chain dehydrogenase/reductase SDR, at 5,391,976 to 5,392,818
_4872
Pf1N1B4_4873 - clavaldehyde dehydrogenase, at 5,392,856 to 5,393,617
_4873
Pf1N1B4_4874 - hypothetical protein, at 5,393,876 to 5,394,127
_4874
Pf1N1B4_4875 - Msl4750 protein, at 5,394,402 to 5,394,779
_4875
Position (kb)
5392
5393
5394 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.947 kb on + strand at 5391.948 kb on - strand at 5391.951 kb on + strand at 5392.028 kb on + strand at 5392.028 kb on + strand at 5392.490 kb on + strand, within Pf1N1B4_4872 at 5392.555 kb on + strand, within Pf1N1B4_4872 at 5392.556 kb on - strand, within Pf1N1B4_4872 at 5392.579 kb on + strand, within Pf1N1B4_4872 at 5392.580 kb on - strand, within Pf1N1B4_4872 at 5392.782 kb on + strand at 5392.782 kb on + strand at 5393.002 kb on - strand, within Pf1N1B4_4873 at 5393.153 kb on - strand, within Pf1N1B4_4873 at 5393.216 kb on + strand at 5393.216 kb on + strand at 5393.216 kb on + strand, within Pf1N1B4_4873 at 5393.217 kb on - strand, within Pf1N1B4_4873 at 5393.316 kb on + strand, within Pf1N1B4_4873 at 5393.330 kb on + strand at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.331 kb on - strand, within Pf1N1B4_4873 at 5393.340 kb on - strand, within Pf1N1B4_4873 at 5393.498 kb on + strand, within Pf1N1B4_4873 at 5393.550 kb on + strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.550 kb on - strand at 5393.616 kb on - strand at 5393.679 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.694 kb on + strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.695 kb on - strand at 5393.794 kb on - strand at 5393.900 kb on - strand at 5393.927 kb on - strand, within Pf1N1B4_4874 at 5394.013 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.060 kb on + strand, within Pf1N1B4_4874 at 5394.125 kb on + strand at 5394.267 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.311 kb on + strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.312 kb on - strand at 5394.376 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.398 kb on + strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.399 kb on - strand at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.489 kb on + strand, within Pf1N1B4_4875 at 5394.490 kb on - strand, within Pf1N1B4_4875 at 5394.504 kb on + strand, within Pf1N1B4_4875 at 5394.505 kb on - strand, within Pf1N1B4_4875 at 5394.537 kb on + strand at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.537 kb on + strand, within Pf1N1B4_4875 at 5394.538 kb on - strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.570 kb on + strand, within Pf1N1B4_4875 at 5394.571 kb on - strand, within Pf1N1B4_4875
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,391,947 + -0.0 5,391,947 + +0.2 5,391,947 + +0.5 5,391,947 + +0.1 5,391,947 + -0.5 5,391,948 - -0.2 5,391,951 + -0.7 5,392,028 + -0.2 5,392,028 + -3.2 5,392,490 + Pf1N1B4_4872 0.61 -0.7 5,392,555 + Pf1N1B4_4872 0.69 +0.3 5,392,556 - Pf1N1B4_4872 0.69 +1.5 5,392,579 + Pf1N1B4_4872 0.72 -0.0 5,392,580 - Pf1N1B4_4872 0.72 +0.5 5,392,782 + -1.1 5,392,782 + +0.6 5,393,002 - Pf1N1B4_4873 0.19 +1.2 5,393,153 - Pf1N1B4_4873 0.39 -1.2 5,393,216 + -2.2 5,393,216 + -0.7 5,393,216 + Pf1N1B4_4873 0.47 +0.3 5,393,217 - Pf1N1B4_4873 0.47 -0.1 5,393,316 + Pf1N1B4_4873 0.60 +0.3 5,393,330 + +1.3 5,393,331 - Pf1N1B4_4873 0.62 +0.5 5,393,331 - Pf1N1B4_4873 0.62 -1.7 5,393,340 - Pf1N1B4_4873 0.64 -0.1 5,393,498 + Pf1N1B4_4873 0.84 +0.8 5,393,550 + -0.7 5,393,550 - +0.1 5,393,550 - -1.7 5,393,550 - +0.1 5,393,550 - -0.7 5,393,616 - -1.4 5,393,679 + -0.3 5,393,694 + -1.2 5,393,694 + -0.1 5,393,694 + +1.8 5,393,694 + -0.8 5,393,694 + +0.3 5,393,695 - -0.2 5,393,695 - -0.3 5,393,695 - +1.5 5,393,695 - -0.8 5,393,695 - +0.6 5,393,695 - +1.3 5,393,794 - +0.4 5,393,900 - +0.7 5,393,927 - Pf1N1B4_4874 0.20 +0.8 5,394,013 + Pf1N1B4_4874 0.54 -2.7 5,394,060 + Pf1N1B4_4874 0.73 +0.9 5,394,060 + Pf1N1B4_4874 0.73 +1.7 5,394,125 + -0.1 5,394,267 + -2.2 5,394,311 + -0.7 5,394,311 + -0.1 5,394,311 + +0.9 5,394,311 + +0.3 5,394,312 - +0.3 5,394,312 - -0.7 5,394,312 - -0.2 5,394,312 - +2.3 5,394,376 + +0.1 5,394,398 + +0.9 5,394,398 + +0.6 5,394,398 + +1.1 5,394,399 - -1.1 5,394,399 - +0.3 5,394,399 - +0.6 5,394,489 + Pf1N1B4_4875 0.23 +0.6 5,394,489 + Pf1N1B4_4875 0.23 -0.7 5,394,490 - Pf1N1B4_4875 0.23 +0.6 5,394,504 + Pf1N1B4_4875 0.27 +0.5 5,394,505 - Pf1N1B4_4875 0.27 -0.7 5,394,537 + -1.7 5,394,537 + Pf1N1B4_4875 0.36 +0.7 5,394,537 + Pf1N1B4_4875 0.36 -0.2 5,394,537 + Pf1N1B4_4875 0.36 +0.5 5,394,538 - Pf1N1B4_4875 0.36 +2.1 5,394,570 + Pf1N1B4_4875 0.44 -1.7 5,394,570 + Pf1N1B4_4875 0.44 -0.7 5,394,571 - Pf1N1B4_4875 0.45 -0.2
Or see this region's nucleotide sequence