Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_175 and Pf1N1B4_176 are separated by 247 nucleotides Pf1N1B4_176 and Pf1N1B4_177 are separated by 151 nucleotides
Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076
_175
Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349
_176
Pf1N1B4_177 - N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87), at 220,501 to 221,730
_177
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 218.388 kb on + strand, within Pf1N1B4_175 at 218.563 kb on - strand at 218.608 kb on - strand, within Pf1N1B4_175 at 218.608 kb on - strand, within Pf1N1B4_175 at 218.981 kb on + strand at 218.981 kb on + strand at 219.044 kb on + strand at 219.044 kb on + strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.069 kb on - strand at 219.169 kb on - strand at 219.169 kb on - strand at 219.306 kb on + strand at 219.307 kb on - strand at 219.507 kb on - strand, within Pf1N1B4_176 at 219.718 kb on + strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.848 kb on - strand, within Pf1N1B4_176 at 219.908 kb on - strand, within Pf1N1B4_176 at 220.021 kb on + strand, within Pf1N1B4_176 at 220.022 kb on - strand, within Pf1N1B4_176 at 220.153 kb on + strand, within Pf1N1B4_176 at 220.277 kb on + strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.298 kb on - strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.300 kb on + strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.301 kb on - strand at 220.358 kb on + strand at 220.358 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.374 kb on + strand at 220.375 kb on - strand at 220.503 kb on - strand at 220.782 kb on + strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.783 kb on - strand, within Pf1N1B4_177 at 220.929 kb on + strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 220.930 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand at 221.053 kb on - strand, within Pf1N1B4_177 at 221.053 kb on - strand, within Pf1N1B4_177 at 221.183 kb on + strand, within Pf1N1B4_177 at 221.184 kb on - strand at 221.184 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 218,388 + Pf1N1B4_175 0.64 -1.4 218,563 - -2.3 218,608 - Pf1N1B4_175 0.75 -3.1 218,608 - Pf1N1B4_175 0.75 -1.9 218,981 + +0.4 218,981 + +0.2 219,044 + -2.3 219,044 + +2.7 219,045 - +0.7 219,045 - -1.3 219,045 - -0.1 219,069 - +1.6 219,169 - -2.3 219,169 - +1.2 219,306 + -2.3 219,307 - -1.0 219,507 - Pf1N1B4_176 0.18 -1.6 219,718 + Pf1N1B4_176 0.38 -1.5 219,719 - Pf1N1B4_176 0.38 -2.3 219,719 - Pf1N1B4_176 0.38 -1.9 219,719 - Pf1N1B4_176 0.38 +0.7 219,847 + Pf1N1B4_176 0.51 -0.3 219,847 + Pf1N1B4_176 0.51 +2.1 219,848 - Pf1N1B4_176 0.51 -0.7 219,908 - Pf1N1B4_176 0.57 -0.6 220,021 + Pf1N1B4_176 0.68 -0.3 220,022 - Pf1N1B4_176 0.68 +0.1 220,153 + Pf1N1B4_176 0.81 -0.7 220,277 + -1.3 220,298 - +0.2 220,298 - -0.9 220,298 - -1.9 220,300 + -0.8 220,300 + +0.3 220,300 + -0.9 220,300 + +0.4 220,300 + +0.3 220,300 + -1.3 220,301 - -1.3 220,301 - -0.3 220,301 - -2.3 220,358 + -0.3 220,358 + +0.2 220,374 + -1.0 220,374 + -0.0 220,374 + -1.3 220,374 + -0.6 220,374 + +0.7 220,375 - +1.7 220,503 - +1.2 220,782 + Pf1N1B4_177 0.23 +0.5 220,783 - Pf1N1B4_177 0.23 +0.7 220,783 - Pf1N1B4_177 0.23 -0.9 220,929 + Pf1N1B4_177 0.35 -0.8 220,930 - Pf1N1B4_177 0.35 -0.1 220,930 - Pf1N1B4_177 0.35 -0.3 220,930 - Pf1N1B4_177 0.35 +0.7 220,930 - Pf1N1B4_177 0.35 -1.4 221,053 - +0.7 221,053 - Pf1N1B4_177 0.45 +2.5 221,053 - Pf1N1B4_177 0.45 -1.3 221,183 + Pf1N1B4_177 0.55 +0.7 221,184 - -0.3 221,184 - +0.7
Or see this region's nucleotide sequence