Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_728

Experiment: N1B4wt with N2E2m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_727 and Pf6N2E2_728 overlap by 23 nucleotidesPf6N2E2_728 and Pf6N2E2_729 are separated by 64 nucleotides Pf6N2E2_727 - Nitrate/nitrite transporter, at 831,495 to 832,796 _727 Pf6N2E2_728 - Nitrate/nitrite transporter, at 832,774 to 834,171 _728 Pf6N2E2_729 - Respiratory nitrate reductase alpha chain (EC 1.7.99.4), at 834,236 to 838,009 _729 Position (kb) 832 833 834 835Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 831.852 kb on + strand, within Pf6N2E2_727at 831.852 kb on + strand, within Pf6N2E2_727at 831.852 kb on + strand, within Pf6N2E2_727at 832.134 kb on + strand, within Pf6N2E2_727at 832.135 kb on - strand, within Pf6N2E2_727at 832.135 kb on - strand, within Pf6N2E2_727at 832.137 kb on + strand, within Pf6N2E2_727at 832.159 kb on - strand, within Pf6N2E2_727at 832.159 kb on - strand, within Pf6N2E2_727at 832.188 kb on + strand, within Pf6N2E2_727at 832.188 kb on + strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.189 kb on - strand, within Pf6N2E2_727at 832.191 kb on + strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.192 kb on - strand, within Pf6N2E2_727at 832.503 kb on + strand, within Pf6N2E2_727at 832.503 kb on + strand, within Pf6N2E2_727at 832.504 kb on - strand, within Pf6N2E2_727at 832.504 kb on - strand, within Pf6N2E2_727at 832.650 kb on + strand, within Pf6N2E2_727at 832.650 kb on + strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.651 kb on - strand, within Pf6N2E2_727at 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.683 kb on + strandat 832.684 kb on - strandat 832.684 kb on - strandat 832.684 kb on - strandat 833.042 kb on - strand, within Pf6N2E2_728at 833.239 kb on + strand, within Pf6N2E2_728at 833.375 kb on + strand, within Pf6N2E2_728at 833.812 kb on + strand, within Pf6N2E2_728at 833.813 kb on - strand, within Pf6N2E2_728at 834.036 kb on + strandat 834.036 kb on + strandat 834.037 kb on - strandat 834.088 kb on + strandat 834.088 kb on + strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.089 kb on - strandat 834.139 kb on + strandat 834.139 kb on + strandat 834.170 kb on - strandat 834.180 kb on + strandat 834.246 kb on + strandat 834.247 kb on - strandat 834.275 kb on - strandat 834.275 kb on - strandat 834.341 kb on + strandat 834.359 kb on + strandat 834.359 kb on + strandat 834.423 kb on - strandat 834.489 kb on + strandat 834.490 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.540 kb on - strandat 834.545 kb on + strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.546 kb on - strandat 834.678 kb on - strand, within Pf6N2E2_729at 834.678 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.680 kb on - strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.758 kb on + strand, within Pf6N2E2_729at 834.759 kb on - strand, within Pf6N2E2_729at 834.830 kb on + strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.885 kb on - strand, within Pf6N2E2_729at 834.965 kb on + strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.978 kb on - strand, within Pf6N2E2_729at 834.982 kb on + strand, within Pf6N2E2_729at 835.049 kb on + strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.050 kb on - strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.085 kb on + strand, within Pf6N2E2_729at 835.086 kb on - strand, within Pf6N2E2_729

