Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5964 and Pf1N1B4_5965 are separated by 11 nucleotides Pf1N1B4_5965 and Pf1N1B4_5966 overlap by 8 nucleotides Pf1N1B4_5966 and Pf1N1B4_5967 overlap by 4 nucleotides
Pf1N1B4_5964 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 412,354 to 413,286
_5964
Pf1N1B4_5965 - D-amino-acid oxidase (EC 1.4.3.3), at 413,298 to 414,398
_5965
Pf1N1B4_5966 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 414,391 to 414,627
_5966
Pf1N1B4_5967 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 414,624 to 415,880
_5967
Position (kb) 413
414
415 Strain fitness (log2 ratio) -2
-1
0
1
2 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.909 kb on - strand at 412.909 kb on - strand, within Pf1N1B4_5964 at 412.912 kb on + strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.944 kb on + strand, within Pf1N1B4_5964 at 412.944 kb on + strand, within Pf1N1B4_5964 at 412.946 kb on + strand at 412.946 kb on + strand, within Pf1N1B4_5964 at 412.946 kb on + strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 413.170 kb on + strand, within Pf1N1B4_5964 at 413.171 kb on - strand, within Pf1N1B4_5964 at 413.171 kb on - strand, within Pf1N1B4_5964 at 413.221 kb on - strand at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.894 kb on - strand, within Pf1N1B4_5965 at 413.958 kb on - strand, within Pf1N1B4_5965 at 413.976 kb on + strand, within Pf1N1B4_5965 at 413.977 kb on - strand at 414.023 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.139 kb on - strand, within Pf1N1B4_5965 at 414.391 kb on - strand at 414.918 kb on + strand, within Pf1N1B4_5967 at 414.919 kb on - strand, within Pf1N1B4_5967 at 414.955 kb on + strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 412,907 - Pf1N1B4_5964 0.59 -1.4 412,907 - Pf1N1B4_5964 0.59 -0.9 412,907 - Pf1N1B4_5964 0.59 -0.8 412,908 + Pf1N1B4_5964 0.59 -0.8 412,908 + Pf1N1B4_5964 0.59 +0.6 412,908 + Pf1N1B4_5964 0.59 +1.0 412,908 + Pf1N1B4_5964 0.59 -1.4 412,908 + Pf1N1B4_5964 0.59 -1.9 412,908 + Pf1N1B4_5964 0.59 -1.9 412,908 + Pf1N1B4_5964 0.59 +0.3 412,909 - -0.4 412,909 - Pf1N1B4_5964 0.59 +0.9 412,912 + Pf1N1B4_5964 0.60 -0.1 412,913 - Pf1N1B4_5964 0.60 -0.9 412,913 - Pf1N1B4_5964 0.60 +0.0 412,913 - Pf1N1B4_5964 0.60 -0.4 412,913 - Pf1N1B4_5964 0.60 +0.6 412,944 + Pf1N1B4_5964 0.63 +0.6 412,944 + Pf1N1B4_5964 0.63 +0.6 412,946 + +1.2 412,946 + Pf1N1B4_5964 0.63 +2.0 412,946 + Pf1N1B4_5964 0.63 -0.4 412,947 - Pf1N1B4_5964 0.64 -1.9 412,947 - Pf1N1B4_5964 0.64 -0.4 412,947 - Pf1N1B4_5964 0.64 +2.1 413,170 + Pf1N1B4_5964 0.87 +0.1 413,171 - Pf1N1B4_5964 0.88 -0.4 413,171 - Pf1N1B4_5964 0.88 -1.4 413,221 - +0.6 413,595 + Pf1N1B4_5965 0.27 -0.4 413,595 + Pf1N1B4_5965 0.27 -0.8 413,595 + Pf1N1B4_5965 0.27 -2.4 413,595 + Pf1N1B4_5965 0.27 -1.8 413,894 - Pf1N1B4_5965 0.54 +0.3 413,958 - Pf1N1B4_5965 0.60 -1.4 413,976 + Pf1N1B4_5965 0.62 -0.9 413,977 - -0.4 414,023 + Pf1N1B4_5965 0.66 -0.4 414,138 + Pf1N1B4_5965 0.76 -1.2 414,138 + Pf1N1B4_5965 0.76 -0.0 414,139 - Pf1N1B4_5965 0.76 +0.4 414,391 - +0.3 414,918 + Pf1N1B4_5967 0.23 +0.2 414,919 - Pf1N1B4_5967 0.23 -0.6 414,955 + Pf1N1B4_5967 0.26 +1.6 414,956 - Pf1N1B4_5967 0.26 +0.5 414,956 - Pf1N1B4_5967 0.26 +0.6 414,956 - Pf1N1B4_5967 0.26 -1.4
Or see this region's nucleotide sequence