Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_695 and Pf6N2E2_696 overlap by 4 nucleotides Pf6N2E2_696 and Pf6N2E2_697 are separated by 40 nucleotides Pf6N2E2_697 and Pf6N2E2_698 are separated by 28 nucleotides Pf6N2E2_698 and Pf6N2E2_699 overlap by 26 nucleotides Pf6N2E2_699 and Pf6N2E2_700 overlap by 8 nucleotides
Pf6N2E2_695 - Nitrite reductase associated c-type cytochorome NirN, at 801,515 to 803,011
_695
Pf6N2E2_696 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 803,008 to 803,850
_696
Pf6N2E2_697 - Heme d1 biosynthesis protein NirJ, at 803,891 to 805,069
_697
Pf6N2E2_698 - Heme d1 biosynthesis protein NirH, at 805,098 to 805,634
_698
Pf6N2E2_699 - Heme d1 biosynthesis protein NirG, at 805,609 to 806,052
_699
Pf6N2E2_700 - Heme d1 biosynthesis protein NirL, at 806,045 to 806,557
_700
Position (kb) 803
804
805
806 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.917 kb on + strand at 802.918 kb on - strand at 802.918 kb on - strand at 802.982 kb on + strand at 802.982 kb on + strand at 802.983 kb on - strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.985 kb on + strand at 802.986 kb on - strand at 802.986 kb on - strand at 802.986 kb on - strand at 803.232 kb on + strand, within Pf6N2E2_696 at 803.232 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.297 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.429 kb on + strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.430 kb on - strand, within Pf6N2E2_696 at 803.499 kb on - strand, within Pf6N2E2_696 at 803.646 kb on - strand, within Pf6N2E2_696 at 803.705 kb on + strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 803.706 kb on - strand, within Pf6N2E2_696 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.165 kb on + strand, within Pf6N2E2_697 at 804.191 kb on - strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.456 kb on + strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.457 kb on - strand, within Pf6N2E2_697 at 804.486 kb on - strand, within Pf6N2E2_697 at 804.493 kb on - strand, within Pf6N2E2_697 at 804.681 kb on + strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.682 kb on - strand, within Pf6N2E2_697 at 804.777 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.930 kb on + strand, within Pf6N2E2_697 at 804.931 kb on - strand, within Pf6N2E2_697 at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.070 kb on - strand at 805.210 kb on + strand, within Pf6N2E2_698 at 805.211 kb on - strand, within Pf6N2E2_698 at 805.623 kb on - strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.637 kb on + strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.638 kb on - strand at 805.769 kb on + strand, within Pf6N2E2_699 at 805.769 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.906 kb on + strand, within Pf6N2E2_699 at 805.907 kb on - strand, within Pf6N2E2_699
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 802,917 + +0.4 802,917 + +0.2 802,917 + +0.1 802,917 + +0.3 802,918 - -1.1 802,918 - -0.3 802,982 + -0.1 802,982 + -2.0 802,983 - -1.2 802,985 + +0.2 802,985 + +0.0 802,985 + -0.7 802,986 - -0.3 802,986 - -0.7 802,986 - -0.4 803,232 + Pf6N2E2_696 0.27 +0.7 803,232 + Pf6N2E2_696 0.27 -0.8 803,297 + Pf6N2E2_696 0.34 -0.1 803,297 + Pf6N2E2_696 0.34 -1.5 803,297 + Pf6N2E2_696 0.34 +0.3 803,297 + Pf6N2E2_696 0.34 -0.8 803,429 + Pf6N2E2_696 0.50 +0.0 803,429 + Pf6N2E2_696 0.50 -0.0 803,430 - Pf6N2E2_696 0.50 -0.3 803,430 - Pf6N2E2_696 0.50 -0.8 803,499 - Pf6N2E2_696 0.58 -0.6 803,646 - Pf6N2E2_696 0.76 +0.7 803,705 + Pf6N2E2_696 0.83 -1.1 803,706 - Pf6N2E2_696 0.83 -0.5 803,706 - Pf6N2E2_696 0.83 -0.2 803,706 - Pf6N2E2_696 0.83 -0.7 804,165 + Pf6N2E2_697 0.23 -1.3 804,165 + Pf6N2E2_697 0.23 -1.4 804,165 + Pf6N2E2_697 0.23 -0.6 804,165 + Pf6N2E2_697 0.23 -0.4 804,191 - Pf6N2E2_697 0.25 -1.1 804,456 + Pf6N2E2_697 0.48 -1.1 804,456 + Pf6N2E2_697 0.48 -1.1 804,456 + Pf6N2E2_697 0.48 -0.5 804,457 - Pf6N2E2_697 0.48 -1.4 804,457 - Pf6N2E2_697 0.48 -0.3 804,457 - Pf6N2E2_697 0.48 -2.9 804,457 - Pf6N2E2_697 0.48 -1.4 804,486 - Pf6N2E2_697 0.50 -1.3 804,493 - Pf6N2E2_697 0.51 -1.7 804,681 + Pf6N2E2_697 0.67 -0.8 804,682 - Pf6N2E2_697 0.67 -1.0 804,682 - Pf6N2E2_697 0.67 -0.5 804,777 + Pf6N2E2_697 0.75 -0.5 804,930 + Pf6N2E2_697 0.88 -0.5 804,930 + Pf6N2E2_697 0.88 -0.7 804,930 + Pf6N2E2_697 0.88 +0.7 804,930 + Pf6N2E2_697 0.88 -0.3 804,930 + Pf6N2E2_697 0.88 -1.1 804,930 + Pf6N2E2_697 0.88 -0.7 804,931 - Pf6N2E2_697 0.88 -0.1 805,070 - -1.1 805,070 - +0.4 805,070 - +1.1 805,070 - +0.0 805,210 + Pf6N2E2_698 0.21 -0.5 805,211 - Pf6N2E2_698 0.21 -1.1 805,623 - +1.2 805,637 + +0.0 805,637 + +0.2 805,637 + -0.3 805,637 + -1.1 805,637 + -1.3 805,637 + -1.3 805,637 + -0.3 805,637 + -0.3 805,638 - +0.1 805,638 - -1.6 805,638 - -1.1 805,638 - -2.1 805,638 - +0.7 805,638 - -1.0 805,638 - -1.0 805,638 - -0.3 805,638 - -1.5 805,638 - -0.6 805,769 + Pf6N2E2_699 0.36 +0.1 805,769 + Pf6N2E2_699 0.36 -0.8 805,906 + Pf6N2E2_699 0.67 -1.0 805,906 + Pf6N2E2_699 0.67 -0.2 805,907 - Pf6N2E2_699 0.67 +0.3
Or see this region's nucleotide sequence