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C
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831,852 + Pf6N2E2_727 0.27 -0.9
831,852 + Pf6N2E2_727 0.27 -0.3
831,852 + Pf6N2E2_727 0.27 +0.5
832,134 + Pf6N2E2_727 0.49 +0.3
832,135 - Pf6N2E2_727 0.49 +0.6
832,135 - Pf6N2E2_727 0.49 -1.0
832,137 + Pf6N2E2_727 0.49 -0.7
832,159 - Pf6N2E2_727 0.51 -0.8
832,159 - Pf6N2E2_727 0.51 +0.4
832,188 + Pf6N2E2_727 0.53 -0.3
832,188 + Pf6N2E2_727 0.53 +0.3
832,189 - Pf6N2E2_727 0.53 -0.4
832,189 - Pf6N2E2_727 0.53 -0.9
832,189 - Pf6N2E2_727 0.53 +0.4
832,189 - Pf6N2E2_727 0.53 -0.6
832,191 + Pf6N2E2_727 0.53 -1.3
832,192 - Pf6N2E2_727 0.54 -1.1
832,192 - Pf6N2E2_727 0.54 -1.1
832,192 - Pf6N2E2_727 0.54 -0.2
832,192 - Pf6N2E2_727 0.54 -0.5
832,192 - Pf6N2E2_727 0.54 -0.6
832,192 - Pf6N2E2_727 0.54 -0.8
832,503 + Pf6N2E2_727 0.77 -0.8
832,503 + Pf6N2E2_727 0.77 -0.2
832,504 - Pf6N2E2_727 0.77 -0.5
832,504 - Pf6N2E2_727 0.77 -0.8
832,650 + Pf6N2E2_727 0.89 -0.3
832,650 + Pf6N2E2_727 0.89 -0.3
832,651 - Pf6N2E2_727 0.89 -0.6
832,651 - Pf6N2E2_727 0.89 -0.6
832,651 - Pf6N2E2_727 0.89 -0.8
832,683 + +0.3
832,683 + -0.6
832,683 + +0.6
832,683 + +0.8
832,683 + -0.4
832,684 - -0.3
832,684 - -0.2
832,684 - -0.5
833,042 - Pf6N2E2_728 0.19 -2.7
833,239 + Pf6N2E2_728 0.33 +1.3
833,375 + Pf6N2E2_728 0.43 +1.2
833,812 + Pf6N2E2_728 0.74 +0.5
833,813 - Pf6N2E2_728 0.74 -1.1
834,036 + +0.7
834,036 + +0.6
834,037 - +0.4
834,088 + +0.2
834,088 + +0.7
834,089 - +0.0
834,089 - -2.3
834,089 - -2.4
834,089 - -2.5
834,089 - -1.2
834,089 - -2.3
834,139 + +1.7
834,139 + +0.3
834,170 - -1.6
834,180 + +0.8
834,246 + -1.0
834,247 - -3.0
834,275 - -1.5
834,275 - -2.1
834,341 + -1.3
834,359 + -0.3
834,359 + +0.0
834,423 - -1.3
834,489 + -2.0
834,490 - -2.0
834,540 - -1.0
834,540 - -1.1
834,540 - -1.8
834,540 - -1.0
834,545 + -0.3
834,546 - -0.6
834,546 - -1.0
834,546 - -1.0
834,546 - -1.5
834,546 - -1.2
834,678 - Pf6N2E2_729 0.12 -0.5
834,678 - Pf6N2E2_729 0.12 -1.6
834,680 - Pf6N2E2_729 0.12 -2.0
834,680 - Pf6N2E2_729 0.12 -1.1
834,680 - Pf6N2E2_729 0.12 -1.3
834,680 - Pf6N2E2_729 0.12 -0.3
834,680 - Pf6N2E2_729 0.12 +0.0
834,680 - Pf6N2E2_729 0.12 -1.4
834,758 + Pf6N2E2_729 0.14 -3.3
834,758 + Pf6N2E2_729 0.14 -1.2
834,759 - Pf6N2E2_729 0.14 -0.8
834,830 + Pf6N2E2_729 0.16 -1.2
834,885 - Pf6N2E2_729 0.17 -1.1
834,885 - Pf6N2E2_729 0.17 -0.6
834,885 - Pf6N2E2_729 0.17 -1.5
834,885 - Pf6N2E2_729 0.17 -1.0
834,885 - Pf6N2E2_729 0.17 -0.8
834,965 + Pf6N2E2_729 0.19 -2.3
834,978 - Pf6N2E2_729 0.20 -1.2
834,978 - Pf6N2E2_729 0.20 +0.6
834,982 + Pf6N2E2_729 0.20 -1.9
835,049 + Pf6N2E2_729 0.22 -0.2
835,050 - Pf6N2E2_729 0.22 -0.6
835,050 - Pf6N2E2_729 0.22 -0.1
835,050 - Pf6N2E2_729 0.22 -0.6
835,050 - Pf6N2E2_729 0.22 +2.3
835,085 + Pf6N2E2_729 0.22 -1.3
835,085 + Pf6N2E2_729 0.22 -1.8
835,086 - Pf6N2E2_729 0.23 -1.2

Or see this region's nucleotide sequence