Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2420 and Pf1N1B4_2421 overlap by 4 nucleotides Pf1N1B4_2421 and Pf1N1B4_2422 are separated by 77 nucleotides
Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300
_2420
Pf1N1B4_2421 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 2,746,297 to 2,747,142
_2421
Pf1N1B4_2422 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 2,747,220 to 2,748,170
_2422
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.513 kb on + strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.555 kb on + strand, within Pf1N1B4_2420 at 2746.131 kb on - strand, within Pf1N1B4_2420 at 2746.312 kb on + strand at 2746.312 kb on + strand at 2746.313 kb on - strand at 2746.339 kb on - strand at 2746.364 kb on - strand at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.626 kb on - strand, within Pf1N1B4_2421 at 2746.710 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.775 kb on + strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.776 kb on - strand, within Pf1N1B4_2421 at 2746.969 kb on + strand, within Pf1N1B4_2421 at 2747.108 kb on + strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.109 kb on - strand at 2747.122 kb on + strand at 2747.123 kb on - strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.170 kb on + strand at 2747.265 kb on - strand at 2747.473 kb on + strand at 2747.473 kb on + strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.474 kb on - strand, within Pf1N1B4_2422 at 2747.506 kb on + strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.507 kb on - strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.527 kb on + strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.528 kb on - strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.737 kb on + strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.738 kb on - strand, within Pf1N1B4_2422 at 2747.802 kb on - strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.803 kb on + strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.804 kb on - strand, within Pf1N1B4_2422 at 2747.830 kb on + strand, within Pf1N1B4_2422 at 2747.831 kb on - strand, within Pf1N1B4_2422 at 2747.849 kb on + strand, within Pf1N1B4_2422 at 2747.922 kb on - strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.965 kb on + strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2747.966 kb on - strand, within Pf1N1B4_2422 at 2748.004 kb on + strand, within Pf1N1B4_2422 at 2748.005 kb on - strand, within Pf1N1B4_2422
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,745,473 + Pf1N1B4_2420 0.30 +0.3 2,745,473 + Pf1N1B4_2420 0.30 -0.4 2,745,474 - Pf1N1B4_2420 0.30 -0.5 2,745,474 - Pf1N1B4_2420 0.30 +0.8 2,745,474 - Pf1N1B4_2420 0.30 -0.3 2,745,474 - Pf1N1B4_2420 0.30 +0.9 2,745,474 - Pf1N1B4_2420 0.30 -1.3 2,745,474 - Pf1N1B4_2420 0.30 -0.6 2,745,474 - Pf1N1B4_2420 0.30 -0.7 2,745,474 - Pf1N1B4_2420 0.30 -0.7 2,745,513 + Pf1N1B4_2420 0.33 +0.3 2,745,514 - Pf1N1B4_2420 0.33 +1.0 2,745,514 - Pf1N1B4_2420 0.33 +0.3 2,745,555 + Pf1N1B4_2420 0.37 +0.3 2,746,131 - Pf1N1B4_2420 0.86 -1.0 2,746,312 + -1.3 2,746,312 + +1.9 2,746,313 - +0.6 2,746,339 - -1.1 2,746,364 - -0.4 2,746,626 - Pf1N1B4_2421 0.39 -0.4 2,746,626 - Pf1N1B4_2421 0.39 -0.9 2,746,710 + Pf1N1B4_2421 0.49 -1.5 2,746,775 + Pf1N1B4_2421 0.57 -1.4 2,746,775 + Pf1N1B4_2421 0.57 +0.3 2,746,776 - Pf1N1B4_2421 0.57 +0.3 2,746,776 - +0.3 2,746,776 - Pf1N1B4_2421 0.57 -1.3 2,746,776 - Pf1N1B4_2421 0.57 -0.7 2,746,969 + Pf1N1B4_2421 0.79 +0.3 2,747,108 + -0.7 2,747,109 - +0.3 2,747,109 - -0.2 2,747,109 - -0.0 2,747,122 + -0.2 2,747,123 - -0.3 2,747,170 + +0.9 2,747,170 + -0.2 2,747,170 + +0.3 2,747,265 - +1.1 2,747,473 + -0.3 2,747,473 + Pf1N1B4_2422 0.27 -0.4 2,747,474 - Pf1N1B4_2422 0.27 +1.4 2,747,474 - Pf1N1B4_2422 0.27 -0.1 2,747,474 - Pf1N1B4_2422 0.27 -0.7 2,747,474 - Pf1N1B4_2422 0.27 -0.0 2,747,506 + Pf1N1B4_2422 0.30 +2.6 2,747,507 - Pf1N1B4_2422 0.30 +0.3 2,747,507 - Pf1N1B4_2422 0.30 +1.1 2,747,527 + Pf1N1B4_2422 0.32 -2.0 2,747,527 + Pf1N1B4_2422 0.32 -0.7 2,747,527 + Pf1N1B4_2422 0.32 +1.3 2,747,528 - Pf1N1B4_2422 0.32 +1.6 2,747,528 - Pf1N1B4_2422 0.32 -1.7 2,747,528 - Pf1N1B4_2422 0.32 -1.5 2,747,528 - Pf1N1B4_2422 0.32 +0.2 2,747,528 - Pf1N1B4_2422 0.32 -0.3 2,747,737 + Pf1N1B4_2422 0.54 +0.7 2,747,737 + Pf1N1B4_2422 0.54 +2.3 2,747,738 - Pf1N1B4_2422 0.54 +0.3 2,747,738 - Pf1N1B4_2422 0.54 -0.6 2,747,738 - Pf1N1B4_2422 0.54 +1.9 2,747,802 - Pf1N1B4_2422 0.61 +0.9 2,747,803 + Pf1N1B4_2422 0.61 +0.9 2,747,803 + Pf1N1B4_2422 0.61 -0.3 2,747,803 + Pf1N1B4_2422 0.61 +1.7 2,747,803 + Pf1N1B4_2422 0.61 -0.1 2,747,803 + Pf1N1B4_2422 0.61 +0.6 2,747,803 + Pf1N1B4_2422 0.61 -0.7 2,747,803 + Pf1N1B4_2422 0.61 -0.1 2,747,804 - Pf1N1B4_2422 0.61 +0.6 2,747,804 - Pf1N1B4_2422 0.61 +0.3 2,747,804 - Pf1N1B4_2422 0.61 +0.9 2,747,804 - Pf1N1B4_2422 0.61 +0.6 2,747,804 - Pf1N1B4_2422 0.61 -0.9 2,747,804 - Pf1N1B4_2422 0.61 -0.7 2,747,830 + Pf1N1B4_2422 0.64 -1.0 2,747,831 - Pf1N1B4_2422 0.64 -2.5 2,747,849 + Pf1N1B4_2422 0.66 +0.1 2,747,922 - Pf1N1B4_2422 0.74 +0.6 2,747,965 + Pf1N1B4_2422 0.78 +1.3 2,747,965 + Pf1N1B4_2422 0.78 +1.3 2,747,965 + Pf1N1B4_2422 0.78 +1.3 2,747,966 - Pf1N1B4_2422 0.78 +0.9 2,747,966 - Pf1N1B4_2422 0.78 -0.2 2,748,004 + Pf1N1B4_2422 0.82 +1.3 2,748,005 - Pf1N1B4_2422 0.83 +0.3
Or see this region's nucleotide sequence