Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1010 and Pf1N1B4_1011 are separated by 143 nucleotides Pf1N1B4_1011 and Pf1N1B4_1012 are separated by 216 nucleotides Pf1N1B4_1012 and Pf1N1B4_1013 are separated by 135 nucleotides
Pf1N1B4_1010 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 1,144,523 to 1,146,856
_1010
Pf1N1B4_1011 - ribosomal subunit interface protein, putative, at 1,147,000 to 1,147,407
_1011
Pf1N1B4_1012 - Glycine cleavage system transcriptional activator, at 1,147,624 to 1,148,571
_1012
Pf1N1B4_1013 - L-serine ammonia-lyase (EC 4.3.1.17) (from data) , at 1,148,707 to 1,150,083
_1013
Position (kb)
1147
1148
1149 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.671 kb on + strand at 1146.672 kb on - strand at 1146.672 kb on - strand at 1146.679 kb on - strand at 1146.698 kb on + strand at 1146.698 kb on + strand at 1146.698 kb on + strand at 1146.699 kb on - strand at 1146.699 kb on - strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.767 kb on + strand at 1146.768 kb on - strand at 1146.768 kb on - strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.806 kb on + strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.807 kb on - strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.830 kb on + strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.831 kb on - strand at 1146.923 kb on + strand at 1146.923 kb on + strand at 1146.924 kb on - strand at 1146.943 kb on + strand at 1146.944 kb on - strand at 1146.984 kb on - strand at 1146.984 kb on - strand at 1146.984 kb on - strand at 1146.989 kb on + strand at 1146.989 kb on + strand at 1146.990 kb on - strand at 1146.990 kb on - strand at 1147.186 kb on - strand, within Pf1N1B4_1011 at 1147.314 kb on - strand, within Pf1N1B4_1011 at 1147.314 kb on - strand, within Pf1N1B4_1011 at 1147.338 kb on + strand, within Pf1N1B4_1011 at 1147.338 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.341 kb on + strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.342 kb on - strand, within Pf1N1B4_1011 at 1147.346 kb on - strand, within Pf1N1B4_1011 at 1147.377 kb on + strand at 1147.407 kb on + strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.408 kb on - strand at 1147.420 kb on - strand at 1147.420 kb on - strand at 1147.420 kb on - strand at 1147.429 kb on - strand at 1147.429 kb on - strand at 1147.429 kb on - strand at 1147.536 kb on - strand at 1147.578 kb on + strand at 1147.656 kb on + strand at 1147.657 kb on - strand at 1147.657 kb on - strand at 1147.664 kb on - strand at 1147.664 kb on - strand at 1147.664 kb on - strand at 1147.866 kb on + strand, within Pf1N1B4_1012 at 1147.890 kb on + strand, within Pf1N1B4_1012 at 1147.891 kb on - strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.937 kb on + strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.938 kb on - strand, within Pf1N1B4_1012 at 1147.956 kb