Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2263 and Pf1N1B4_2264 are separated by 226 nucleotides Pf1N1B4_2264 and Pf1N1B4_2265 are separated by 103 nucleotides Pf1N1B4_2265 and Pf1N1B4_2266 are separated by 97 nucleotides
Pf1N1B4_2263 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 2,579,510 to 2,580,400
_2263
Pf1N1B4_2264 - Penicillin acylase II (EC 3.5.1.11), at 2,580,627 to 2,583,038
_2264
Pf1N1B4_2265 - Thiol-disulfide isomerase and thioredoxins, at 2,583,142 to 2,583,720
_2265
Pf1N1B4_2266 - Lysophospholipase (EC 3.1.1.5), at 2,583,818 to 2,584,771
_2266
Position (kb)
2580
2581
2582
2583
2584 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.649 kb on - strand, within Pf1N1B4_2263 at 2579.714 kb on + strand, within Pf1N1B4_2263 at 2579.770 kb on + strand, within Pf1N1B4_2263 at 2579.954 kb on + strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand at 2580.612 kb on + strand at 2580.613 kb on - strand at 2580.613 kb on - strand at 2580.628 kb on + strand at 2580.628 kb on + strand at 2580.628 kb on + strand at 2580.629 kb on - strand at 2580.629 kb on - strand at 2580.709 kb on + strand at 2580.709 kb on + strand at 2580.710 kb on - strand at 2580.710 kb on - strand at 2580.710 kb on - strand at 2580.712 kb on + strand at 2580.712 kb on + strand at 2580.713 kb on - strand at 2580.713 kb on - strand at 2580.713 kb on - strand at 2580.725 kb on + strand at 2580.727 kb on + strand at 2580.727 kb on + strand at 2580.727 kb on + strand at 2580.727 kb on + strand at 2580.727 kb on + strand at 2580.727 kb on + strand at 2580.728 kb on - strand at 2580.728 kb on - strand at 2580.728 kb on - strand at 2580.728 kb on - strand at 2580.748 kb on + strand at 2580.748 kb on + strand at 2580.748 kb on + strand at 2580.749 kb on - strand at 2580.847 kb on + strand at 2580.847 kb on + strand at 2580.847 kb on + strand at 2580.848 kb on - strand at 2580.848 kb on - strand at 2580.848 kb on - strand at 2580.848 kb on - strand at 2580.848 kb on - strand at 2580.848 kb on - strand at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.501 kb on + strand, within Pf1N1B4_2264 at 2581.502 kb on - strand, within Pf1N1B4_2264 at 2581.502 kb on - strand, within Pf1N1B4_2264 at 2581.502 kb on - strand, within Pf1N1B4_2264 at 2581.502 kb on - strand, within Pf1N1B4_2264 at 2581.502 kb on - strand, within Pf1N1B4_2264 at 2581.505 kb on - strand, within Pf1N1B4_2264 at 2581.561 kb on + strand, within Pf1N1B4_2264 at 2581.561 kb on + strand, within Pf1N1B4_2264 at 2581.562 kb on - strand, within Pf1N1B4_2264 at 2581.562 kb on - strand, within Pf1N1B4_2264 at 2581.562 kb on - strand, within Pf1N1B4_2264 at 2581.562 kb on - strand, within Pf1N1B4_2264 at 2581.562 kb on - strand, within Pf1N1B4_2264 at 2581.729 kb on + strand, within Pf1N1B4_2264 at 2581.730 kb on - strand, within Pf1N1B4_2264 at 2581.730 kb on - strand, within Pf1N1B4_2264 at 2581.811 kb on - strand, within Pf1N1B4_2264 at 2582.149 kb on + strand, within Pf1N1B4_2264 at 2582.150 kb on - strand, within