Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_711

Experiment: N1B4wt with N2E2m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_710 and Pf6N2E2_711 are separated by 181 nucleotidesPf6N2E2_711 and Pf6N2E2_712 are separated by 4 nucleotidesPf6N2E2_712 and Pf6N2E2_713 overlap by 4 nucleotides Pf6N2E2_710 - Nitric-oxide reductase subunit B (EC 1.7.99.7), at 813,257 to 814,684 _710 Pf6N2E2_711 - Nitric oxide reductase activation protein NorD, at 814,866 to 816,704 _711 Pf6N2E2_712 - hypothetical protein, at 816,709 to 816,909 _712 Pf6N2E2_713 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 816,906 to 817,589 _713 Position (kb) 814 815 816 817Strain fitness (log2 ratio) -2 -1 0 1 2 3at 813.980 kb on + strand, within Pf6N2E2_710at 813.980 kb on + strand, within Pf6N2E2_710at 813.980 kb on + strand, within Pf6N2E2_710at 813.981 kb on - strand, within Pf6N2E2_710at 813.981 kb on - strand, within Pf6N2E2_710at 814.262 kb on + strand, within Pf6N2E2_710at 814.262 kb on + strand, within Pf6N2E2_710at 814.262 kb on + strand, within Pf6N2E2_710at 814.263 kb on - strand, within Pf6N2E2_710at 814.263 kb on - strand, within Pf6N2E2_710at 814.263 kb on - strand, within Pf6N2E2_710at 814.265 kb on + strand, within Pf6N2E2_710at 814.265 kb on + strand, within Pf6N2E2_710at 814.265 kb on + strand, within Pf6N2E2_710at 814.266 kb on - strand, within Pf6N2E2_710at 814.313 kb on + strand, within Pf6N2E2_710at 814.313 kb on + strand, within Pf6N2E2_710at 814.352 kb on + strand, within Pf6N2E2_710at 814.353 kb on - strand, within Pf6N2E2_710at 814.758 kb on + strandat 814.758 kb on + strandat 814.758 kb on + strandat 814.759 kb on - strandat 814.850 kb on + strandat 814.851 kb on - strandat 814.851 kb on - strandat 814.861 kb on + strandat 815.533 kb on - strand, within Pf6N2E2_711at 815.533 kb on - strand, within Pf6N2E2_711at 815.533 kb on - strand, within Pf6N2E2_711at 815.744 kb on + strand, within Pf6N2E2_711at 815.745 kb on - strand, within Pf6N2E2_711at 815.898 kb on - strand, within Pf6N2E2_711at 816.162 kb on - strand, within Pf6N2E2_711at 816.267 kb on + strand, within Pf6N2E2_711at 816.267 kb on + strand, within Pf6N2E2_711at 816.267 kb on + strand, within Pf6N2E2_711at 816.268 kb on - strand, within Pf6N2E2_711at 816.268 kb on - strand, within Pf6N2E2_711at 816.381 kb on + strand, within Pf6N2E2_711at 816.381 kb on + strand, within Pf6N2E2_711at 816.381 kb on + strand, within Pf6N2E2_711at 816.381 kb on + strand, within Pf6N2E2_711at 816.382 kb on - strand, within Pf6N2E2_711at 816.382 kb on - strand, within Pf6N2E2_711at 816.382 kb on - strand, within Pf6N2E2_711at 816.382 kb on - strand, within Pf6N2E2_711at 816.495 kb on + strand, within Pf6N2E2_711at 816.495 kb on + strand, within Pf6N2E2_711at 816.495 kb on + strand, within Pf6N2E2_711at 816.601 kb on - strandat 816.609 kb on + strandat 816.609 kb on + strandat 816.609 kb on + strandat 816.609 kb on + strandat 816.609 kb on + strandat 816.610 kb on - strandat 816.610 kb on - strandat 816.610 kb on - strandat 816.610 kb on - strandat 816.610 kb on - strandat 816.610 kb on - strandat 816.631 kb on - strandat 816.681 kb on + strandat 816.682 kb on - strandat 816.884 kb on + strand, within Pf6N2E2_712at 816.884 kb on + strand, within Pf6N2E2_712at 816.885 kb on - strand, within Pf6N2E2_712at 816.885 kb on - strand, within Pf6N2E2_712at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.243 kb on + strand, within Pf6N2E2_713at 817.244 kb on - strand, within Pf6N2E2_713at 