Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3385 and Pf1N1B4_3386 are separated by 133 nucleotides Pf1N1B4_3386 and Pf1N1B4_3387 are separated by 129 nucleotides
Pf1N1B4_3385 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26), at 3,773,946 to 3,775,526
_3385
Pf1N1B4_3386 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,775,660 to 3,776,568
_3386
Pf1N1B4_3387 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 3,776,698 to 3,778,419
_3387
Position (kb)
3775
3776
3777 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3774.695 kb on + strand, within Pf1N1B4_3385 at 3774.695 kb on + strand, within Pf1N1B4_3385 at 3774.848 kb on + strand, within Pf1N1B4_3385 at 3774.972 kb on + strand, within Pf1N1B4_3385 at 3775.242 kb on + strand, within Pf1N1B4_3385 at 3775.432 kb on - strand at 3775.516 kb on + strand at 3775.516 kb on + strand at 3775.516 kb on + strand at 3775.517 kb on - strand at 3775.661 kb on + strand at 3775.709 kb on - strand at 3775.866 kb on - strand, within Pf1N1B4_3386 at 3775.866 kb on - strand, within Pf1N1B4_3386 at 3775.866 kb on - strand, within Pf1N1B4_3386 at 3775.869 kb on + strand, within Pf1N1B4_3386 at 3775.869 kb on + strand, within Pf1N1B4_3386 at 3775.870 kb on - strand, within Pf1N1B4_3386 at 3775.870 kb on - strand, within Pf1N1B4_3386 at 3775.870 kb on - strand, within Pf1N1B4_3386 at 3775.874 kb on + strand at 3775.875 kb on - strand, within Pf1N1B4_3386 at 3775.875 kb on - strand, within Pf1N1B4_3386 at 3775.875 kb on - strand, within Pf1N1B4_3386 at 3776.324 kb on + strand at 3776.324 kb on + strand, within Pf1N1B4_3386 at 3776.544 kb on + strand at 3776.544 kb on + strand at 3776.544 kb on + strand at 3776.544 kb on + strand at 3776.545 kb on - strand at 3776.697 kb on + strand at 3776.697 kb on + strand at 3776.697 kb on + strand at 3776.959 kb on + strand at 3776.959 kb on + strand, within Pf1N1B4_3387 at 3776.959 kb on + strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3776.960 kb on - strand, within Pf1N1B4_3387 at 3777.124 kb on + strand, within Pf1N1B4_3387 at 3777.124 kb on + strand, within Pf1N1B4_3387 at 3777.124 kb on + strand, within Pf1N1B4_3387 at 3777.124 kb on + strand, within Pf1N1B4_3387 at 3777.124 kb on + strand, within Pf1N1B4_3387 at 3777.125 kb on - strand, within Pf1N1B4_3387 at 3777.125 kb on - strand, within Pf1N1B4_3387 at 3777.125 kb on - strand, within Pf1N1B4_3387 at 3777.125 kb on - strand, within Pf1N1B4_3387 at 3777.125 kb on - strand at 3777.193 kb on + strand, within Pf1N1B4_3387 at 3777.307 kb on + strand, within Pf1N1B4_3387 at 3777.343 kb on + strand, within Pf1N1B4_3387 at 3777.343 kb on + strand, within Pf1N1B4_3387 at 3777.344 kb on - strand, within