Pf1N1B4_2264 at 2582.150 kb on - strand, within Pf1N1B4_2264 at 2582.150 kb on - strand, within Pf1N1B4_2264 at 2582.185 kb on + strand, within Pf1N1B4_2264 at 2582.186 kb on - strand, within Pf1N1B4_2264 at 2582.249 kb on + strand, within Pf1N1B4_2264 at 2582.249 kb on + strand, within Pf1N1B4_2264 at 2582.250 kb on - strand, within Pf1N1B4_2264 at 2582.406 kb on + strand, within Pf1N1B4_2264 at 2582.447 kb on - strand, within Pf1N1B4_2264 at 2582.473 kb on + strand, within Pf1N1B4_2264 at 2582.473 kb on + strand, within Pf1N1B4_2264 at 2582.474 kb on - strand, within Pf1N1B4_2264 at 2582.474 kb on - strand, within Pf1N1B4_2264 at 2582.474 kb on - strand, within Pf1N1B4_2264 at 2582.515 kb on + strand, within Pf1N1B4_2264 at 2582.516 kb on - strand, within Pf1N1B4_2264 at 2582.524 kb on + strand at 2582.618 kb on - strand, within Pf1N1B4_2264 at 2582.638 kb on + strand, within Pf1N1B4_2264 at 2582.689 kb on + strand, within Pf1N1B4_2264 at 2582.689 kb on + strand, within Pf1N1B4_2264 at 2582.690 kb on - strand, within Pf1N1B4_2264 at 2582.690 kb on - strand, within Pf1N1B4_2264 at 2582.690 kb on - strand, within Pf1N1B4_2264 at 2582.690 kb on - strand, within Pf1N1B4_2264 at 2582.711 kb on + strand, within Pf1N1B4_2264 at 2582.833 kb on + strand at 2582.833 kb on + strand at 2582.833 kb on + strand at 2582.834 kb on - strand at 2582.834 kb on - strand at 2582.834 kb on - strand at 2582.968 kb on + strand at 2582.968 kb on + strand at 2582.968 kb on + strand at 2582.968 kb on + strand at 2582.974 kb on + strand at 2582.974 kb on + strand at 2582.974 kb on + strand at 2582.974 kb on + strand at 2582.974 kb on + strand at 2582.974 kb on + strand at 2582.975 kb on - strand at 2582.975 kb on - strand at 2583.018 kb on + strand at 2583.018 kb on + strand at 2583.018 kb on + strand at 2583.018 kb on + strand at 2583.019 kb on - strand at 2583.019 kb on - strand at 2583.089 kb on + strand at 2583.143 kb on + strand at 2583.143 kb on + strand at 2583.143 kb on + strand at 2583.143 kb on + strand at 2583.143 kb on + strand at 2583.143 kb on + strand at 2583.144 kb on - strand at 2583.293 kb on + strand, within Pf1N1B4_2265 at 2583.293 kb on + strand, within Pf1N1B4_2265 at 2583.378 kb on + strand, within Pf1N1B4_2265 at 2583.379 kb on - strand, within Pf1N1B4_2265 at 2583.379 kb on - strand, within Pf1N1B4_2265 at 2583.379 kb on - strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.434 kb on + strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.435 kb on - strand, within Pf1N1B4_2265 at 2583.454 kb on + strand, within Pf1N1B4_2265 at 2583.454 kb on + strand, within Pf1N1B4_2265 at 2583.454 kb on + strand, within Pf1N1B4_2265 at 2583.455 kb on - strand, within Pf1N1B4_2265 at 2583.455 kb on - strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.458 kb on + strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.459 kb on - strand, within Pf1N1B4_2265 at 2583.624 kb on - strand, within Pf1N1B4_2265 at 2583.624 kb on - strand, within Pf1N1B4_2265 at 