on - strand, within Pf1N1B4_1012 at 1147.961 kb on + strand, within Pf1N1B4_1012 at 1147.962 kb on - strand, within Pf1N1B4_1012 at 1148.071 kb on + strand at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand, within Pf1N1B4_1012 at 1148.072 kb on - strand at 1148.423 kb on - strand, within Pf1N1B4_1012 at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.543 kb on + strand at 1148.544 kb on + strand at 1148.544 kb on - strand at 1148.597 kb on + strand at 1148.694 kb on + strand at 1148.697 kb on - strand at 1148.857 kb on + strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.019 kb on - strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.070 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.133 kb on + strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.134 kb on - strand, within Pf1N1B4_1013 at 1149.136 kb on + strand, within Pf1N1B4_1013 at 1149.137 kb on - strand, within Pf1N1B4_1013 at 1149.218 kb on - strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.345 kb on + strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.346 kb on - strand, within Pf1N1B4_1013 at 1149.530 kb on + strand, within Pf1N1B4_1013
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,146,671 + +0.5 1,146,671 + -1.2 1,146,671 + +0.9 1,146,671 + -0.5 1,146,671 + +1.1 1,146,672 - -0.5 1,146,672 - -0.5 1,146,679 - -0.2 1,146,698 + +0.8 1,146,698 + +0.3 1,146,698 + -0.5 1,146,699 - -0.5 1,146,699 - +0.9 1,146,767 + +0.1 1,146,767 + +0.5 1,146,767 + -0.1 1,146,767 + +0.5 1,146,767 + +0.6 1,146,767 + +1.0 1,146,768 - +0.7 1,146,768 - -0.3 1,146,806 + +0.7 1,146,806 + -0.4 1,146,806 + -0.3 1,146,806 + +0.5 1,146,806 + +3.3 1,146,806 + +0.3 1,146,806 + +1.1 1,146,806 + +1.4 1,146,807 - -0.8 1,146,807 - +1.7 1,146,807 - +0.1 1,146,807 - +0.5 1,146,807 - +0.8 1,146,830 + +1.4 1,146,830 + +0.1 1,146,830 + +1.1 1,146,830 + +0.1 1,146,830 + +0.1 1,146,830 + +0.7 1,146,830 + -0.9 1,146,830 + -0.0 1,146,830 + +0.1 1,146,830 + +1.1 1,146,830 + +0.1 1,146,830 + -1.5 1,146,830 + +0.7 1,146,830 + -0.6 1,146,830 + -0.9 1,146,831 - -0.9 1,146,831 - +0.8 1,146,831 - -0.1 1,146,831 - +1.1 1,146,831 - -0.1 1,146,831 - +0.1 1,146,831 - +0.9 1,146,831 - +0.1 1,146,831 - -0.2 1,146,831 - +0.4 1,146,923 + -1.5 1,146,923 + -0.4 1,146,924 - +1.1 1,146,943 + +0.1 1,146,944 - -0.9 1,146,984 - -0.9 1,146,984 - -0.9 1,146,984 - -1.5 1,146,989 + -0.9 1,146,989 + -0.5 1,146,990 - +0.1 1,146,990 - -2.4 1,147,186 - Pf1N1B4_1011 0.46 -0.6 1,147,314 - Pf1N1B4_1011 0.77 -1.7 1,147,314 - Pf1N1B4_1011 0.77 -0.5 1,147,338 + Pf1N1B4_1011 0.83 +2.1 1,147,338 + Pf1N1B4_1011 0.83 -0.5 1,147,341 + Pf1N1B4_1011 0.84 +1.4 1,147,341 + Pf1N1B4_1011 0.84 +1.1 1,147,341 + Pf1N1B4_1011 0.84 -1.9 1,147,341 + Pf1N1B4_1011 0.84 +1.1 1,147,341 + Pf1N1B4_1011 0.84 -0.6 1,147,341 + Pf1N1B4_1011 0.84 +0.1 1,147,342 - Pf1N1B4_1011 0.84 +0.4 1,147,342 - Pf1N1B4_1011 0.84 -0.5 1,147,342 - +0.1 1,147,342 - Pf1N1B4_1011 0.84 -0.3 1,147,342 - Pf1N1B4_1011 0.84 -1.5 1,147,342 - Pf1N1B4_1011 0.84 +0.7 1,147,346 - Pf1N1B4_1011 0.85 -1.3 1,147,377 + +0.7 1,147,407 + -0.3 1,147,408 - -0.5 1,147,408 - +1.4 1,147,408 - -0.5 1,147,408 - +0.3 1,147,408 - -0.2 1,147,408 - +0.7 1,147,408 - +0.1 1,147,408 - +2.1 1,147,420 - -0.9 1,147,420 - +0.1 1,147,420 - -0.5 1,147,429 - +0.1 1,147,429 - -1.0 1,147,429 - +1.1 1,147,536 - -1.2 1,147,578 + -1.5 1,147,656 + +1.1 1,147,657 - -0.3 1,147,657 - +0.9 1,147,664 - -0.9 1,147,664 - +0.1 1,147,664 - +0.1 1,147,866 + Pf1N1B4_1012 0.26 +0.1 1,147,890 + Pf1N1B4_1012 0.28 +1.1 1,147,891 - Pf1N1B4_1012 0.28 -2.7 1,147,937 + Pf1N1B4_1012 0.33 +0.4 1,147,937 + Pf1N1B4_1012 0.33 +1.1 1,147,937 + Pf1N1B4_1012 0.33 -0.8 1,147,938 - Pf1N1B4_1012 0.33 -0.9 1,147,938 - Pf1N1B4_1012 0.33 +1.7 1,147,938 - Pf1N1B4_1012 0.33 -0.6 1,147,956 - Pf1N1B4_1012 0.35 -1.9 1,147,961 + Pf1N1B4_1012 0.36 -0.9 1,147,962 - Pf1N1B4_1012 0.36 +0.3 1,148,071 + +0.1 1,148,072 - Pf1N1B4_1012 0.47 -0.6 1,148,072 - Pf1N1B4_1012 0.47 -0.6 1,148,072 - +0.7 1,148,423 - Pf1N1B4_1012 0.84 -0.5 1,148,543 + -0.3 1,148,543 + +1.7 1,148,543 + -1.9 1,148,543 + -0.5 1,148,543 + +0.7 1,148,543 + +1.7 1,148,543 + +0.4 1,148,543 + +0.1 1,148,543 + -0.5 1,148,544 + -1.3 1,148,544 - -1.2 1,148,597 + +0.1 1,148,694 + +0.1 1,148,697 - +0.7 1,148,857 + Pf1N1B4_1013 0.11 -0.2 1,149,019 - Pf1N1B4_1013 0.23 -0.6 1,149,019 - Pf1N1B4_1013 0.23 +0.7 1,149,070 + Pf1N1B4_1013 0.26 +0.8 1,149,070 + Pf1N1B4_1013 0.26 -0.5 1,149,133 + Pf1N1B4_1013 0.31 -0.6 1,149,133 + Pf1N1B4_1013 0.31 -0.9 1,149,133 + Pf1N1B4_1013 0.31 -0.9 1,149,133 + Pf1N1B4_1013 0.31 +0.4 1,149,133 + Pf1N1B4_1013 0.31 +1.1 1,149,133 + Pf1N1B4_1013 0.31 -0.9 1,149,133 + Pf1N1B4_1013 0.31 -0.1 1,149,133 + Pf1N1B4_1013 0.31 -1.9 1,149,133 + Pf1N1B4_1013 0.31 -0.2 1,149,133 + Pf1N1B4_1013 0.31 -0.4 1,149,133 + Pf1N1B4_1013 0.31 -0.7 1,149,133 + Pf1N1B4_1013 0.31 +0.1 1,149,133 + Pf1N1B4_1013 0.31 +0.8 1,149,134 - Pf1N1B4_1013 0.31 -0.3 1,149,134 - Pf1N1B4_1013 0.31 -1.5 1,149,134 - Pf1N1B4_1013 0.31 -1.1 1,149,134 - Pf1N1B4_1013 0.31 -1.2 1,149,134 - Pf1N1B4_1013 0.31 -0.9 1,149,134 - Pf1N1B4_1013 0.31 +1.1 1,149,134 - Pf1N1B4_1013 0.31 +0.4 1,149,136 + Pf1N1B4_1013 0.31 -0.5 1,149,137 - Pf1N1B4_1013 0.31 +0.7 1,149,218 - Pf1N1B4_1013 0.37 -1.1 1,149,345 + Pf1N1B4_1013 0.46 -2.2 1,149,345 + Pf1N1B4_1013 0.46 -1.1 1,149,345 + Pf1N1B4_1013 0.46 -0.3 1,149,345 + Pf1N1B4_1013 0.46 +0.5 1,149,345 + Pf1N1B4_1013 0.46 +0.7 1,149,345 + Pf1N1B4_1013 0.46 -0.6 1,149,345 + Pf1N1B4_1013 0.46 +0.7 1,149,345 + Pf1N1B4_1013 0.46 -0.5 1,149,345 + Pf1N1B4_1013 0.46 -2.2 1,149,345 + Pf1N1B4_1013 0.46 -2.2 1,149,346 - Pf1N1B4_1013 0.46 -2.2 1,149,346 - Pf1N1B4_1013 0.46 -1.5 1,149,346 - Pf1N1B4_1013 0.46 +0.3 1,149,530 + Pf1N1B4_1013 0.60 -0.6
Or see this region's nucleotide sequence