Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4707 and Pf6N2E2_4708 are separated by 888 nucleotides Pf6N2E2_4708 and Pf6N2E2_4709 overlap by 42 nucleotides
Pf6N2E2_4707 - acetyltransferase, GNAT family, at 5,440,344 to 5,440,910
_4707
Pf6N2E2_4708 - hypothetical protein, at 5,441,799 to 5,442,002
_4708
Pf6N2E2_4709 - Cholesterol oxidase (EC 1.1.3.6), at 5,441,961 to 5,445,413
_4709
Position (kb) 5441
5442
5443 Strain fitness (log2 ratio) -2
-1
0
1 at 5440.812 kb on + strand, within Pf6N2E2_4707 at 5440.812 kb on + strand, within Pf6N2E2_4707 at 5440.813 kb on - strand, within Pf6N2E2_4707 at 5440.891 kb on - strand at 5440.891 kb on - strand at 5440.891 kb on - strand at 5440.963 kb on + strand at 5440.963 kb on + strand at 5440.963 kb on + strand at 5440.964 kb on - strand at 5440.964 kb on - strand at 5440.964 kb on - strand at 5440.964 kb on - strand at 5440.968 kb on - strand at 5441.126 kb on + strand at 5441.226 kb on - strand at 5441.226 kb on - strand at 5441.279 kb on - strand at 5441.392 kb on - strand at 5441.392 kb on - strand at 5441.428 kb on - strand at 5441.491 kb on + strand at 5441.491 kb on + strand at 5441.492 kb on - strand at 5441.498 kb on - strand at 5441.498 kb on - strand at 5441.498 kb on - strand at 5441.498 kb on - strand at 5441.498 kb on - strand at 5441.498 kb on - strand at 5441.599 kb on - strand at 5441.606 kb on + strand at 5441.607 kb on - strand at 5441.607 kb on - strand at 5441.614 kb on - strand at 5441.673 kb on - strand at 5441.673 kb on - strand at 5441.673 kb on - strand at 5441.726 kb on + strand at 5441.727 kb on - strand at 5441.727 kb on - strand at 5441.819 kb on + strand at 5441.819 kb on + strand at 5441.820 kb on - strand, within Pf6N2E2_4708 at 5441.820 kb on - strand, within Pf6N2E2_4708 at 5441.851 kb on - strand, within Pf6N2E2_4708 at 5442.003 kb on + strand at 5442.004 kb on - strand at 5442.487 kb on - strand, within Pf6N2E2_4709 at 5442.593 kb on + strand, within Pf6N2E2_4709 at 5442.594 kb on - strand, within Pf6N2E2_4709 at 5442.627 kb on + strand, within Pf6N2E2_4709 at 5442.627 kb on + strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.628 kb on - strand, within Pf6N2E2_4709 at 5442.727 kb on - strand, within Pf6N2E2_4709
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 5,440,812 + Pf6N2E2_4707 0.83 +0.7 5,440,812 + Pf6N2E2_4707 0.83 +0.6 5,440,813 - Pf6N2E2_4707 0.83 -0.1 5,440,891 - +0.9 5,440,891 - -1.2 5,440,891 - -0.2 5,440,963 + +0.3 5,440,963 + -0.5 5,440,963 + -0.1 5,440,964 - -0.3 5,440,964 - +0.1 5,440,964 - +0.7 5,440,964 - -1.1 5,440,968 - +0.6 5,441,126 + +0.3 5,441,226 - -0.3 5,441,226 - -0.4 5,441,279 - -0.6 5,441,392 - +1.4 5,441,392 - +0.0 5,441,428 - +0.2 5,441,491 + +0.7 5,441,491 + +0.5 5,441,492 - -1.8 5,441,498 - -0.4 5,441,498 - -0.3 5,441,498 - +0.6 5,441,498 - -1.3 5,441,498 - -0.1 5,441,498 - -0.9 5,441,599 - -0.0 5,441,606 + +1.5 5,441,607 - +0.2 5,441,607 - +0.7 5,441,614 - +0.6 5,441,673 - +1.2 5,441,673 - -0.6 5,441,673 - +0.0 5,441,726 + -0.2 5,441,727 - +0.5 5,441,727 - +0.2 5,441,819 + +0.0 5,441,819 + -0.4 5,441,820 - Pf6N2E2_4708 0.10 -1.3 5,441,820 - Pf6N2E2_4708 0.10 -0.1 5,441,851 - Pf6N2E2_4708 0.25 -1.3 5,442,003 + -0.4 5,442,004 - -0.5 5,442,487 - Pf6N2E2_4709 0.15 +0.1 5,442,593 + Pf6N2E2_4709 0.18 +0.7 5,442,594 - Pf6N2E2_4709 0.18 -0.5 5,442,627 + Pf6N2E2_4709 0.19 -1.0 5,442,627 + Pf6N2E2_4709 0.19 +0.5 5,442,628 - Pf6N2E2_4709 0.19 +0.0 5,442,628 - Pf6N2E2_4709 0.19 +0.1 5,442,628 - Pf6N2E2_4709 0.19 +0.7 5,442,628 - Pf6N2E2_4709 0.19 -1.1 5,442,628 - Pf6N2E2_4709 0.19 +1.1 5,442,628 - Pf6N2E2_4709 0.19 -0.8 5,442,628 - Pf6N2E2_4709 0.19 -0.7 5,442,727 - Pf6N2E2_4709 0.22 +0.2
Or see this region's nucleotide sequence