Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_734 and Pf6N2E2_735 are separated by 277 nucleotides Pf6N2E2_735 and Pf6N2E2_736 are separated by 31 nucleotides Pf6N2E2_736 and Pf6N2E2_737 are separated by 5 nucleotides Pf6N2E2_737 and Pf6N2E2_738 are separated by 110 nucleotides
Pf6N2E2_734 - Molybdenum cofactor biosynthesis protein MoaA, at 841,985 to 842,977
_734
Pf6N2E2_735 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 843,255 to 845,270
_735
Pf6N2E2_736 - hypothetical protein, at 845,302 to 845,463
_736
Pf6N2E2_737 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 845,469 to 846,155
_737
Pf6N2E2_738 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 846,266 to 847,885
_738
Position (kb)
843
844
845
846 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 842.517 kb on - strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.684 kb on + strand, within Pf6N2E2_734 at 842.685 kb on - strand, within Pf6N2E2_734 at 842.975 kb on + strand at 842.981 kb on + strand at 842.981 kb on + strand at 842.981 kb on + strand at 842.981 kb on + strand at 843.088 kb on + strand at 843.088 kb on + strand at 843.088 kb on + strand at 843.088 kb on + strand at 843.089 kb on - strand at 843.128 kb on - strand at 843.128 kb on - strand at 843.233 kb on - strand at 843.234 kb on + strand at 843.234 kb on + strand at 843.235 kb on - strand at 843.235 kb on - strand at 843.235 kb on - strand at 843.238 kb on + strand at 843.238 kb on + strand at 843.238 kb on + strand at 843.238 kb on + strand at 843.239 kb on - strand at 843.239 kb on - strand at 843.239 kb on - strand at 843.239 kb on - strand at 843.239 kb on - strand at 843.239 kb on - strand at 843.285 kb on - strand at 843.519 kb on + strand, within Pf6N2E2_735 at 843.519 kb on + strand, within Pf6N2E2_735 at 843.519 kb on + strand, within Pf6N2E2_735 at 843.520 kb on - strand, within Pf6N2E2_735 at 843.520 kb on - strand, within Pf6N2E2_735 at 843.648 kb on + strand, within Pf6N2E2_735 at 843.676 kb on + strand, within Pf6N2E2_735 at 843.699 kb on + strand, within Pf6N2E2_735 at 843.699 kb on + strand, within Pf6N2E2_735 at 843.699 kb on + strand, within Pf6N2E2_735 at 843.700 kb on - strand, within Pf6N2E2_735 at 843.717 kb on + strand, within Pf6N2E2_735 at 843.717 kb on + strand, within Pf6N2E2_735 at 843.718 kb on - strand, within Pf6N2E2_735 at 843.718 kb on - strand, within Pf6N2E2_735 at 843.762 kb on - strand, within Pf6N2E2_735 at 843.967 kb on - strand, within Pf6N2E2_735 at 843.967 kb on - strand, within Pf6N2E2_735 at 843.967 kb on - strand, within Pf6N2E2_735 at 843.967 kb on - strand, within Pf6N2E2_735 at 843.967 kb on - strand, within Pf6N2E2_735 at 843.978 kb on + strand, within Pf6N2E2_735 at 843.978 kb on + strand, within Pf6N2E2_735 at 843.978 kb on + strand, within Pf6N2E2_735 at 843.978 kb on + strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand at 843.979 kb on - strand, within Pf6N2E2_735 at 843.979 kb on - strand, within Pf6N2E2_735 at 844.039 kb on - strand, within Pf6N2E2_735 at 844.039 kb on - strand, within Pf6N2E2_735 at 844.039 kb on - strand, within Pf6N2E2_735 at 844.039 kb on - strand, within Pf6N2E2_735 at 844.039 kb on - strand, within Pf6N2E2_735 at 844.051 kb on + strand, within Pf6N2E2_735 at 844.168 kb on - strand, within Pf6N2E2_735 at 844.209 kb