Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_702 and Pf6N2E2_703 overlap by 4 nucleotides Pf6N2E2_703 and Pf6N2E2_704 are separated by 190 nucleotides Pf6N2E2_704 and Pf6N2E2_705 are separated by 24 nucleotides
Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193
_702
Pf6N2E2_703 - Cytochrome c55X precursor NirC, at 808,190 to 808,519
_703
Pf6N2E2_704 - Cytochrome c551/c552, at 808,710 to 809,018
_704
Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722
_705
Position (kb) 808
809
810 Strain fitness (log2 ratio) -2
-1
0
1 at 807.814 kb on - strand, within Pf6N2E2_702 at 807.835 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.534 kb on + strand at 808.534 kb on + strand at 808.535 kb on - strand at 808.711 kb on + strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.715 kb on + strand at 808.716 kb on - strand at 808.716 kb on - strand at 808.805 kb on + strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.901 kb on + strand, within Pf6N2E2_704 at 808.902 kb on - strand, within Pf6N2E2_704 at 809.010 kb on - strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.048 kb on - strand at 809.048 kb on - strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.069 kb on - strand at 809.218 kb on + strand, within Pf6N2E2_705 at 809.219 kb on - strand, within Pf6N2E2_705 at 809.263 kb on + strand, within Pf6N2E2_705 at 809.264 kb on - strand, within Pf6N2E2_705 at 809.368 kb on + strand, within Pf6N2E2_705 at 809.368 kb on + strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.453 kb on - strand, within Pf6N2E2_705 at 809.453 kb on - strand, within Pf6N2E2_705 at 809.666 kb on - strand, within Pf6N2E2_705 at 809.666 kb on - strand, within Pf6N2E2_705 at 809.749 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.960 kb on - strand, within Pf6N2E2_705
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 807,814 - Pf6N2E2_702 0.68 -0.6 807,835 + Pf6N2E2_702 0.70 -1.6 807,970 + Pf6N2E2_702 0.81 -1.2 807,970 + Pf6N2E2_702 0.81 +0.1 807,970 + Pf6N2E2_702 0.81 -0.5 807,970 + Pf6N2E2_702 0.81 -0.8 807,970 + Pf6N2E2_702 0.81 -1.0 807,971 - Pf6N2E2_702 0.81 -0.8 807,971 - Pf6N2E2_702 0.81 +0.2 807,971 - Pf6N2E2_702 0.81 -0.5 807,971 - Pf6N2E2_702 0.81 -0.8 808,377 + Pf6N2E2_703 0.57 -0.8 808,377 + Pf6N2E2_703 0.57 -1.0 808,377 + Pf6N2E2_703 0.57 -0.9 808,377 + Pf6N2E2_703 0.57 -0.5 808,377 + Pf6N2E2_703 0.57 -0.8 808,377 + Pf6N2E2_703 0.57 +0.7 808,378 - Pf6N2E2_703 0.57 -2.1 808,378 - Pf6N2E2_703 0.57 -0.5 808,534 + -2.3 808,534 + -0.5 808,535 - +1.3 808,711 + -0.3 808,712 - -0.3 808,712 - -0.7 808,712 - -0.9 808,715 + -1.3 808,716 - -1.5 808,716 - +0.7 808,805 + Pf6N2E2_704 0.31 -1.9 808,806 - Pf6N2E2_704 0.31 -0.4 808,806 - Pf6N2E2_704 0.31 -0.6 808,901 + Pf6N2E2_704 0.62 -0.4 808,902 - Pf6N2E2_704 0.62 -0.8 809,010 - -2.3 809,047 + +0.7 809,047 + +1.5 809,047 + -0.0 809,047 + -2.6 809,047 + -0.7 809,048 - +0.4 809,048 - -1.4 809,068 + -1.4 809,068 + -0.8 809,068 + -0.1 809,068 + -0.7 809,068 + +0.7 809,068 + -1.3 809,068 + -1.6 809,069 - -0.5 809,218 + Pf6N2E2_705 0.10 +0.1 809,219 - Pf6N2E2_705 0.10 +0.2 809,263 + Pf6N2E2_705 0.13 -0.9 809,264 - Pf6N2E2_705 0.13 -1.5 809,368 + Pf6N2E2_705 0.19 -1.0 809,368 + Pf6N2E2_705 0.19 -2.2 809,369 - Pf6N2E2_705 0.19 -2.1 809,369 - Pf6N2E2_705 0.19 -1.1 809,369 - Pf6N2E2_705 0.19 -1.1 809,369 - Pf6N2E2_705 0.19 -2.5 809,369 - Pf6N2E2_705 0.19 -1.9 809,453 - Pf6N2E2_705 0.24 -0.5 809,453 - Pf6N2E2_705 0.24 -1.4 809,666 - Pf6N2E2_705 0.37 -1.1 809,666 - Pf6N2E2_705 0.37 -0.6 809,749 + Pf6N2E2_705 0.42 -0.3 809,959 + Pf6N2E2_705 0.55 -1.4 809,959 + Pf6N2E2_705 0.55 -1.0 809,959 + Pf6N2E2_705 0.55 -0.6 809,959 + Pf6N2E2_705 0.55 +0.2 809,960 - Pf6N2E2_705 0.55 -1.1
Or see this region's nucleotide sequence