Experiment: Cephalothin 2.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5859 and Pf1N1B4_5860 are separated by 213 nucleotides Pf1N1B4_5860 and Pf1N1B4_5861 are separated by 555 nucleotides
Pf1N1B4_5859 - Glutamate carboxypeptidase (EC 3.4.17.11), at 292,416 to 293,657
_5859
Pf1N1B4_5860 - Two-component response regulator, at 293,871 to 294,806
_5860
Pf1N1B4_5861 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 295,362 to 296,288
_5861
Position (kb)
293
294
295 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 293.067 kb on + strand, within Pf1N1B4_5859 at 293.068 kb on - strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.200 kb on + strand, within Pf1N1B4_5859 at 293.201 kb on - strand at 293.201 kb on - strand, within Pf1N1B4_5859 at 293.534 kb on - strand at 293.695 kb on + strand at 293.695 kb on + strand at 293.793 kb on + strand at 293.793 kb on + strand at 294.061 kb on + strand, within Pf1N1B4_5860 at 294.198 kb on + strand, within Pf1N1B4_5860 at 294.199 kb on - strand, within Pf1N1B4_5860 at 294.251 kb on + strand, within Pf1N1B4_5860 at 294.252 kb on - strand, within Pf1N1B4_5860 at 294.499 kb on + strand, within Pf1N1B4_5860 at 294.562 kb on - strand at 295.007 kb on + strand at 295.051 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.093 kb on + strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.094 kb on - strand at 295.139 kb on + strand at 295.140 kb on - strand at 295.140 kb on - strand at 295.440 kb on + strand at 295.441 kb on - strand at 295.575 kb on + strand, within Pf1N1B4_5861 at 295.641 kb on - strand, within Pf1N1B4_5861 at 295.756 kb on + strand, within Pf1N1B4_5861 at 295.757 kb on - strand, within Pf1N1B4_5861
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2.5 mg/ml remove 293,067 + Pf1N1B4_5859 0.52 +0.8 293,068 - Pf1N1B4_5859 0.52 +2.0 293,200 + Pf1N1B4_5859 0.63 +0.2 293,200 + Pf1N1B4_5859 0.63 +1.2 293,200 + Pf1N1B4_5859 0.63 +0.1 293,200 + Pf1N1B4_5859 0.63 -1.9 293,200 + Pf1N1B4_5859 0.63 +0.6 293,200 + Pf1N1B4_5859 0.63 -0.6 293,201 - -0.4 293,201 - Pf1N1B4_5859 0.63 +1.2 293,534 - +1.0 293,695 + -1.7 293,695 + -0.4 293,793 + +0.6 293,793 + +0.6 294,061 + Pf1N1B4_5860 0.20 -0.9 294,198 + Pf1N1B4_5860 0.35 -0.4 294,199 - Pf1N1B4_5860 0.35 -0.8 294,251 + Pf1N1B4_5860 0.41 -0.7 294,252 - Pf1N1B4_5860 0.41 -1.2 294,499 + Pf1N1B4_5860 0.67 +0.6 294,562 - -1.4 295,007 + -1.9 295,051 + -0.2 295,093 + -0.6 295,093 + +1.3 295,093 + +1.3 295,093 + -1.4 295,093 + +0.6 295,093 + -0.8 295,093 + +1.2 295,093 + +2.0 295,093 + -0.7 295,093 + +1.1 295,094 - -0.4 295,094 - +0.2 295,094 - -0.7 295,094 - -1.7 295,094 - -0.1 295,094 - +0.1 295,094 - +1.2 295,094 - +0.6 295,094 - +0.9 295,139 + -0.7 295,140 - -0.5 295,140 - +0.1 295,440 + +0.2 295,441 - +0.5 295,575 + Pf1N1B4_5861 0.23 -0.4 295,641 - Pf1N1B4_5861 0.30 +1.1 295,756 + Pf1N1B4_5861 0.43 -1.9 295,757 - Pf1N1B4_5861 0.43 -1.6
Or see this region's nucleotide sequence