on + strand, within Pf6N2E2_735 at 844.210 kb on - strand, within Pf6N2E2_735 at 844.210 kb on - strand, within Pf6N2E2_735 at 844.210 kb on - strand, within Pf6N2E2_735 at 844.210 kb on - strand, within Pf6N2E2_735 at 844.272 kb on + strand, within Pf6N2E2_735 at 844.273 kb on - strand, within Pf6N2E2_735 at 844.273 kb on - strand, within Pf6N2E2_735 at 844.273 kb on - strand, within Pf6N2E2_735 at 844.530 kb on + strand, within Pf6N2E2_735 at 844.530 kb on + strand, within Pf6N2E2_735 at 844.531 kb on - strand, within Pf6N2E2_735 at 844.531 kb on - strand, within Pf6N2E2_735 at 844.531 kb on - strand, within Pf6N2E2_735 at 844.645 kb on - strand, within Pf6N2E2_735 at 844.650 kb on + strand, within Pf6N2E2_735 at 844.845 kb on + strand, within Pf6N2E2_735 at 844.846 kb on - strand, within Pf6N2E2_735 at 844.846 kb on - strand, within Pf6N2E2_735 at 844.846 kb on - strand, within Pf6N2E2_735 at 844.950 kb on + strand, within Pf6N2E2_735 at 844.950 kb on + strand, within Pf6N2E2_735 at 844.950 kb on + strand, within Pf6N2E2_735 at 844.950 kb on + strand, within Pf6N2E2_735 at 844.950 kb on + strand, within Pf6N2E2_735 at 844.951 kb on - strand, within Pf6N2E2_735 at 844.953 kb on + strand, within Pf6N2E2_735 at 844.954 kb on - strand, within Pf6N2E2_735 at 844.954 kb on - strand, within Pf6N2E2_735 at 845.014 kb on + strand, within Pf6N2E2_735 at 845.014 kb on + strand, within Pf6N2E2_735 at 845.015 kb on - strand, within Pf6N2E2_735 at 845.037 kb on + strand, within Pf6N2E2_735 at 845.037 kb on + strand, within Pf6N2E2_735 at 845.038 kb on - strand, within Pf6N2E2_735 at 845.038 kb on - strand, within Pf6N2E2_735 at 845.064 kb on + strand, within Pf6N2E2_735 at 845.064 kb on + strand, within Pf6N2E2_735 at 845.064 kb on + strand, within Pf6N2E2_735 at 845.064 kb on + strand, within Pf6N2E2_735 at 845.064 kb on + strand, within Pf6N2E2_735 at 845.065 kb on - strand, within Pf6N2E2_735 at 845.065 kb on - strand, within Pf6N2E2_735 at 845.065 kb on - strand, within Pf6N2E2_735 at 845.065 kb on - strand, within Pf6N2E2_735 at 845.187 kb on + strand at 845.188 kb on - strand at 845.403 kb on + strand, within Pf6N2E2_736 at 845.403 kb on + strand, within Pf6N2E2_736 at 845.403 kb on + strand, within Pf6N2E2_736 at 845.403 kb on + strand, within Pf6N2E2_736 at 845.404 kb on - strand, within Pf6N2E2_736 at 845.404 kb on - strand, within Pf6N2E2_736 at 845.533 kb on + strand at 845.931 kb on + strand, within Pf6N2E2_737 at 845.932 kb on - strand, within Pf6N2E2_737 at 846.145 kb on + strand at 846.146 kb on - strand at 846.243 kb on + strand at 846.243 kb on + strand at 846.244 kb on - strand at 846.244 kb on - strand at 846.244 kb on - strand at 846.265 kb on + strand at 846.265 kb on + strand at 846.266 kb on - strand at 846.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 842,517 - Pf6N2E2_734 0.54 +0.7 842,684 + Pf6N2E2_734 0.70 +0.1 842,684 + Pf6N2E2_734 0.70 +0.7 842,684 + Pf6N2E2_734 0.70 +0.2 842,685 - Pf6N2E2_734 0.70 +0.5 842,975 + +1.3 842,981 + +0.3 842,981 + -0.5 842,981 + -2.0 842,981 + +0.9 843,088 + -0.5 843,088 + +0.2 843,088 + -1.0 843,088 + -0.2 843,089 - -0.9 843,128 - +0.2 843,128 - -0.2 843,233 - -2.1 843,234 + -1.3 843,234 + -0.1 843,235 - -3.0 843,235 - -1.9 843,235 - -1.9 843,238 + -0.9 843,238 + -1.3 843,238 + -1.6 843,238 + +1.1 