Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_6071

Experiment: N1B4wt with N2E2m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_6068 and Pf6N2E2_6069 are separated by 35 nucleotidesPf6N2E2_6069 and Pf6N2E2_6070 overlap by 4 nucleotidesPf6N2E2_6070 and Pf6N2E2_6071 overlap by 4 nucleotidesPf6N2E2_6071 and Pf6N2E2_6072 overlap by 4 nucleotides Pf6N2E2_6068 - FIG014801: Cation ABC transporter, periplasmic cation-binding protein, at 6,889,798 to 6,890,727 _6068 Pf6N2E2_6069 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 6,890,763 to 6,891,338 _6069 Pf6N2E2_6070 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 6,891,335 to 6,891,661 _6070 Pf6N2E2_6071 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 6,891,658 to 6,892,200 _6071 Pf6N2E2_6072 - FIG137594: Putative iron-regulated membrane protein, at 6,892,197 to 6,893,411 _6072 Position (kb) 6891 6892 6893Strain fitness (log2 ratio) -2 -1 0 1at 6890.721 kb on + strandat 6890.721 kb on + strandat 6890.721 kb on + strandat 6890.722 kb on - strandat 6890.972 kb on - strand, within Pf6N2E2_6069at 6890.972 kb on - strand, within Pf6N2E2_6069at 6891.139 kb on + strand, within Pf6N2E2_6069at 6891.139 kb on + strand, within Pf6N2E2_6069at 6891.140 kb on - strand, within Pf6N2E2_6069at 6891.280 kb on + strand, within Pf6N2E2_6069at 6891.280 kb on + strand, within Pf6N2E2_6069at 6891.280 kb on + strand, within Pf6N2E2_6069at 6891.280 kb on + strand, within Pf6N2E2_6069at 6891.280 kb on + strand, within Pf6N2E2_6069at 6891.281 kb on - strandat 6891.281 kb on - strandat 6891.402 kb on + strand, within Pf6N2E2_6070at 6891.402 kb on + strand, within Pf6N2E2_6070at 6891.403 kb on - strand, within Pf6N2E2_6070at 6891.403 kb on - strand, within Pf6N2E2_6070at 6891.403 kb on - strand, within Pf6N2E2_6070at 6891.888 kb on - strand, within Pf6N2E2_6071at 6891.888 kb on - strand, within Pf6N2E2_6071at 6891.888 kb on - strand, within Pf6N2E2_6071at 6891.910 kb on - strand, within Pf6N2E2_6071at 6892.085 kb on + strand, within Pf6N2E2_6071at 6892.085 kb on + strand, within Pf6N2E2_6071at 6892.086 kb on - strand, within Pf6N2E2_6071at 6892.498 kb on + strand, within Pf6N2E2_6072at 6892.522 kb on + strand, within Pf6N2E2_6072at 6892.522 kb on + strand, within Pf6N2E2_6072at 6892.522 kb on + strand, within Pf6N2E2_6072at 6892.523 kb on - strand, within Pf6N2E2_6072at 6892.567 kb on + strand, within Pf6N2E2_6072at 6892.817 kb on + strand, within Pf6N2E2_6072at 6892.818 kb on - strand, within Pf6N2E2_6072at 6892.909 kb on + strand, within Pf6N2E2_6072at 6892.909 kb on + strand, within Pf6N2E2_6072at 6892.909 kb on + strand, within Pf6N2E2_6072at 6892.909 kb on + strand, within Pf6N2E2_6072at 6892.910 kb on - strand, within Pf6N2E2_6072at 6892.969 kb on + strand, within Pf6N2E2_6072at 6892.984 kb on + strand, within Pf6N2E2_6072at 6892.984 kb on + strand, within Pf6N2E2_6072at 6892.984 kb on + strand, within Pf6N2E2_6072at 6892.985 kb on - strand, within Pf6N2E2_6072at 6893.080 kb on + strand, within Pf6N2E2_6072at 6893.092 kb on + strand, within Pf6N2E2_6072at 6893.092 kb on + strand, within Pf6N2E2_6072at 6893.092 kb on + strand, within Pf6N2E2_6072at 6893.092 kb on + strand, within Pf6N2E2_6072at 6893.092 kb on + strand, within Pf6N2E2_6072

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C
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6,890,721 + +0.4
6,890,721 + -0.2
6,890,721 + +1.4
6,890,722 - +0.6
6,890,972 - Pf6N2E2_6069 0.36 -0.0
6,890,972 - Pf6N2E2_6069 0.36 -0.2
6,891,139 + Pf6N2E2_6069 0.65 -0.4
6,891,139 + Pf6N2E2_6069 0.65 +0.9
6,891,140 - Pf6N2E2_6069 0.65 -1.3
6,891,280 + Pf6N2E2_6069 0.90 +1.3
6,891,280 + Pf6N2E2_6069 0.90 -0.6
6,891,280 + Pf6N2E2_6069 0.90 +1.2
6,891,280 + Pf6N2E2_6069 0.90 +0.1
6,891,280 + Pf6N2E2_6069 0.90 -0.3
6,891,281 - -0.3
6,891,281 - +0.9
6,891,402 + Pf6N2E2_6070 0.20 +0.5
6,891,402 + Pf6N2E2_6070 0.20 +0.9
6,891,403 - Pf6N2E2_6070 0.21 +0.5
6,891,403 - Pf6N2E2_6070 0.21 +0.3
6,891,403 - Pf6N2E2_6070 0.21 -0.0
6,891,888 - Pf6N2E2_6071 0.42 -0.1
6,891,888 - Pf6N2E2_6071 0.42 -2.0
6,891,888 - Pf6N2E2_6071 0.42 -0.5
6,891,910 - Pf6N2E2_6071 0.46 +0.7
6,892,085 + Pf6N2E2_6071 0.79 -0.7
6,892,085 + Pf6N2E2_6071 0.79 -1.2
6,892,086 - Pf6N2E2_6071 0.79 -0.8
6,892,498 + Pf6N2E2_6072 0.25 -0.3
6,892,522 + Pf6N2E2_6072 0.27 -0.3
6,892,522 + Pf6N2E2_6072 0.27 -0.5
6,892,522 + Pf6N2E2_6072 0.27 +0.2
6,892,523 - Pf6N2E2_6072 0.27 -1.6
6,892,567 + Pf6N2E2_6072 0.30 -2.3
6,892,817 + Pf6N2E2_6072 0.51 -0.4
6,892,818 - Pf6N2E2_6072 0.51 -1.4
6,892,909 + Pf6N2E2_6072 0.59 +0.1
6,892,909 + Pf6N2E2_6072 0.59 +0.9
6,892,909 + Pf6N2E2_6072 0.59 -0.3
6,892,909 + Pf6N2E2_6072 0.59 -0.6
6,892,910 - Pf6N2E2_6072 0.59 -1.1
6,892,969 + Pf6N2E2_6072 0.64 -0.3
6,892,984 + Pf6N2E2_6072 0.65 +0.8
6,892,984 + Pf6N2E2_6072 0.65 +0.0
6,892,984 + Pf6N2E2_6072 0.65 -0.1
6,892,985 - Pf6N2E2_6072 0.65 -0.5
6,893,080 + Pf6N2E2_6072 0.73 +0.5
6,893,092 + Pf6N2E2_6072 0.74 +0.1
6,893,092 + Pf6N2E2_6072 0.74 -0.6
6,893,092 + Pf6N2E2_6072 0.74 +0.4
6,893,092 + Pf6N2E2_6072 0.74 -0.4
6,893,092 + Pf6N2E2_6072 0.74 -0.3

Or see this region's nucleotide sequence