Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3526 and Pf1N1B4_3527 are separated by 8 nucleotides Pf1N1B4_3527 and Pf1N1B4_3528 are separated by 68 nucleotides Pf1N1B4_3528 and Pf1N1B4_3529 are separated by 26 nucleotides
Pf1N1B4_3526 - Glutathione S-transferase (EC 2.5.1.18), at 3,931,944 to 3,932,603
_3526
Pf1N1B4_3527 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 3,932,612 to 3,933,418
_3527
Pf1N1B4_3528 - Putative membrane protein, at 3,933,487 to 3,934,542
_3528
Pf1N1B4_3529 - Membrane bound c-di-GMP receptor LapD, at 3,934,569 to 3,936,509
_3529
Position (kb)
3933
3934
3935 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3932.608 kb on - strand at 3932.672 kb on - strand at 3932.849 kb on + strand at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.850 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3932.932 kb on - strand, within Pf1N1B4_3527 at 3933.005 kb on + strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.030 kb on - strand, within Pf1N1B4_3527 at 3933.034 kb on - strand, within Pf1N1B4_3527 at 3933.080 kb on + strand, within Pf1N1B4_3527 at 3933.081 kb on - strand, within Pf1N1B4_3527 at 3933.110 kb on + strand, within Pf1N1B4_3527 at 3933.351 kb on + strand at 3933.454 kb on - strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.484 kb on + strand at 3933.485 kb on - strand at 3933.485 kb on - strand at 3933.773 kb on + strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.774 kb on - strand, within Pf1N1B4_3528 at 3933.978 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.075 kb on + strand, within Pf1N1B4_3528 at 3934.076 kb on - strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.352 kb on + strand, within Pf1N1B4_3528 at 3934.353 kb on - strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.356 kb on + strand, within Pf1N1B4_3528 at 3934.357 kb on - strand, within Pf1N1B4_3528 at 3934.483 kb on + strand at 3934.632 kb on + strand at 3934.755 kb on - strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.756 kb on + strand at 3934.757 kb on - strand at 3934.757 kb on - strand at 3935.004 kb on - strand, within Pf1N1B4_3529 at 3935.227 kb on + strand, within Pf1N1B4_3529 at 3935.227 kb on + strand, within Pf1N1B4_3529 at 3935.227 kb on + strand, within Pf1N1B4_3529 at 3935.227 kb on + strand, within Pf1N1B4_3529 at 3935.228 kb on - strand, within Pf1N1B4_3529 at 3935.228 kb on - strand, within Pf1N1B4_3529 at 3935.228 kb on - strand, within Pf1N1B4_3529 at 3935.320 kb on + strand, within Pf1N1B4_3529 at 3935.323 kb on - strand, within Pf1N1B4_3529 at 3935.459 kb on + strand, within Pf1N1B4_3529
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,932,608 - -0.1 3,932,672 - -0.1 3,932,849 + -0.1 3,932,850 - Pf1N1B4_3527 0.29 +0.0 3,932,850 - Pf1N1B4_3527 0.29 +1.1 3,932,850 - Pf1N1B4_3527 0.29 +0.4 3,932,932 - Pf1N1B4_3527 0.40 -0.1 3,932,932 - Pf1N1B4_3527 0.40 -0.1 3,933,005 + Pf1N1B4_3527 0.49 -0.3 3,933,030 - Pf1N1B4_3527 0.52 +0.9 3,933,030 - Pf1N1B4_3527 0.52 +0.4 3,933,034 - Pf1N1B4_3527 0.52 -0.1 3,933,080 + Pf1N1B4_3527 0.58 +1.4 3,933,081 - Pf1N1B4_3527 0.58 -1.1 3,933,110 + Pf1N1B4_3527 0.62 +1.0 3,933,351 + -0.1 3,933,454 - +1.7 3,933,484 + -0.7 3,933,484 + +0.9 3,933,484 + -0.7 3,933,484 + -1.1 3,933,484 + -0.6 3,933,485 - -2.5 3,933,485 - +0.6 3,933,773 + Pf1N1B4_3528 0.27 +1.4 3,933,774 - Pf1N1B4_3528 0.27 +0.3 3,933,774 - Pf1N1B4_3528 0.27 -0.1 3,933,774 - Pf1N1B4_3528 0.27 -0.5 3,933,978 + Pf1N1B4_3528 0.46 +0.9 3,934,075 + Pf1N1B4_3528 0.56 +0.9 3,934,075 + Pf1N1B4_3528 0.56 -1.5 3,934,075 + Pf1N1B4_3528 0.56 -2.1 3,934,075 + Pf1N1B4_3528 0.56 +0.4 3,934,075 + Pf1N1B4_3528 0.56 +0.4 3,934,076 - -0.1 3,934,352 + Pf1N1B4_3528 0.82 -2.7 3,934,352 + Pf1N1B4_3528 0.82 -0.1 3,934,352 + +0.9 3,934,352 + Pf1N1B4_3528 0.82 +0.6 3,934,352 + Pf1N1B4_3528 0.82 -0.6 3,934,352 + Pf1N1B4_3528 0.82 -0.4 3,934,352 + Pf1N1B4_3528 0.82 -1.1 3,934,353 - +0.6 3,934,356 + Pf1N1B4_3528 0.82 +0.6 3,934,356 + +1.9 3,934,356 + Pf1N1B4_3528 0.82 -0.5 3,934,356 + Pf1N1B4_3528 0.82 -0.9 3,934,357 - Pf1N1B4_3528 0.82 +2.0 3,934,483 + +0.1 3,934,632 + -0.2 3,934,755 - -0.7 3,934,756 + +0.7 3,934,756 + +0.0 3,934,756 + -0.1 3,934,756 + -1.5 3,934,756 + -0.7 3,934,756 + -0.1 3,934,756 + +1.0 3,934,756 + -2.9 3,934,756 + -1.0 3,934,756 + -1.1 3,934,757 - -0.1 3,934,757 - +0.9 3,935,004 - Pf1N1B4_3529 0.22 -1.7 3,935,227 + Pf1N1B4_3529 0.34 -0.1 3,935,227 + Pf1N1B4_3529 0.34 +0.4 3,935,227 + Pf1N1B4_3529 0.34 -0.6 3,935,227 + Pf1N1B4_3529 0.34 -0.1 3,935,228 - Pf1N1B4_3529 0.34 +0.5 3,935,228 - Pf1N1B4_3529 0.34 -0.9 3,935,228 - Pf1N1B4_3529 0.34 +0.9 3,935,320 + Pf1N1B4_3529 0.39 -0.1 3,935,323 - Pf1N1B4_3529 0.39 -0.1 3,935,459 + Pf1N1B4_3529 0.46 +0.3
Or see this region's nucleotide sequence