843,239 - -2.1 843,239 - -1.0 843,239 - -1.9 843,239 - -3.1 843,239 - -1.1 843,239 - -2.9 843,285 - -2.8 843,519 + Pf6N2E2_735 0.13 -4.2 843,519 + Pf6N2E2_735 0.13 -2.1 843,519 + Pf6N2E2_735 0.13 -3.0 843,520 - Pf6N2E2_735 0.13 -2.1 843,520 - Pf6N2E2_735 0.13 -1.6 843,648 + Pf6N2E2_735 0.19 -0.8 843,676 + Pf6N2E2_735 0.21 +0.7 843,699 + Pf6N2E2_735 0.22 -2.1 843,699 + Pf6N2E2_735 0.22 -2.9 843,699 + Pf6N2E2_735 0.22 -3.1 843,700 - Pf6N2E2_735 0.22 -4.0 843,717 + Pf6N2E2_735 0.23 -2.1 843,717 + Pf6N2E2_735 0.23 -2.1 843,718 - Pf6N2E2_735 0.23 -0.9 843,718 - Pf6N2E2_735 0.23 -2.7 843,762 - Pf6N2E2_735 0.25 -2.1 843,967 - Pf6N2E2_735 0.35 -2.3 843,967 - Pf6N2E2_735 0.35 -1.5 843,967 - Pf6N2E2_735 0.35 -2.4 843,967 - Pf6N2E2_735 0.35 -4.2 843,967 - Pf6N2E2_735 0.35 -0.9 843,978 + Pf6N2E2_735 0.36 -3.5 843,978 + Pf6N2E2_735 0.36 -1.4 843,978 + Pf6N2E2_735 0.36 -2.6 843,978 + Pf6N2E2_735 0.36 -3.2 843,979 - Pf6N2E2_735 0.36 -1.3 843,979 - Pf6N2E2_735 0.36 -2.5 843,979 - Pf6N2E2_735 0.36 -3.9 843,979 - Pf6N2E2_735 0.36 -2.6 843,979 - Pf6N2E2_735 0.36 -1.3 843,979 - -1.9 843,979 - Pf6N2E2_735 0.36 -2.5 843,979 - Pf6N2E2_735 0.36 -1.4 844,039 - Pf6N2E2_735 0.39 -4.9 844,039 - Pf6N2E2_735 0.39 +0.7 844,039 - Pf6N2E2_735 0.39 -2.6 844,039 - Pf6N2E2_735 0.39 -0.6 844,039 - Pf6N2E2_735 0.39 -2.7 844,051 + Pf6N2E2_735 0.39 -2.3 844,168 - Pf6N2E2_735 0.45 -2.1 844,209 + Pf6N2E2_735 0.47 -1.3 844,210 - Pf6N2E2_735 0.47 -4.1 844,210 - Pf6N2E2_735 0.47 -3.5 844,210 - Pf6N2E2_735 0.47 -1.6 844,210 - Pf6N2E2_735 0.47 -2.9 844,272 + Pf6N2E2_735 0.50 -0.3 844,273 - Pf6N2E2_735 0.50 -1.4 844,273 - Pf6N2E2_735 0.50 -3.6 844,273 - Pf6N2E2_735 0.50 -1.6 844,530 + Pf6N2E2_735 0.63 +0.7 844,530 + Pf6N2E2_735 0.63 -0.3 844,531 - Pf6N2E2_735 0.63 -2.0 844,531 - Pf6N2E2_735 0.63 -0.9 844,531 - Pf6N2E2_735 0.63 -2.7 844,645 - Pf6N2E2_735 0.69 -2.4 844,650 + Pf6N2E2_735 0.69 -3.7 844,845 + Pf6N2E2_735 0.79 -2.3 844,846 - Pf6N2E2_735 0.79 -2.1 844,846 - Pf6N2E2_735 0.79 -1.6 844,846 - Pf6N2E2_735 0.79 -2.6 844,950 + Pf6N2E2_735 0.84 -2.6 844,950 + Pf6N2E2_735 0.84 -2.7 844,950 + Pf6N2E2_735 0.84 -0.6 844,950 + Pf6N2E2_735 0.84 -2.3 844,950 + Pf6N2E2_735 0.84 -1.6 844,951 - Pf6N2E2_735 0.84 -1.5 844,953 + Pf6N2E2_735 0.84 -2.8 844,954 - Pf6N2E2_735 0.84 -0.8 844,954 - Pf6N2E2_735 0.84 -2.9 845,014 + Pf6N2E2_735 0.87 -1.7 845,014 + Pf6N2E2_735 0.87 -1.0 845,015 - Pf6N2E2_735 0.87 -3.7 845,037 + Pf6N2E2_735 0.88 -3.4 845,037 + Pf6N2E2_735 0.88 -0.1 845,038 - Pf6N2E2_735 0.88 -3.0 845,038 - Pf6N2E2_735 0.88 -1.4 845,064 + Pf6N2E2_735 0.90 -2.7 845,064 + Pf6N2E2_735 0.90 -1.6 845,064 + Pf6N2E2_735 0.90 -2.9 845,064 + Pf6N2E2_735 0.90 -4.2 845,064 + Pf6N2E2_735 0.90 -1.7 845,065 - Pf6N2E2_735 0.90 -2.2 845,065 - Pf6N2E2_735 0.90 -2.7 845,065 - Pf6N2E2_735 0.90 -1.0 845,065 - Pf6N2E2_735 0.90 -3.5 845,187 + -2.5 845,188 - -2.8 845,403 + Pf6N2E2_736 0.62 -1.7 845,403 + Pf6N2E2_736 0.62 -1.4 845,403 + Pf6N2E2_736 0.62 -2.3 845,403 + Pf6N2E2_736 0.62 -2.3 845,404 - Pf6N2E2_736 0.63 -1.6 845,404 - Pf6N2E2_736 0.63 -1.5 845,533 + -2.4 845,931 + Pf6N2E2_737 0.67 -2.9 845,932 - Pf6N2E2_737 0.67 -3.2 846,145 + -1.7 846,146 - -1.9 846,243 + +1.1 846,243 + +0.4 846,244 - +0.4 846,244 - -0.5 846,244 - -0.6 846,265 + -0.4 846,265 + +0.6 846,266 - -0.3 846,266 - +0.1
Or see this region's nucleotide sequence