Experiment: N1B4wt with N2E2m denitrifying replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_369 and Pf6N2E2_370 are separated by 131 nucleotides Pf6N2E2_370 and Pf6N2E2_371 are separated by 41 nucleotides Pf6N2E2_371 and Pf6N2E2_372 are separated by 225 nucleotides
Pf6N2E2_369 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 436,538 to 437,617
_369
Pf6N2E2_370 - Predicted enzyme of the cupin superfamily, at 437,749 to 438,456
_370
Pf6N2E2_371 - Histone acetyltransferase HPA2 and related acetyltransferases, at 438,498 to 439,355
_371
Pf6N2E2_372 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 439,581 to 440,507
_372
Position (kb) 438
439
440 Strain fitness (log2 ratio) -2
-1
0
1
2 at 437.740 kb on + strand at 437.751 kb on - strand at 437.763 kb on - strand at 437.763 kb on - strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.777 kb on + strand at 437.778 kb on - strand at 437.821 kb on + strand, within Pf6N2E2_370 at 437.869 kb on + strand, within Pf6N2E2_370 at 437.869 kb on + strand, within Pf6N2E2_370 at 437.937 kb on - strand, within Pf6N2E2_370 at 437.937 kb on - strand, within Pf6N2E2_370 at 437.956 kb on - strand, within Pf6N2E2_370 at 437.956 kb on - strand, within Pf6N2E2_370 at 438.238 kb on + strand, within Pf6N2E2_370 at 438.345 kb on - strand, within Pf6N2E2_370 at 438.685 kb on - strand, within Pf6N2E2_371 at 438.984 kb on - strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.015 kb on + strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.016 kb on - strand, within Pf6N2E2_371 at 439.117 kb on + strand, within Pf6N2E2_371 at 439.118 kb on - strand, within Pf6N2E2_371 at 439.325 kb on - strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.330 kb on + strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.331 kb on - strand at 439.373 kb on + strand at 439.415 kb on + strand at 439.416 kb on - strand at 439.416 kb on - strand at 439.526 kb on + strand at 439.526 kb on + strand at 439.526 kb on + strand at 439.527 kb on - strand at 439.527 kb on - strand at 439.585 kb on + strand at 439.585 kb on + strand at 439.586 kb on - strand at 439.589 kb on + strand at 439.590 kb on - strand at 439.710 kb on + strand, within Pf6N2E2_372 at 439.710 kb on + strand, within Pf6N2E2_372 at 439.710 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.938 kb on + strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 439.939 kb on - strand, within Pf6N2E2_372 at 440.067 kb on + strand, within Pf6N2E2_372 at 440.215 kb on + strand, within Pf6N2E2_372 at 440.274 kb on - strand, within Pf6N2E2_372
Per-strain Table
Position Strand Gene LocusTag Fraction N1B4wt with N2E2m denitrifying replicate C remove 437,740 + -0.1 437,751 - -0.5 437,763 - -0.1 437,763 - -1.9 437,777 + +1.2 437,777 + -0.9 437,777 + +1.0 437,777 + +0.1 437,777 + +0.0 437,778 - -0.4 437,821 + Pf6N2E2_370 0.10 -0.3 437,869 + Pf6N2E2_370 0.17 -0.4 437,869 + Pf6N2E2_370 0.17 -0.4 437,937 - Pf6N2E2_370 0.27 -0.9 437,937 - Pf6N2E2_370 0.27 -1.1 437,956 - Pf6N2E2_370 0.29 -0.9 437,956 - Pf6N2E2_370 0.29 -0.9 438,238 + Pf6N2E2_370 0.69 +0.1 438,345 - Pf6N2E2_370 0.84 -0.1 438,685 - Pf6N2E2_371 0.22 +0.0 438,984 - Pf6N2E2_371 0.57 +0.6 439,015 + Pf6N2E2_371 0.60 -0.0 439,015 + Pf6N2E2_371 0.60 -0.1 439,016 - Pf6N2E2_371 0.60 +0.6 439,016 - Pf6N2E2_371 0.60 +1.7 439,016 - Pf6N2E2_371 0.60 +1.8 439,117 + Pf6N2E2_371 0.72 +1.0 439,118 - Pf6N2E2_371 0.72 -0.9 439,325 - +0.2 439,330 + +0.9 439,330 + -1.3 439,330 + +0.4 439,330 + -0.3 439,330 + +0.4 439,331 - +0.9 439,331 - -0.3 439,331 - -0.1 439,373 + +0.0 439,415 + +0.8 439,416 - +0.0 439,416 - +0.3 439,526 + -0.9 439,526 + -0.4 439,526 + -0.0 439,527 - -0.6 439,527 - +0.1 439,585 + -1.0 439,585 + +1.0 439,586 - -0.5 439,589 + -0.7 439,590 - +0.2 439,710 + Pf6N2E2_372 0.14 +0.2 439,710 + Pf6N2E2_372 0.14 -0.7 439,710 + Pf6N2E2_372 0.14 +0.2 439,938 + Pf6N2E2_372 0.39 -0.7 439,938 + Pf6N2E2_372 0.39 +0.2 439,938 + Pf6N2E2_372 0.39 -0.2 439,939 - Pf6N2E2_372 0.39 -0.9 439,939 - Pf6N2E2_372 0.39 -0.3 439,939 - Pf6N2E2_372 0.39 +0.7 439,939 - Pf6N2E2_372 0.39 -0.6 440,067 + Pf6N2E2_372 0.52 +0.6 440,215 + Pf6N2E2_372 0.68 -0.1 440,274 - Pf6N2E2_372 0.75 -1.1
Or see this region's nucleotide sequence