Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_242 and Pf1N1B4_243 are separated by 1 nucleotides Pf1N1B4_243 and Pf1N1B4_244 overlap by 105 nucleotides Pf1N1B4_244 and Pf1N1B4_245 are separated by 219 nucleotides Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides
Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649
_242
Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925
_243
Pf1N1B4_244 - hypothetical protein, at 302,821 to 302,946
_244
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Position (kb) 301
302
303 Strain fitness (log2 ratio) -2
-1
0
1
2 at 300.677 kb on - strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.208 kb on - strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.219 kb on - strand, within Pf1N1B4_242 at 301.342 kb on + strand, within Pf1N1B4_242 at 301.384 kb on + strand, within Pf1N1B4_242 at 301.621 kb on - strand at 301.703 kb on + strand at 301.704 kb on - strand at 301.704 kb on - strand at 301.704 kb on - strand at 302.188 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.588 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand at 302.656 kb on + strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.667 kb on - strand, within Pf1N1B4_243 at 302.820 kb on + strand at 302.820 kb on + strand at 302.820 kb on + strand at 302.821 kb on - strand at 303.008 kb on + strand at 303.009 kb on - strand at 303.079 kb on + strand at 303.080 kb on - strand at 303.104 kb on + strand at 303.105 kb on - strand at 303.128 kb on + strand at 303.128 kb on + strand at 303.129 kb on - strand at 303.160 kb on + strand at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 300,677 - Pf1N1B4_242 0.43 -0.2 300,677 - Pf1N1B4_242 0.43 +0.2 301,207 + Pf1N1B4_242 0.74 +0.5 301,207 + Pf1N1B4_242 0.74 -1.3 301,207 + Pf1N1B4_242 0.74 -0.2 301,208 - Pf1N1B4_242 0.74 +0.2 301,218 + Pf1N1B4_242 0.75 -0.8 301,218 + Pf1N1B4_242 0.75 +0.4 301,218 + Pf1N1B4_242 0.75 +1.0 301,219 - Pf1N1B4_242 0.75 -1.5 301,342 + Pf1N1B4_242 0.82 -1.5 301,384 + Pf1N1B4_242 0.84 -1.2 301,621 - -0.8 301,703 + -1.0 301,704 - -0.2 301,704 - -0.6 301,704 - +1.8 302,188 + Pf1N1B4_243 0.42 -0.8 302,456 + Pf1N1B4_243 0.63 +2.1 302,456 + Pf1N1B4_243 0.63 -0.6 302,456 + Pf1N1B4_243 0.63 -1.8 302,457 - Pf1N1B4_243 0.63 -1.2 302,457 - Pf1N1B4_243 0.63 -0.6 302,588 + Pf1N1B4_243 0.73 -0.7 302,656 + Pf1N1B4_243 0.79 -0.0 302,656 + Pf1N1B4_243 0.79 -1.5 302,656 + +0.8 302,656 + Pf1N1B4_243 0.79 -1.2 302,657 - Pf1N1B4_243 0.79 +0.8 302,657 - Pf1N1B4_243 0.79 -1.8 302,657 - -0.2 302,658 + Pf1N1B4_243 0.79 -0.5 302,658 + Pf1N1B4_243 0.79 -0.2 302,658 + Pf1N1B4_243 0.79 -0.6 302,659 - Pf1N1B4_243 0.79 -1.2 302,659 - Pf1N1B4_243 0.79 -0.9 302,666 + Pf1N1B4_243 0.80 -0.9 302,666 + Pf1N1B4_243 0.80 -0.9 302,667 - Pf1N1B4_243 0.80 +1.8 302,820 + +2.1 302,820 + +0.4 302,820 + -1.2 302,821 - +0.5 303,008 + -0.2 303,009 - +0.5 303,079 + -1.8 303,080 - +0.1 303,104 + -1.2 303,105 - -0.0 303,128 + -0.2 303,128 + -0.8 303,129 - +0.3 303,160 + -0.2 303,397 + Pf1N1B4_245 0.73 +0.8 303,397 + Pf1N1B4_245 0.73 +2.1 303,482 - -0.6 303,482 - +0.1 303,716 + Pf1N1B4_246 0.60 +0.8 303,752 + Pf1N1B4_246 0.69 -0.2 303,752 + Pf1N1B4_246 0.69 -0.8 303,752 + Pf1N1B4_246 0.69 -0.8 303,752 + Pf1N1B4_246 0.69 -0.2 303,752 + Pf1N1B4_246 0.69 +0.8 303,752 + Pf1N1B4_246 0.69 +1.1 303,752 + Pf1N1B4_246 0.69 -0.5 303,752 + Pf1N1B4_246 0.69 -0.2 303,752 + Pf1N1B4_246 0.69 +0.8 303,752 + Pf1N1B4_246 0.69 +0.4 303,753 - Pf1N1B4_246 0.69 -0.5
Or see this region's nucleotide sequence