Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3364 and Pf1N1B4_3365 are separated by 147 nucleotides Pf1N1B4_3365 and Pf1N1B4_3366 are separated by 78 nucleotides Pf1N1B4_3366 and Pf1N1B4_3367 overlap by 4 nucleotides
Pf1N1B4_3364 - Cyn operon transcriptional activator, at 3,751,948 to 3,752,865
_3364
Pf1N1B4_3365 - Sialic acid transporter (permease) NanT, at 3,753,013 to 3,754,296
_3365
Pf1N1B4_3366 - Lactam utilization protein LamB, at 3,754,375 to 3,755,127
_3366
Pf1N1B4_3367 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,755,124 to 3,755,828
_3367
Position (kb)
3753
3754
3755 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3752.182 kb on + strand, within Pf1N1B4_3364 at 3752.183 kb on - strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.345 kb on + strand, within Pf1N1B4_3364 at 3752.882 kb on + strand at 3752.882 kb on + strand at 3752.904 kb on + strand at 3752.904 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3753.074 kb on - strand at 3753.102 kb on + strand at 3753.102 kb on + strand at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.833 kb on + strand, within Pf1N1B4_3365 at 3753.834 kb on - strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.977 kb on - strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.316 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.360 kb on - strand at 3754.389 kb on - strand at 3754.571 kb on - strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand at 3754.721 kb on - strand, within Pf1N1B4_3366 at 3754.852 kb on + strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.895 kb on - strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand at 3755.052 kb on - strand at 3755.137 kb on + strand at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.274 kb on + strand, within Pf1N1B4_3367 at 3755.295 kb on + strand, within Pf1N1B4_3367
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,752,182 + Pf1N1B4_3364 0.25 -1.1 3,752,183 - Pf1N1B4_3364 0.26 +1.4 3,752,295 + Pf1N1B4_3364 0.38 -0.4 3,752,295 + Pf1N1B4_3364 0.38 -0.1 3,752,334 - Pf1N1B4_3364 0.42 +0.8 3,752,334 - Pf1N1B4_3364 0.42 -1.7 3,752,345 + Pf1N1B4_3364 0.43 +0.5 3,752,882 + -0.9 3,752,882 + +0.2 3,752,904 + +1.2 3,752,904 + +1.9 3,752,984 + -0.1 3,752,984 + -0.9 3,752,984 + -0.5 3,753,074 - -2.4 3,753,102 + +0.5 3,753,102 + +0.2 3,753,364 + Pf1N1B4_3365 0.27 -2.1 3,753,364 + Pf1N1B4_3365 0.27 -1.1 3,753,364 + +1.5 3,753,365 - Pf1N1B4_3365 0.27 -0.8 3,753,365 - Pf1N1B4_3365 0.27 -0.1 3,753,669 + Pf1N1B4_3365 0.51 +0.1 3,753,669 + Pf1N1B4_3365 0.51 -0.5 3,753,778 + Pf1N1B4_3365 0.60 -1.1 3,753,778 + Pf1N1B4_3365 0.60 +1.2 3,753,833 + Pf1N1B4_3365 0.64 -1.1 3,753,834 - Pf1N1B4_3365 0.64 -1.7 3,753,877 + Pf1N1B4_3365 0.67 +1.1 3,753,877 + Pf1N1B4_3365 0.67 -0.8 3,753,877 + Pf1N1B4_3365 0.67 -1.1 3,753,977 - Pf1N1B4_3365 0.75 -0.1 3,754,123 + Pf1N1B4_3365 0.86 -0.1 3,754,123 + Pf1N1B4_3365 0.86 -2.1 3,754,123 + Pf1N1B4_3365 0.86 -0.5 3,754,316 + -0.3 3,754,330 + -2.1 3,754,330 + -2.1 3,754,330 + -0.7 3,754,330 + -2.2 3,754,330 + +1.2 3,754,331 - -0.1 3,754,331 - +1.9 3,754,331 - -0.1 3,754,360 - +1.9 3,754,389 - +0.1 3,754,571 - Pf1N1B4_3366 0.26 +1.5 3,754,609 + Pf1N1B4_3366 0.31 -1.1 3,754,609 + Pf1N1B4_3366 0.31 +0.2 3,754,696 + Pf1N1B4_3366 0.43 -1.1 3,754,696 + Pf1N1B4_3366 0.43 +0.8 3,754,696 + Pf1N1B4_3366 0.43 -1.1 3,754,697 - Pf1N1B4_3366 0.43 -2.4 3,754,697 - Pf1N1B4_3366 0.43 +0.3 3,754,720 + Pf1N1B4_3366 0.46 -0.5 3,754,720 + Pf1N1B4_3366 0.46 -1.1 3,754,720 + Pf1N1B4_3366 0.46 -0.4 3,754,720 + Pf1N1B4_3366 0.46 +0.6 3,754,720 + Pf1N1B4_3366 0.46 -1.1 3,754,720 + -0.1 3,754,721 - Pf1N1B4_3366 0.46 -2.4 3,754,852 + Pf1N1B4_3366 0.63 +0.7 3,754,853 - Pf1N1B4_3366 0.63 -0.1 3,754,853 - Pf1N1B4_3366 0.63 -0.1 3,754,894 + Pf1N1B4_3366 0.69 -0.7 3,754,894 + Pf1N1B4_3366 0.69 -0.1 3,754,895 - Pf1N1B4_3366 0.69 -1.7 3,755,040 + Pf1N1B4_3366 0.88 -0.9 3,755,040 + Pf1N1B4_3366 0.88 -0.1 3,755,040 + Pf1N1B4_3366 0.88 -1.1 3,755,041 - Pf1N1B4_3366 0.88 -0.5 3,755,041 - Pf1N1B4_3366 0.88 -0.7 3,755,051 + Pf1N1B4_3366 0.90 +0.7 3,755,051 + Pf1N1B4_3366 0.90 +1.7 3,755,051 + +1.5 3,755,052 - -1.5 3,755,137 + +0.3 3,755,198 + Pf1N1B4_3367 0.10 +0.3 3,755,198 + Pf1N1B4_3367 0.10 -0.3 3,755,274 + Pf1N1B4_3367 0.21 -1.5 3,755,295 + Pf1N1B4_3367 0.24 +0.1
Or see this region's nucleotide sequence