Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4622 and Pf6N2E2_4623 overlap by 4 nucleotides Pf6N2E2_4623 and Pf6N2E2_4624 overlap by 4 nucleotides Pf6N2E2_4624 and Pf6N2E2_4625 overlap by 4 nucleotides Pf6N2E2_4625 and Pf6N2E2_4626 are separated by 67 nucleotides
Pf6N2E2_4622 - Malonate decarboxylase beta subunit, at 5,351,960 to 5,352,811
_4622
Pf6N2E2_4623 - Malonate decarboxylase gamma subunit, at 5,352,808 to 5,353,611
_4623
Pf6N2E2_4624 - Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-), at 5,353,608 to 5,354,228
_4624
Pf6N2E2_4625 - Malonyl CoA acyl carrier protein transacylase (EC 2.3.1.39), at 5,354,225 to 5,355,145
_4625
Pf6N2E2_4626 - Malonate transporter, MadL subunit, at 5,355,213 to 5,355,629
_4626
Position (kb)
5353
5354
5355 Strain fitness (log2 ratio)
-1
0
1 at 5352.629 kb on + strand at 5352.629 kb on + strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.630 kb on - strand, within Pf6N2E2_4622 at 5352.791 kb on + strand at 5352.792 kb on - strand at 5352.817 kb on + strand at 5352.818 kb on - strand at 5353.108 kb on + strand, within Pf6N2E2_4623 at 5353.109 kb on - strand, within Pf6N2E2_4623 at 5353.109 kb on - strand, within Pf6N2E2_4623 at 5353.168 kb on + strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.169 kb on - strand, within Pf6N2E2_4623 at 5353.253 kb on - strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.390 kb on + strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.391 kb on - strand, within Pf6N2E2_4623 at 5353.558 kb on + strand at 5353.559 kb on - strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.613 kb on + strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.614 kb on - strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.617 kb on + strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.618 kb on - strand at 5353.789 kb on - strand, within Pf6N2E2_4624 at 5354.450 kb on + strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.451 kb on - strand, within Pf6N2E2_4625 at 5354.564 kb on + strand, within Pf6N2E2_4625 at 5354.873 kb on + strand, within Pf6N2E2_4625 at 5354.985 kb on - strand, within Pf6N2E2_4625 at 5355.143 kb on + strand at 5355.143 kb on + strand at 5355.206 kb on + strand at 5355.206 kb on + strand at 5355.207 kb on - strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.218 kb on + strand at 5355.219 kb on - strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.222 kb on + strand at 5355.223 kb on - strand at 5355.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 5,352,629 + -1.6 5,352,629 + Pf6N2E2_4622 0.79 -0.1 5,352,630 - Pf6N2E2_4622 0.79 +0.2 5,352,630 - Pf6N2E2_4622 0.79 +0.0 5,352,630 - Pf6N2E2_4622 0.79 +0.3 5,352,630 - Pf6N2E2_4622 0.79 +0.4 5,352,791 + -0.3 5,352,792 - -0.1 5,352,817 + +0.2 5,352,818 - -0.9 5,353,108 + Pf6N2E2_4623 0.37 -0.8 5,353,109 - Pf6N2E2_4623 0.37 +0.6 5,353,109 - Pf6N2E2_4623 0.37 -0.0 5,353,168 + Pf6N2E2_4623 0.45 -0.1 5,353,169 - Pf6N2E2_4623 0.45 +0.2 5,353,169 - Pf6N2E2_4623 0.45 -0.2 5,353,169 - Pf6N2E2_4623 0.45 -0.3 5,353,253 - Pf6N2E2_4623 0.55 +1.0 5,353,390 + Pf6N2E2_4623 0.72 +0.2 5,353,390 + Pf6N2E2_4623 0.72 -1.1 5,353,390 + Pf6N2E2_4623 0.72 +0.5 5,353,391 - Pf6N2E2_4623 0.73 +0.1 5,353,391 - Pf6N2E2_4623 0.73 +0.1 5,353,391 - Pf6N2E2_4623 0.73 +0.2 5,353,391 - Pf6N2E2_4623 0.73 -0.4 5,353,391 - Pf6N2E2_4623 0.73 +0.7 5,353,558 + -0.1 5,353,559 - -0.5 5,353,613 + -0.5 5,353,613 + -0.3 5,353,613 + +0.7 5,353,613 + +0.6 5,353,613 + -0.4 5,353,613 + -0.1 5,353,614 - +0.0 5,353,614 - -0.1 5,353,614 - +0.6 5,353,614 - -0.8 5,353,614 - +0.3 5,353,614 - -0.7 5,353,617 + -0.8 5,353,617 + -0.2 5,353,617 + -0.3 5,353,617 + -0.4 5,353,617 + -0.4 5,353,617 + -0.3 5,353,617 + -0.3 5,353,617 + +0.3 5,353,617 + +0.7 5,353,617 + +0.6 5,353,617 + -1.0 5,353,618 - +0.7 5,353,618 - +0.2 5,353,618 - -1.5 5,353,618 - +0.2 5,353,618 - -0.6 5,353,618 - +0.2 5,353,618 - +0.3 5,353,618 - +0.3 5,353,618 - +0.4 5,353,618 - +0.8 5,353,789 - Pf6N2E2_4624 0.29 -0.8 5,354,450 + Pf6N2E2_4625 0.24 -0.4 5,354,451 - Pf6N2E2_4625 0.25 -0.1 5,354,451 - Pf6N2E2_4625 0.25 +0.6 5,354,451 - Pf6N2E2_4625 0.25 -0.0 5,354,564 + Pf6N2E2_4625 0.37 +1.7 5,354,873 + Pf6N2E2_4625 0.70 +0.0 5,354,985 - Pf6N2E2_4625 0.83 +0.1 5,355,143 + -0.3 5,355,143 + -0.4 5,355,206 + -0.7 5,355,206 + +0.2 5,355,207 - +0.7 5,355,218 + +0.4 5,355,218 + +0.0 5,355,218 + +0.2 5,355,218 + +0.5 5,355,218 + -0.1 5,355,219 - -0.3 5,355,222 + +0.7 5,355,222 + +0.7 5,355,222 + +0.1 5,355,222 + +0.1 5,355,223 - +0.5 5,355,223 - +0.0
Or see this region's nucleotide sequence