Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_371 and Pf1N1B4_372 are separated by 105 nucleotides Pf1N1B4_372 and Pf1N1B4_373 are separated by 323 nucleotides
Pf1N1B4_371 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 438,340 to 439,572
_371
Pf1N1B4_372 - UDP-galactose-lipid carrier transferase (EC 2.-.-.-), at 439,678 to 441,201
_372
Pf1N1B4_373 - 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC, at 441,525 to 443,507
_373
Position (kb) 439
440
441
442 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 438.983 kb on + strand at 438.983 kb on + strand, within Pf1N1B4_371 at 438.984 kb on - strand at 439.068 kb on - strand, within Pf1N1B4_371 at 439.068 kb on - strand, within Pf1N1B4_371 at 439.068 kb on - strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand, within Pf1N1B4_371 at 439.139 kb on + strand at 439.139 kb on + strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.140 kb on - strand, within Pf1N1B4_371 at 439.577 kb on + strand at 439.577 kb on + strand at 439.577 kb on + strand at 439.577 kb on + strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.578 kb on - strand at 439.618 kb on + strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.619 kb on - strand at 439.629 kb on + strand at 439.630 kb on - strand at 439.816 kb on + strand at 439.817 kb on - strand at 440.171 kb on + strand, within Pf1N1B4_372 at 440.223 kb on - strand, within Pf1N1B4_372 at 440.381 kb on + strand, within Pf1N1B4_372 at 440.382 kb on - strand, within Pf1N1B4_372 at 440.547 kb on + strand, within Pf1N1B4_372 at 440.549 kb on + strand, within Pf1N1B4_372 at 440.550 kb on - strand, within Pf1N1B4_372 at 440.624 kb on + strand, within Pf1N1B4_372 at 440.624 kb on + strand at 440.625 kb on - strand, within Pf1N1B4_372 at 440.643 kb on - strand, within Pf1N1B4_372 at 441.155 kb on + strand at 441.155 kb on + strand at 441.155 kb on + strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.156 kb on - strand at 441.201 kb on + strand at 441.201 kb on + strand at 441.202 kb on - strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.250 kb on + strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.251 kb on - strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.254 kb on + strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.255 kb on - strand at 441.360 kb on + strand at 441.360 kb on + strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.361 kb on - strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.364 kb on + strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.365 kb on - strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.603 kb on + strand at 441.900 kb on + strand, within Pf1N1B4_373
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 438,983 + -1.1 438,983 + Pf1N1B4_371 0.52 +0.3 438,984 - -1.7 439,068 - Pf1N1B4_371 0.59 -0.3 439,068 - Pf1N1B4_371 0.59 -1.7 439,068 - Pf1N1B4_371 0.59 -1.1 439,139 + Pf1N1B4_371 0.65 -0.1 439,139 + Pf1N1B4_371 0.65 -1.4 439,139 + Pf1N1B4_371 0.65 -0.5 439,139 + Pf1N1B4_371 0.65 -1.5 439,139 + Pf1N1B4_371 0.65 -3.3 439,139 + Pf1N1B4_371 0.65 +0.9 439,139 + +0.9 439,139 + Pf1N1B4_371 0.65 +1.3 439,140 - Pf1N1B4_371 0.65 -0.3 439,140 - Pf1N1B4_371 0.65 -0.5 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 +1.5 439,140 - Pf1N1B4_371 0.65 -0.1 439,140 - Pf1N1B4_371 0.65 -0.6 439,577 + +1.9 439,577 + -0.7 439,577 + +0.2 439,577 + -0.3 439,578 - +0.2 439,578 - -0.1 439,578 - +2.5 439,578 - -0.7 439,618 + -0.5 439,619 - -0.1 439,619 - -1.1 439,619 - +2.0 439,619 - +1.9 439,629 + +0.2 439,630 - -2.1 439,816 + -0.1 439,817 - -0.5 440,171 + Pf1N1B4_372 0.32 -2.1 440,223 - Pf1N1B4_372 0.36 -1.1 440,381 + Pf1N1B4_372 0.46 +0.9 440,382 - Pf1N1B4_372 0.46 -0.4 440,547 + Pf1N1B4_372 0.57 -0.5 440,549 + Pf1N1B4_372 0.57 -0.1 440,550 - Pf1N1B4_372 0.57 -0.1 440,624 + Pf1N1B4_372 0.62 -1.5 440,624 + -0.5 440,625 - Pf1N1B4_372 0.62 -1.1 440,643 - Pf1N1B4_372 0.63 -0.7 441,155 + +2.3 441,155 + -0.4 441,155 + -0.5 441,156 - -1.7 441,156 - +0.8 441,156 - -0.9 441,201 + +0.5 441,201 + +0.2 441,202 - +2.2 441,250 + +0.5 441,250 + +0.4 441,250 + +0.9 441,250 + -0.1 441,250 + +1.5 441,250 + -1.1 441,250 + +1.5 441,250 + +0.6 441,250 + +0.1 441,251 - +0.5 441,251 - -1.5 441,251 - -1.5 441,251 - +1.0 441,251 - +0.4 441,254 + -1.1 441,254 + +1.5 441,254 + -0.1 441,255 - -1.1 441,255 - -0.1 441,255 - +0.3 441,255 - +1.0 441,360 + -0.5 441,360 + +0.7 441,361 - +0.9 441,361 - -1.1 441,361 - +1.7 441,361 - -1.1 441,364 + +0.5 441,364 + -1.3 441,364 + -0.1 441,364 + +0.6 441,364 + +0.9 441,364 + +0.6 441,365 - +1.7 441,365 - -0.9 441,365 - -0.5 441,365 - -0.1 441,365 - -0.7 441,365 - -0.7 441,603 + +0.9 441,603 + +0.9 441,603 + -0.9 441,603 + -1.1 441,603 + -0.1 441,900 + Pf1N1B4_373 0.19 -0.1
Or see this region's nucleotide sequence