2583.624 kb on - strand, within Pf1N1B4_2265 at 2583.645 kb on - strand, within Pf1N1B4_2265 at 2583.655 kb on + strand, within Pf1N1B4_2265 at 2583.707 kb on + strand at 2583.746 kb on - strand at 2583.813 kb on - strand at 2583.905 kb on + strand at 2583.905 kb on + strand at 2583.906 kb on - strand at 2583.906 kb on - strand at 2583.917 kb on + strand, within Pf1N1B4_2266 at 2583.918 kb on - strand, within Pf1N1B4_2266 at 2583.980 kb on + strand, within Pf1N1B4_2266 at 2583.981 kb on - strand, within Pf1N1B4_2266
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,579,648 + Pf1N1B4_2263 0.15 -0.6 2,579,648 + Pf1N1B4_2263 0.15 +0.1 2,579,648 + Pf1N1B4_2263 0.15 -1.8 2,579,649 - Pf1N1B4_2263 0.16 +0.4 2,579,714 + Pf1N1B4_2263 0.23 -2.1 2,579,770 + Pf1N1B4_2263 0.29 +1.0 2,579,954 + Pf1N1B4_2263 0.50 +0.9 2,579,955 - Pf1N1B4_2263 0.50 +1.4 2,579,955 - Pf1N1B4_2263 0.50 -0.3 2,579,955 - Pf1N1B4_2263 0.50 -0.2 2,579,955 - Pf1N1B4_2263 0.50 +0.7 2,579,955 - +0.4 2,580,612 + +1.4 2,580,613 - -0.6 2,580,613 - +2.4 2,580,628 + -0.0 2,580,628 + +1.1 2,580,628 + +0.4 2,580,629 - -0.2 2,580,629 - -0.5 2,580,709 + -0.2 2,580,709 + -0.6 2,580,710 - +0.4 2,580,710 - -1.9 2,580,710 - -1.2 2,580,712 + +0.2 2,580,712 + +1.4 2,580,713 - -0.1 2,580,713 - +2.4 2,580,713 - +1.4 2,580,725 + -0.9 2,580,727 + +1.1 2,580,727 + -1.9 2,580,727 + -1.2 2,580,727 + -0.3 2,580,727 + -0.6 2,580,727 + -1.2 2,580,728 - -1.2 2,580,728 - -2.6 2,580,728 - +1.4 2,580,728 - -0.6 2,580,748 + +0.4 2,580,748 + +0.4 2,580,748 + -2.0 2,580,749 - -1.6 2,580,847 + +1.0 2,580,847 + -0.6 2,580,847 + -0.7 2,580,848 - -1.4 2,580,848 - -1.3 2,580,848 - -0.4 2,580,848 - -0.6 2,580,848 - -0.6 2,580,848 - +0.5 2,581,501 + Pf1N1B4_2264 0.36 -1.9 2,581,501 + Pf1N1B4_2264 0.36 +0.4 2,581,501 + Pf1N1B4_2264 0.36 +1.0 2,581,501 + Pf1N1B4_2264 0.36 +0.8 2,581,501 + Pf1N1B4_2264 0.36 +0.4 2,581,501 + Pf1N1B4_2264 0.36 +0.4 2,581,502 - Pf1N1B4_2264 0.36 +0.4 2,581,502 - Pf1N1B4_2264 0.36 +2.4 2,581,502 - Pf1N1B4_2264 0.36 +0.1 2,581,502 - Pf1N1B4_2264 0.36 -0.3 2,581,502 - Pf1N1B4_2264 0.36 -0.6 2,581,505 - Pf1N1B4_2264 0.36 +0.4 2,581,561 + Pf1N1B4_2264 0.39 +0.9 2,581,561 + Pf1N1B4_2264 0.39 +0.1 2,581,562 - Pf1N1B4_2264 0.39 +0.4 2,581,562 - Pf1N1B4_2264 0.39 -0.3 2,581,562 - Pf1N1B4_2264 0.39 +1.2 2,581,562 - Pf1N1B4_2264 0.39 -0.0 2,581,562 - Pf1N1B4_2264 0.39 +0.9 2,581,729 + Pf1N1B4_2264 0.46 -0.6 2,581,730 - Pf1N1B4_2264 0.46 +0.3 2,581,730 - Pf1N1B4_2264 0.46 -0.8 2,581,811 - Pf1N1B4_2264 0.49 +1.0 2,582,149 + Pf1N1B4_2264 0.63 +0.4 2,582,150 - Pf1N1B4_2264 0.63 +1.1 2,582,150 - Pf1N1B4_2264 0.63 -1.9 2,582,150 - Pf1N1B4_2264 0.63 +0.7 2,582,185 + Pf1N1B4_2264 0.65 -1.2 2,582,186 - Pf1N1B4_2264 0.65 -1.8 2,582,249 + Pf1N1B4_2264 0.67 +1.0 2,582,249 + Pf1N1B4_2264 0.67 +0.4 2,582,250 - Pf1N1B4_2264 0.67 +0.1 2,582,406 + Pf1N1B4_2264 0.74 +1.0 2,582,447 - Pf1N1B4_2264 0.75 +2.2 2,582,473 + Pf1N1B4_2264 0.77 +0.4 2,582,473 + Pf1N1B4_2264 0.77 -1.6 2,582,474 - Pf1N1B4_2264 0.77 +1.2 2,582,474 - Pf1N1B4_2264 0.77 +0.4 2,582,474 - Pf1N1B4_2264 0.77 +1.0 2,582,515 + Pf1N1B4_2264 0.78 +1.0 2,582,516 - Pf1N1B4_2264 0.78 +1.2 2,582,524 + +2.0 2,582,618 - Pf1N1B4_2264 0.83 -0.6 2,582,638 + Pf1N1B4_2264 0.83 +0.4 2,582,689 + Pf1N1B4_2264 0.85 -0.6 2,582,689 + Pf1N1B4_2264 0.85 +2.0 2,582,690 - Pf1N1B4_2264 0.86 +0.4 2,582,690 - Pf1N1B4_2264 0.86 +1.0 2,582,690 - Pf1N1B4_2264 0.86 +0.8 2,582,690 - Pf1N1B4_2264 0.86 -0.3 2,582,711 + Pf1N1B4_2264 0.86 +0.1 2,582,833 + -2.4 2,582,833 + +0.8 2,582,833 + +1.0 2,582,834 - +0.7 2,582,834 - -0.8 2,582,834 - -0.6 2,582,968 + -1.9 2,582,968 + +0.4 2,582,968 + +1.1 2,582,968 + +1.0 2,582,974 + -0.8 2,582,974 + +0.1 2,582,974 + -1.2 2,582,974 + -0.2 2,582,974 + -1.2 2,582,974 + -0.1 2,582,975 - -0.0 2,582,975 - +0.7 2,583,018 + -0.6 2,583,018 + +0.1 2,583,018 + +0.4 2,583,018 + -0.8 2,583,019 - -0.1 2,583,019 - +0.7 2,583,089 + +1.0 2,583,143 + -1.2 2,583,143 + +1.1 2,583,143 + -1.2 2,583,143 + -1.2 2,583,143 + -0.0 2,583,143 + -0.2 2,583,144 - -1.6 2,583,293 + Pf1N1B4_2265 0.26 +0.1 2,583,293 + Pf1N1B4_2265 0.26 -0.0 2,583,378 + Pf1N1B4_2265 0.41 -0.3 2,583,379 - Pf1N1B4_2265 0.41 +0.6 2,583,379 - Pf1N1B4_2265 0.41 +0.6 2,583,379 - Pf1N1B4_2265 0.41 -1.1 2,583,434 + Pf1N1B4_2265 0.50 +2.0 2,583,434 + Pf1N1B4_2265 0.50 -0.2 2,583,434 + Pf1N1B4_2265 0.50 -1.9 2,583,434 + Pf1N1B4_2265 0.50 +2.0 2,583,434 + Pf1N1B4_2265 0.50 -0.6 2,583,434 + Pf1N1B4_2265 0.50 +0.4 2,583,434 + Pf1N1B4_2265 0.50 -0.5 2,583,435 - Pf1N1B4_2265 0.51 -0.6 2,583,435 - Pf1N1B4_2265 0.51 -0.0 2,583,435 - Pf1N1B4_2265 0.51 +0.9 2,583,435 - Pf1N1B4_2265 0.51 -0.0 2,583,435 - Pf1N1B4_2265 0.51 +0.5 2,583,435 - Pf1N1B4_2265 0.51 +1.2 2,583,435 - Pf1N1B4_2265 0.51 -0.2 2,583,454 + Pf1N1B4_2265 0.54 +0.6 2,583,454 + Pf1N1B4_2265 0.54 -0.6 2,583,454 + Pf1N1B4_2265 0.54 +1.1 2,583,455 - Pf1N1B4_2265 0.54 -1.6 2,583,455 - Pf1N1B4_2265 0.54 +0.6 2,583,458 + Pf1N1B4_2265 0.55 +1.1 2,583,458 + Pf1N1B4_2265 0.55 +0.1 2,583,458 + Pf1N1B4_2265 0.55 -2.6 2,583,458 + Pf1N1B4_2265 0.55 +0.1 2,583,458 + Pf1N1B4_2265 0.55 +0.4 2,583,458 + Pf1N1B4_2265 0.55 +0.9 2,583,458 + Pf1N1B4_2265 0.55 +2.1 2,583,458 + Pf1N1B4_2265 0.55 -0.1 2,583,458 + Pf1N1B4_2265 0.55 -0.7 2,583,458 + Pf1N1B4_2265 0.55 +0.4 2,583,458 + Pf1N1B4_2265 0.55 +1.4 2,583,459 - Pf1N1B4_2265 0.55 -0.6 2,583,459 - Pf1N1B4_2265 0.55 -0.6 2,583,459 - +0.4 2,583,459 - Pf1N1B4_2265 0.55 +0.4 2,583,459 - Pf1N1B4_2265 0.55 +0.6 2,583,459 - Pf1N1B4_2265 0.55 +0.7 2,583,459 - Pf1N1B4_2265 0.55 -0.9 2,583,459 - Pf1N1B4_2265 0.55 +0.7 2,583,459 - Pf1N1B4_2265 0.55 -0.2 2,583,624 - Pf1N1B4_2265 0.83 -0.8 2,583,624 - Pf1N1B4_2265 0.83 -0.5 2,583,624 - Pf1N1B4_2265 0.83 +0.6 2,583,645 - Pf1N1B4_2265 0.87 -0.6 2,583,655 + Pf1N1B4_2265 0.89 -0.9 2,583,707 + -0.0 2,583,746 - +0.2 2,583,813 - +1.4 2,583,905 + -0.6 2,583,905 + -0.5 2,583,906 - +0.8 2,583,906 - -0.6 2,583,917 + Pf1N1B4_2266 0.10 -0.2 2,583,918 - Pf1N1B4_2266 0.10 -0.2 2,583,980 + Pf1N1B4_2266 0.17 +1.1 2,583,981 - Pf1N1B4_2266 0.17 +1.1
Or see this region's nucleotide sequence