Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_701

Experiment: N1B4wt with N2E2m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotidesPf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotidesPf6N2E2_700 and Pf6N2E2_701 overlap by 4 nucleotidesPf6N2E2_701 and Pf6N2E2_702 are separated by 2 nucleotides Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634 _698 Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052 _699 Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557 _700 Pf6N2E2_701 - Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL, at 806,554 to 807,012 _701 Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193 _702 Position (kb) 806 807 808Strain fitness (log2 ratio) -3 -2 -1 0 1at 805.623 kb on - strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.637 kb on + strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.638 kb on - strandat 805.769 kb on + strand, within Pf6N2E2_699at 805.769 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.906 kb on + strand, within Pf6N2E2_699at 805.907 kb on - strand, within Pf6N2E2_699at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.223 kb on + strand, within Pf6N2E2_700at 806.224 kb on - strand, within Pf6N2E2_700at 806.257 kb on - strand, within Pf6N2E2_700at 806.708 kb on + strand, within Pf6N2E2_701at 806.708 kb on + strand, within Pf6N2E2_701at 806.709 kb on - strand, within Pf6N2E2_701at 806.726 kb on + strand, within Pf6N2E2_701at 806.726 kb on + strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 806.727 kb on - strand, within Pf6N2E2_701at 807.451 kb on + strand, within Pf6N2E2_702at 807.452 kb on - strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.568 kb on + strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.569 kb on - strand, within Pf6N2E2_702at 807.572 kb on - strand, within Pf6N2E2_702at 807.572 kb on - strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.601 kb on + strand, within Pf6N2E2_702at 807.602 kb on - strand, within Pf6N2E2_702at 807.602 kb on - strand, within Pf6N2E2_702at 807.814 kb on - strand, within Pf6N2E2_702at 807.835 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.970 kb on + strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702at 807.971 kb on - strand, within Pf6N2E2_702

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C
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805,623 - +1.2
805,637 + +0.0
805,637 + +0.2
805,637 + -0.3
805,637 + -1.1
805,637 + -1.3
805,637 + -1.3
805,637 + -0.3
805,637 + -0.3
805,638 - +0.1
805,638 - -1.6
805,638 - -1.1
805,638 - -2.1
805,638 - +0.7
805,638 - -1.0
805,638 - -1.0
805,638 - -0.3
805,638 - -1.5
805,638 - -0.6
805,769 + Pf6N2E2_699 0.36 +0.1
805,769 + Pf6N2E2_699 0.36 -0.8
805,906 + Pf6N2E2_699 0.67 -1.0
805,906 + Pf6N2E2_699 0.67 -0.2
805,907 - Pf6N2E2_699 0.67 +0.3
806,223 + Pf6N2E2_700 0.35 -1.8
806,223 + Pf6N2E2_700 0.35 -0.7
806,223 + Pf6N2E2_700 0.35 -0.7
806,223 + Pf6N2E2_700 0.35 -1.6
806,223 + Pf6N2E2_700 0.35 -0.3
806,223 + Pf6N2E2_700 0.35 -0.4
806,224 - Pf6N2E2_700 0.35 -1.4
806,257 - Pf6N2E2_700 0.41 -0.1
806,708 + Pf6N2E2_701 0.34 +0.6
806,708 + Pf6N2E2_701 0.34 -1.2
806,709 - Pf6N2E2_701 0.34 -0.4
806,726 + Pf6N2E2_701 0.37 -0.8
806,726 + Pf6N2E2_701 0.37 +0.4
806,727 - Pf6N2E2_701 0.38 -1.1
806,727 - Pf6N2E2_701 0.38 -1.3
806,727 - Pf6N2E2_701 0.38 -1.0
806,727 - Pf6N2E2_701 0.38 -1.7
807,451 + Pf6N2E2_702 0.37 -3.2
807,452 - Pf6N2E2_702 0.37 -1.2
807,568 + Pf6N2E2_702 0.47 -1.6
807,568 + Pf6N2E2_702 0.47 +0.5
807,568 + Pf6N2E2_702 0.47 -2.1
807,568 + Pf6N2E2_702 0.47 -2.1
807,568 + Pf6N2E2_702 0.47 -1.5
807,568 + Pf6N2E2_702 0.47 -0.7
807,568 + Pf6N2E2_702 0.47 -1.3
807,568 + Pf6N2E2_702 0.47 -0.9
807,568 + Pf6N2E2_702 0.47 -0.4
807,568 + Pf6N2E2_702 0.47 -0.6
807,569 - Pf6N2E2_702 0.47 -0.7
807,569 - Pf6N2E2_702 0.47 -1.2
807,569 - Pf6N2E2_702 0.47 -0.6
807,569 - Pf6N2E2_702 0.47 -1.4
807,569 - Pf6N2E2_702 0.47 -1.0
807,572 - Pf6N2E2_702 0.47 +0.7
807,572 - Pf6N2E2_702 0.47 -0.3
807,601 + Pf6N2E2_702 0.50 -1.4
807,601 + Pf6N2E2_702 0.50 +0.7
807,601 + Pf6N2E2_702 0.50 -1.1
807,601 + Pf6N2E2_702 0.50 -1.2
807,601 + Pf6N2E2_702 0.50 +1.5
807,602 - Pf6N2E2_702 0.50 -1.1
807,602 - Pf6N2E2_702 0.50 -1.3
807,814 - Pf6N2E2_702 0.68 -0.6
807,835 + Pf6N2E2_702 0.70 -1.6
807,970 + Pf6N2E2_702 0.81 -1.2
807,970 + Pf6N2E2_702 0.81 +0.1
807,970 + Pf6N2E2_702 0.81 -0.5
807,970 + Pf6N2E2_702 0.81 -0.8
807,970 + Pf6N2E2_702 0.81 -1.0
807,971 - Pf6N2E2_702 0.81 -0.8
807,971 - Pf6N2E2_702 0.81 +0.2
807,971 - Pf6N2E2_702 0.81 -0.5
807,971 - Pf6N2E2_702 0.81 -0.8

Or see this region's nucleotide sequence