Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4320

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4319 and Pf1N1B4_4320 overlap by 4 nucleotidesPf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotidesPf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotides Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104 _4319 Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928 _4320 Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719 _4321 Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567 _4322 Position (kb) 4782 4783Strain fitness (log2 ratio) -2 -1 0 1 2at 4781.111 kb on + strandat 4781.112 kb on - strandat 4781.112 kb on - strandat 4781.149 kb on + strandat 4781.149 kb on + strandat 4781.156 kb on + strandat 4781.223 kb on + strandat 4781.223 kb on + strandat 4781.223 kb on + strandat 4781.223 kb on + strandat 4781.224 kb on - strandat 4781.224 kb on - strandat 4781.224 kb on - strandat 4781.280 kb on + strandat 4781.458 kb on + strand, within Pf1N1B4_4319at 4781.458 kb on + strand, within Pf1N1B4_4319at 4781.458 kb on + strand, within Pf1N1B4_4319at 4781.458 kb on + strand, within Pf1N1B4_4319at 4781.459 kb on - strand, within Pf1N1B4_4319at 4781.543 kb on - strand, within Pf1N1B4_4319at 4781.543 kb on - strand, within Pf1N1B4_4319at 4781.550 kb on + strand, within Pf1N1B4_4319at 4781.581 kb on + strand, within Pf1N1B4_4319at 4781.596 kb on - strand, within Pf1N1B4_4319at 4782.038 kb on - strandat 4782.038 kb on - strandat 4782.156 kb on - strandat 4782.156 kb on - strandat 4782.218 kb on + strandat 4782.218 kb on + strand, within Pf1N1B4_4320at 4782.218 kb on + strand, within Pf1N1B4_4320at 4782.219 kb on - strand, within Pf1N1B4_4320at 4782.219 kb on - strand, within Pf1N1B4_4320at 4782.301 kb on - strand, within Pf1N1B4_4320at 4782.423 kb on + strand, within Pf1N1B4_4320at 4782.447 kb on + strand, within Pf1N1B4_4320at 4782.447 kb on + strand, within Pf1N1B4_4320at 4782.452 kb on - strand, within Pf1N1B4_4320at 4782.452 kb on - strand, within Pf1N1B4_4320at 4783.150 kb on - strandat 4783.235 kb on + strand, within Pf1N1B4_4321at 4783.236 kb on - strand, within Pf1N1B4_4321at 4783.762 kb on + strandat 4783.763 kb on - strandat 4783.862 kb on + strand, within Pf1N1B4_4322

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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4,781,111 + +0.3
4,781,112 - -0.4
4,781,112 - +0.1
4,781,149 + +0.1
4,781,149 + -1.5
4,781,156 + -1.7
4,781,223 + +0.1
4,781,223 + -1.3
4,781,223 + -1.0
4,781,223 + -2.5
4,781,224 - -1.3
4,781,224 - +0.5
4,781,224 - +0.7
4,781,280 + +1.7
4,781,458 + Pf1N1B4_4319 0.16 -1.3
4,781,458 + Pf1N1B4_4319 0.16 +2.5
4,781,458 + Pf1N1B4_4319 0.16 +0.5
4,781,458 + Pf1N1B4_4319 0.16 -0.3
4,781,459 - Pf1N1B4_4319 0.16 +0.5
4,781,543 - Pf1N1B4_4319 0.27 +0.2
4,781,543 - Pf1N1B4_4319 0.27 +0.7
4,781,550 + Pf1N1B4_4319 0.28 -0.4
4,781,581 + Pf1N1B4_4319 0.32 +0.1
4,781,596 - Pf1N1B4_4319 0.34 -1.0
4,782,038 - +0.8
4,782,038 - -0.3
4,782,156 - -0.5
4,782,156 - -1.1
4,782,218 + -0.5
4,782,218 + Pf1N1B4_4320 0.14 -1.1
4,782,218 + Pf1N1B4_4320 0.14 -0.1
4,782,219 - Pf1N1B4_4320 0.14 -1.5
4,782,219 - Pf1N1B4_4320 0.14 -1.2
4,782,301 - Pf1N1B4_4320 0.24 -0.5
4,782,423 + Pf1N1B4_4320 0.39 +0.7
4,782,447 + Pf1N1B4_4320 0.42 -1.5
4,782,447 + Pf1N1B4_4320 0.42 -0.9
4,782,452 - Pf1N1B4_4320 0.42 -0.9
4,782,452 - Pf1N1B4_4320 0.42 +0.7
4,783,150 - +0.5
4,783,235 + Pf1N1B4_4321 0.39 -1.0
4,783,236 - Pf1N1B4_4321 0.39 -0.5
4,783,762 + -0.5
4,783,763 - -0.9
4,783,862 + Pf1N1B4_4322 0.17 -0.0

Or see this region's nucleotide sequence