Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2249 and Pf1N1B4_2250 are separated by 9 nucleotides Pf1N1B4_2250 and Pf1N1B4_2251 overlap by 4 nucleotides Pf1N1B4_2251 and Pf1N1B4_2252 are separated by 34 nucleotides
Pf1N1B4_2249 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,565,573 to 2,566,721
_2249
Pf1N1B4_2250 - Alkanesulfonates transport system permease protein, at 2,566,731 to 2,567,513
_2250
Pf1N1B4_2251 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 2,567,510 to 2,568,316
_2251
Pf1N1B4_2252 - Organosulfonate utilization protein SsuF, at 2,568,351 to 2,568,566
_2252
Position (kb)
2566
2567
2568 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2566.020 kb on + strand, within Pf1N1B4_2249 at 2566.021 kb on - strand, within Pf1N1B4_2249 at 2566.063 kb on - strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.547 kb on + strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.548 kb on - strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.606 kb on + strand, within Pf1N1B4_2249 at 2566.607 kb on - strand at 2566.657 kb on - strand at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.961 kb on + strand, within Pf1N1B4_2250 at 2566.962 kb on - strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.136 kb on + strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.137 kb on - strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.148 kb on + strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.149 kb on - strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.199 kb on + strand, within Pf1N1B4_2250 at 2567.200 kb on - strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.409 kb on + strand, within Pf1N1B4_2250 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.671 kb on + strand, within Pf1N1B4_2251 at 2567.672 kb on - strand, within Pf1N1B4_2251 at 2568.293 kb on - strand at 2568.314 kb on + strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.315 kb on - strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.319 kb on + strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.320 kb on - strand at 2568.356 kb on + strand at 2568.357 kb on - strand at 2568.357 kb on - strand at 2568.378 kb on - strand, within Pf1N1B4_2252 at 2568.474 kb on - strand, within Pf1N1B4_2252
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,566,020 + Pf1N1B4_2249 0.39 +2.3 2,566,021 - Pf1N1B4_2249 0.39 -1.3 2,566,063 - Pf1N1B4_2249 0.43 -0.6 2,566,547 + Pf1N1B4_2249 0.85 -0.0 2,566,547 + Pf1N1B4_2249 0.85 -0.8 2,566,547 + Pf1N1B4_2249 0.85 +0.2 2,566,548 - Pf1N1B4_2249 0.85 -0.3 2,566,548 - Pf1N1B4_2249 0.85 +0.4 2,566,606 + Pf1N1B4_2249 0.90 -0.6 2,566,606 + Pf1N1B4_2249 0.90 -1.2 2,566,607 - +1.0 2,566,657 - -0.4 2,566,961 + Pf1N1B4_2250 0.29 -1.2 2,566,961 + Pf1N1B4_2250 0.29 -0.6 2,566,961 + Pf1N1B4_2250 0.29 -2.4 2,566,961 + Pf1N1B4_2250 0.29 +0.7 2,566,961 + Pf1N1B4_2250 0.29 +0.4 2,566,961 + Pf1N1B4_2250 0.29 -2.2 2,566,961 + Pf1N1B4_2250 0.29 -0.6 2,566,962 - Pf1N1B4_2250 0.30 -1.9 2,567,136 + Pf1N1B4_2250 0.52 -0.6 2,567,136 + Pf1N1B4_2250 0.52 +1.4 2,567,137 - Pf1N1B4_2250 0.52 -0.6 2,567,137 - Pf1N1B4_2250 0.52 -0.9 2,567,148 + Pf1N1B4_2250 0.53 -0.6 2,567,148 + Pf1N1B4_2250 0.53 -0.6 2,567,148 + Pf1N1B4_2250 0.53 -0.4 2,567,148 + Pf1N1B4_2250 0.53 -1.6 2,567,148 + Pf1N1B4_2250 0.53 -0.6 2,567,148 + Pf1N1B4_2250 0.53 -1.6 2,567,148 + Pf1N1B4_2250 0.53 -0.9 2,567,149 - Pf1N1B4_2250 0.53 -0.3 2,567,149 - Pf1N1B4_2250 0.53 -0.2 2,567,149 - Pf1N1B4_2250 0.53 +0.2 2,567,149 - Pf1N1B4_2250 0.53 -0.0 2,567,199 + Pf1N1B4_2250 0.60 -0.6 2,567,199 + Pf1N1B4_2250 0.60 -1.2 2,567,199 + Pf1N1B4_2250 0.60 -2.2 2,567,200 - Pf1N1B4_2250 0.60 +0.4 2,567,409 + Pf1N1B4_2250 0.87 -0.3 2,567,409 + Pf1N1B4_2250 0.87 -0.6 2,567,409 + Pf1N1B4_2250 0.87 +1.2 2,567,671 + Pf1N1B4_2251 0.20 -1.2 2,567,671 + Pf1N1B4_2251 0.20 -0.9 2,567,672 - Pf1N1B4_2251 0.20 +0.9 2,568,293 - +0.7 2,568,314 + +0.9 2,568,315 - +0.8 2,568,315 - -0.0 2,568,315 - -0.9 2,568,319 + +0.4 2,568,319 + -1.9 2,568,319 + -2.4 2,568,320 - -2.2 2,568,320 - -0.2 2,568,320 - +0.1 2,568,320 - -1.6 2,568,320 - -0.6 2,568,356 + +0.4 2,568,357 - +3.0 2,568,357 - +1.4 2,568,378 - Pf1N1B4_2252 0.12 +0.2 2,568,474 - Pf1N1B4_2252 0.57 -0.1
Or see this region's nucleotide sequence