817.244 kb on - strand, within Pf6N2E2_713at 817.244 kb on - strand, within Pf6N2E2_713at 817.244 kb on - strandat 817.244 kb on - strand, within Pf6N2E2_713at 817.244 kb on - strand, within Pf6N2E2_713at 817.247 kb on + strand, within Pf6N2E2_713at 817.264 kb on + strand, within Pf6N2E2_713at 817.266 kb on + strand, within Pf6N2E2_713at 817.266 kb on + strand, within Pf6N2E2_713at 817.267 kb on - strand, within Pf6N2E2_713at 817.267 kb on - strand, within Pf6N2E2_713at 817.303 kb on + strand, within Pf6N2E2_713at 817.304 kb on - strand, within Pf6N2E2_713at 817.429 kb on + strand, within Pf6N2E2_713at 817.605 kb on + strandat 817.606 kb on - strandat 817.606 kb on - strandat 817.606 kb on - strandat 817.606 kb on - strandat 817.606 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C
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813,980 + Pf6N2E2_710 0.51 -0.8
813,980 + Pf6N2E2_710 0.51 -0.9
813,980 + Pf6N2E2_710 0.51 -0.5
813,981 - Pf6N2E2_710 0.51 +3.3
813,981 - Pf6N2E2_710 0.51 -0.5
814,262 + Pf6N2E2_710 0.70 -0.3
814,262 + Pf6N2E2_710 0.70 -0.3
814,262 + Pf6N2E2_710 0.70 -0.5
814,263 - Pf6N2E2_710 0.70 -0.7
814,263 - Pf6N2E2_710 0.70 -0.3
814,263 - Pf6N2E2_710 0.70 -1.0
814,265 + Pf6N2E2_710 0.71 -0.7
814,265 + Pf6N2E2_710 0.71 -1.3
814,265 + Pf6N2E2_710 0.71 -1.0
814,266 - Pf6N2E2_710 0.71 -1.6
814,313 + Pf6N2E2_710 0.74 -1.3
814,313 + Pf6N2E2_710 0.74 +0.8
814,352 + Pf6N2E2_710 0.77 -0.4
814,353 - Pf6N2E2_710 0.77 -0.7
814,758 + +0.1
814,758 + -0.2
814,758 + -0.1
814,759 - +0.5
814,850 + +0.0
814,851 - -0.1
814,851 - -0.4
814,861 + +0.0
815,533 - Pf6N2E2_711 0.36 +0.1
815,533 - Pf6N2E2_711 0.36 -0.3
815,533 - Pf6N2E2_711 0.36 +0.7
815,744 + Pf6N2E2_711 0.48 +0.0
815,745 - Pf6N2E2_711 0.48 -0.7
815,898 - Pf6N2E2_711 0.56 -1.6
816,162 - Pf6N2E2_711 0.70 -0.4
816,267 + Pf6N2E2_711 0.76 -0.1
816,267 + Pf6N2E2_711 0.76 -0.1
816,267 + Pf6N2E2_711 0.76 +0.3
816,268 - Pf6N2E2_711 0.76 +0.7
816,268 - Pf6N2E2_711 0.76 -0.8
816,381 + Pf6N2E2_711 0.82 -0.0
816,381 + Pf6N2E2_711 0.82 -1.7
816,381 + Pf6N2E2_711 0.82 +0.1
816,381 + Pf6N2E2_711 0.82 -0.9
816,382 - Pf6N2E2_711 0.82 -0.2
816,382 - Pf6N2E2_711 0.82 -0.9
816,382 - Pf6N2E2_711 0.82 -1.3
816,382 - Pf6N2E2_711 0.82 -2.3
816,495 + Pf6N2E2_711 0.89 -0.3
816,495 + Pf6N2E2_711 0.89 -0.3
816,495 + Pf6N2E2_711 0.89 -1.1
816,601 - -1.8
816,609 + -0.5
816,609 + -0.5
816,609 + +0.1
816,609 + -0.3
816,609 + -0.2
816,610 - +0.2
816,610 - -0.7
816,610 - -0.3
816,610 - -1.0
816,610 - -0.4
816,610 - +0.5
816,631 - -1.0
816,681 + -0.3
816,682 - +0.0
816,884 + Pf6N2E2_712 0.87 -0.7
816,884 + Pf6N2E2_712 0.87 -0.7
816,885 - Pf6N2E2_712 0.88 +0.2
816,885 - Pf6N2E2_712 0.88 +0.1
817,243 + Pf6N2E2_713 0.49 -0.8
817,243 + Pf6N2E2_713 0.49 +0.6
817,243 + Pf6N2E2_713 0.49 -0.6
817,243 + Pf6N2E2_713 0.49 -2.3
817,243 + Pf6N2E2_713 0.49 +0.0
817,243 + Pf6N2E2_713 0.49 -2.3
817,243 + Pf6N2E2_713 0.49 -0.9
817,244 - Pf6N2E2_713 0.49 -0.2
817,244 - Pf6N2E2_713 0.49 -1.0
817,244 - Pf6N2E2_713 0.49 -0.2
817,244 - +0.7
817,244 - Pf6N2E2_713 0.49 +0.3
817,244 - Pf6N2E2_713 0.49 +0.4
817,247 + Pf6N2E2_713 0.50 +0.2
817,264 + Pf6N2E2_713 0.52 +0.7
817,266 + Pf6N2E2_713 0.53 -1.3
817,266 + Pf6N2E2_713 0.53 -0.6
817,267 - Pf6N2E2_713 0.53 -0.7
817,267 - Pf6N2E2_713 0.53 -0.3
817,303 + Pf6N2E2_713 0.58 +0.2
817,304 - Pf6N2E2_713 0.58 -1.1
817,429 + Pf6N2E2_713 0.76 -0.5
817,605 + -0.4
817,606 - +0.2
817,606 - +1.0
817,606 - -0.0
817,606 - -0.9
817,606 - +1.1

Or see this region's nucleotide sequence