Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4319 and Pf1N1B4_4320 overlap by 4 nucleotides Pf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotides Pf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotides
Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104
_4319
Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928
_4320
Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719
_4321
Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567
_4322
Position (kb)
4782
4783 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4781.111 kb on + strand at 4781.112 kb on - strand at 4781.112 kb on - strand at 4781.149 kb on + strand at 4781.149 kb on + strand at 4781.156 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.224 kb on - strand at 4781.224 kb on - strand at 4781.224 kb on - strand at 4781.280 kb on + strand at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.459 kb on - strand, within Pf1N1B4_4319 at 4781.543 kb on - strand, within Pf1N1B4_4319 at 4781.543 kb on - strand, within Pf1N1B4_4319 at 4781.550 kb on + strand, within Pf1N1B4_4319 at 4781.581 kb on + strand, within Pf1N1B4_4319 at 4781.596 kb on - strand, within Pf1N1B4_4319 at 4782.038 kb on - strand at 4782.038 kb on - strand at 4782.156 kb on - strand at 4782.156 kb on - strand at 4782.218 kb on + strand at 4782.218 kb on + strand, within Pf1N1B4_4320 at 4782.218 kb on + strand, within Pf1N1B4_4320 at 4782.219 kb on - strand, within Pf1N1B4_4320 at 4782.219 kb on - strand, within Pf1N1B4_4320 at 4782.301 kb on - strand, within Pf1N1B4_4320 at 4782.423 kb on + strand, within Pf1N1B4_4320 at 4782.447 kb on + strand, within Pf1N1B4_4320 at 4782.447 kb on + strand, within Pf1N1B4_4320 at 4782.452 kb on - strand, within Pf1N1B4_4320 at 4782.452 kb on - strand, within Pf1N1B4_4320 at 4783.150 kb on - strand at 4783.235 kb on + strand, within Pf1N1B4_4321 at 4783.236 kb on - strand, within Pf1N1B4_4321 at 4783.762 kb on + strand at 4783.763 kb on - strand at 4783.862 kb on + strand, within Pf1N1B4_4322
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,781,111 + +0.3 4,781,112 - -0.4 4,781,112 - +0.1 4,781,149 + +0.1 4,781,149 + -1.5 4,781,156 + -1.7 4,781,223 + +0.1 4,781,223 + -1.3 4,781,223 + -1.0 4,781,223 + -2.5 4,781,224 - -1.3 4,781,224 - +0.5 4,781,224 - +0.7 4,781,280 + +1.7 4,781,458 + Pf1N1B4_4319 0.16 -1.3 4,781,458 + Pf1N1B4_4319 0.16 +2.5 4,781,458 + Pf1N1B4_4319 0.16 +0.5 4,781,458 + Pf1N1B4_4319 0.16 -0.3 4,781,459 - Pf1N1B4_4319 0.16 +0.5 4,781,543 - Pf1N1B4_4319 0.27 +0.2 4,781,543 - Pf1N1B4_4319 0.27 +0.7 4,781,550 + Pf1N1B4_4319 0.28 -0.4 4,781,581 + Pf1N1B4_4319 0.32 +0.1 4,781,596 - Pf1N1B4_4319 0.34 -1.0 4,782,038 - +0.8 4,782,038 - -0.3 4,782,156 - -0.5 4,782,156 - -1.1 4,782,218 + -0.5 4,782,218 + Pf1N1B4_4320 0.14 -1.1 4,782,218 + Pf1N1B4_4320 0.14 -0.1 4,782,219 - Pf1N1B4_4320 0.14 -1.5 4,782,219 - Pf1N1B4_4320 0.14 -1.2 4,782,301 - Pf1N1B4_4320 0.24 -0.5 4,782,423 + Pf1N1B4_4320 0.39 +0.7 4,782,447 + Pf1N1B4_4320 0.42 -1.5 4,782,447 + Pf1N1B4_4320 0.42 -0.9 4,782,452 - Pf1N1B4_4320 0.42 -0.9 4,782,452 - Pf1N1B4_4320 0.42 +0.7 4,783,150 - +0.5 4,783,235 + Pf1N1B4_4321 0.39 -1.0 4,783,236 - Pf1N1B4_4321 0.39 -0.5 4,783,762 + -0.5 4,783,763 - -0.9 4,783,862 + Pf1N1B4_4322 0.17 -0.0
Or see this region's nucleotide sequence