Pf1N1B4_3387 at 3777.344 kb on - strand, within Pf1N1B4_3387 at 3777.344 kb on - strand, within Pf1N1B4_3387 at 3777.469 kb on + strand, within Pf1N1B4_3387 at 3777.469 kb on + strand, within Pf1N1B4_3387 at 3777.470 kb on - strand, within Pf1N1B4_3387 at 3777.470 kb on - strand, within Pf1N1B4_3387 at 3777.470 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387 at 3777.554 kb on - strand, within Pf1N1B4_3387
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,774,695 + Pf1N1B4_3385 0.47 -0.3 3,774,695 + Pf1N1B4_3385 0.47 +0.9 3,774,848 + Pf1N1B4_3385 0.57 -1.1 3,774,972 + Pf1N1B4_3385 0.65 -1.7 3,775,242 + Pf1N1B4_3385 0.82 +0.3 3,775,432 - -0.5 3,775,516 + +1.5 3,775,516 + -1.0 3,775,516 + -0.3 3,775,517 - -0.7 3,775,661 + +0.2 3,775,709 - -1.9 3,775,866 - Pf1N1B4_3386 0.23 -1.7 3,775,866 - Pf1N1B4_3386 0.23 -0.8 3,775,866 - Pf1N1B4_3386 0.23 -1.4 3,775,869 + Pf1N1B4_3386 0.23 -0.8 3,775,869 + Pf1N1B4_3386 0.23 +2.2 3,775,870 - Pf1N1B4_3386 0.23 -2.1 3,775,870 - Pf1N1B4_3386 0.23 +0.2 3,775,870 - Pf1N1B4_3386 0.23 +0.7 3,775,874 + +0.9 3,775,875 - Pf1N1B4_3386 0.24 -0.5 3,775,875 - Pf1N1B4_3386 0.24 -0.9 3,775,875 - Pf1N1B4_3386 0.24 -1.1 3,776,324 + +1.2 3,776,324 + Pf1N1B4_3386 0.73 -0.2 3,776,544 + +1.5 3,776,544 + +0.5 3,776,544 + -0.5 3,776,544 + -1.2 3,776,545 - -0.1 3,776,697 + -1.1 3,776,697 + +0.2 3,776,697 + -0.8 3,776,959 + -0.1 3,776,959 + Pf1N1B4_3387 0.15 -0.1 3,776,959 + Pf1N1B4_3387 0.15 -1.0 3,776,960 - Pf1N1B4_3387 0.15 +0.2 3,776,960 - Pf1N1B4_3387 0.15 +0.9 3,776,960 - Pf1N1B4_3387 0.15 +0.5 3,776,960 - Pf1N1B4_3387 0.15 +0.9 3,776,960 - Pf1N1B4_3387 0.15 -0.1 3,776,960 - Pf1N1B4_3387 0.15 -1.1 3,777,124 + Pf1N1B4_3387 0.25 -1.5 3,777,124 + Pf1N1B4_3387 0.25 +0.2 3,777,124 + Pf1N1B4_3387 0.25 +0.9 3,777,124 + Pf1N1B4_3387 0.25 +0.3 3,777,124 + Pf1N1B4_3387 0.25 -0.1 3,777,125 - Pf1N1B4_3387 0.25 +0.3 3,777,125 - Pf1N1B4_3387 0.25 +0.5 3,777,125 - Pf1N1B4_3387 0.25 -1.7 3,777,125 - Pf1N1B4_3387 0.25 -1.7 3,777,125 - +1.5 3,777,193 + Pf1N1B4_3387 0.29 +0.5 3,777,307 + Pf1N1B4_3387 0.35 +1.5 3,777,343 + Pf1N1B4_3387 0.37 -1.7 3,777,343 + Pf1N1B4_3387 0.37 +1.9 3,777,344 - Pf1N1B4_3387 0.38 +0.9 3,777,344 - Pf1N1B4_3387 0.38 +0.3 3,777,344 - Pf1N1B4_3387 0.38 +0.5 3,777,469 + Pf1N1B4_3387 0.45 -0.3 3,777,469 + Pf1N1B4_3387 0.45 +0.4 3,777,470 - Pf1N1B4_3387 0.45 -1.4 3,777,470 - Pf1N1B4_3387 0.45 +0.1 3,777,470 - Pf1N1B4_3387 0.45 -0.1 3,777,554 - Pf1N1B4_3387 0.50 -0.5 3,777,554 - Pf1N1B4_3387 0.50 +0.5 3,777,554 - Pf1N1B4_3387 0.50 -0.1 3,777,554 - Pf1N1B4_3387 0.50 +0.6 3,777,554 - Pf1N1B4_3387 0.50 -1.6 3,777,554 - Pf1N1B4_3387 0.50 +0.1 3,777,554 - Pf1N1B4_3387 0.50 -1.5
Or see this region's nucleotide sequence